公駝?wù)硐俜置谖飳?duì)小鼠免疫功能的影響及抗腫瘤作用研究
發(fā)布時(shí)間：2018-06-06 19:46
本文選題：公駝?wù)硐俜置谖?/strong> + 免疫抑制小鼠��； 參考：《內(nèi)蒙古農(nóng)業(yè)大學(xué)》2012年碩士論文
【摘要】：目的：研究公駝?wù)硐俜置谖?poll gland secretion, PGS)對(duì)免疫抑制小鼠免疫功能的影響及對(duì)荷瘤小鼠的抗腫瘤作用。方法：采用腹腔注射環(huán)磷酰胺法制造免疫抑制小鼠模型,運(yùn)用S180細(xì)胞懸液腋窩皮下接種法構(gòu)建小鼠荷瘤模型。對(duì)于免疫抑制模型實(shí)驗(yàn)小鼠,隨機(jī)分為正常組,環(huán)磷酰胺模型對(duì)照組,PGS高(250mg/kg).中(25mg/kg).低(2.5mg/kg)劑量組,灌胃給予藥物,每日1次,用藥14d后處死小鼠。稱量各組小鼠體重、計(jì)算免疫器官指數(shù),并采用ELISA法檢測(cè)血清中IL-6和TNF-α以及IgG和IgM的含量：采用碳粒廓清法測(cè)定各組小鼠單核巨噬細(xì)胞系統(tǒng)的吞噬功能。對(duì)于S180荷瘤小鼠模型,隨機(jī)分成5組：腫瘤模型陰性對(duì)照組、PGS高、中、低劑量處理組(250mg/kg.25mg／kg口2.5mg/kg)及環(huán)磷酰胺陽(yáng)性對(duì)照處理組,每只小鼠右前肢腋窩皮下分別接種0.2mL從荷瘤小鼠腹腔中取得的瘤細(xì)胞液(用生理鹽水調(diào)整到含瘤細(xì)胞數(shù)1×107個(gè)／mL)。觀察小鼠的狀態(tài)及腫瘤體積大小,用藥28d,于第29d處死小鼠,對(duì)其進(jìn)行解剖,稱量小鼠實(shí)體瘤重量,并計(jì)算抑瘤率,并用ELISA法檢測(cè)IL-2、IL-6、TNF-α、IgG和IgM等相關(guān)免疫指標(biāo),對(duì)解剖所得實(shí)體瘤組織做HE染色石蠟切片,在顯微鏡下進(jìn)行組織切片觀察,采用MTT法檢測(cè)PGS對(duì)S180細(xì)胞抑制率。結(jié)果：與環(huán)磷酰胺模型對(duì)照組相比,中、高劑量PGS能顯著提高免疫抑制小鼠胸腺指數(shù)和脾臟指數(shù)(P0.05),顯著提高血清中IL-6含量(P0.05)和IgG含量；高劑量PGS能顯著提高小鼠血清TNF-α的含量(P0.05),低、中劑量PGS組小鼠血清中TNF-α含量亦有上升趨勢(shì)：PGS高劑量組小鼠單核巨噬細(xì)胞吞噬能力顯著高于環(huán)磷酰胺模型對(duì)照組小鼠；高劑量PGS對(duì)荷瘤小鼠的腫瘤生長(zhǎng)具有一定的抑制作用,石蠟切片的結(jié)果也顯示高劑量PGS具有一定的抑制腫瘤生長(zhǎng)作用；同時(shí),中高劑量PGS對(duì)提高荷瘤小鼠血清TNF-α和IL-2和IL-6等細(xì)胞因子也具有提升作用；對(duì)于荷瘤小鼠,IgG含量的變化不是很明顯,而IgM含量則出現(xiàn)了逆向增長(zhǎng)的情況,顯示PGS對(duì)體液免疫中免疫球蛋白的作用比較復(fù)雜。結(jié)論：一定劑量PGS可明顯增加免疫抑制小鼠免疫器官的相對(duì)重量,提高臟器指數(shù),并可顯著提高免疫抑制小鼠血清TNF-α和IL-6等細(xì)胞因子以及IgM和IgG含量。PGS對(duì)荷瘤小鼠腫瘤生長(zhǎng)具有一定的抑制作用,抑制作用呈現(xiàn)較明顯的量效關(guān)系：高劑量PGS對(duì)荷瘤小鼠腫瘤的生長(zhǎng)具有較為顯著的抑制作用,同時(shí)可顯著提高荷瘤小鼠血清TNF-α、IL-2和IL-6等細(xì)胞因子水平。一定劑量的PGS對(duì)免疫抑制小鼠和荷瘤小鼠的特異性免疫和非特異性免疫均有增強(qiáng)作用。
[Abstract]:Aim: to study the effect of male camel occipital gland secretion poll gland secretion (PGSs) on immune function of immunosuppressive mice and its antitumor effect on tumor-bearing mice. Methods: the immunosuppressive mice model was established by intraperitoneal injection of cyclophosphamide and subcutaneous inoculation of S180 cell suspension. The immunosuppressive mice were randomly divided into normal control group and cyclophosphamide model control group with PGS of 250 mg / kg 路kg ~ (-1). In the middle of 25 mg / kg. The mice in the low dose group (2.5 mg / kg) were given intragastric administration once a day, and the mice were killed after 14 days of administration. The body weight, immune organ index, serum IL-6, TNF- 偽, IgG and IgM were measured by Elisa. The phagocytic function of mononuclear macrophage system in each group was determined by carbon particle clearance method. S180 tumor-bearing mice were randomly divided into 5 groups: the tumor model negative control group was treated with 250 mg / kg / kg 2.5 mg / kg PGS and the cyclophosphamide positive control group. Each mouse was subcutaneously inoculated with 0.2 mL tumor cell fluid (adjusted with normal saline to 1 脳 107 mLX) from the abdominal cavity of the tumor-bearing mice. The state and tumor volume of the mice were observed. The mice were killed on the 29th day after 28 days of administration. The mice were dissected and weighed, and the tumor inhibition rate was calculated. The immunological indexes such as IL-2mil, IL-6, TNF- 偽 IgG and IgM were detected by Elisa. The paraffin sections of solid tumor tissue were stained with HE and observed under microscope. The inhibitory rate of PGS on S180 cells was detected by MTT method. Results: compared with cyclophosphamide model control group, high dose PGS could significantly increase thymus index and spleen index of immunosuppressive mice (P 0.05), increase serum IL-6 content (P 0.05) and IgG content. The content of TNF- 偽 in the serum of mice was significantly increased by high dose PGS, and the content of TNF- 偽 in the serum of medium dose PGS group was significantly higher than that of cyclophosphamide model group. The level of TNF- 偽 in the serum of mice in the middle dose PGS group was also increased. The phagocytosis ability of mononuclear macrophages in the high dose group was significantly higher than that in the control group. High dose PGS could inhibit tumor growth in tumor-bearing mice, and paraffin section showed that high dose PGS could inhibit tumor growth. The medium and high dose of PGS also increased the cytokines such as TNF- 偽, IL-2 and IL-6 in tumor bearing mice, but the change of IgG content in tumor bearing mice was not obvious, but the IgM content increased inversely. The results showed that the effect of PGS on immunoglobulin in humoral immunity was complicated. Conclusion: a certain dose of PGS can significantly increase the relative weight of immune organs and increase the visceral index of immunosuppressive mice. Serum TNF- 偽 and IL-6 and IgM and IgG contents in immunosuppressive mice were significantly increased. PGS could inhibit tumor growth in tumor-bearing mice to some extent. The inhibitory effect showed a dose-effect relationship: high dose of PGS could significantly inhibit tumor growth in tumor-bearing mice and increase the levels of cytokines such as TNF- 偽, IL-2 and IL-6 in serum of tumor-bearing mice. A certain dose of PGS enhanced the specific and nonspecific immunity of immunosuppressive mice and tumor-bearing mice.
【學(xué)位授予單位】：內(nèi)蒙古農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332;R285.5
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