本文選題：旋毛蟲 + 成蟲��； 參考：《中國(guó)寄生蟲學(xué)與寄生蟲病雜志》2017年01期

【摘要】：目的通過(guò)"鳥槍法"(shotgun技術(shù))分析旋毛蟲(Trichinella spiralis)成蟲排泄分泌蛋白(adult worm excretory/secretory protein,AWESP)的組分,尋找其調(diào)節(jié)人炎癥性腸炎的活性成分。方法制備旋毛蟲AWESP,經(jīng)十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)分離酶解后,采用shotgun液相色譜-串聯(lián)質(zhì)譜系統(tǒng)鑒定,將分析所得的數(shù)據(jù)通過(guò)Masco軟件進(jìn)行數(shù)據(jù)庫(kù)檢索,完成蛋白的鑒定,并對(duì)鑒定出的蛋白從細(xì)胞組分、分子功能和生物學(xué)途徑3個(gè)方面進(jìn)行Gene Ontology(GO)分類。結(jié)果 SDS-PAGE分離的旋毛蟲AWESP相對(duì)分子質(zhì)量(Mr)約15 000~116 000,質(zhì)譜分析共獲得280個(gè)蛋白,通過(guò)Masco軟件檢索已鑒定的蛋白為96種,假定的蛋白為98種,未鑒定的蛋白為86種。已初步發(fā)現(xiàn)至少有4種蛋白可能具有潛在的調(diào)節(jié)人炎癥性腸炎的作用,包括半胱氨酸蛋白酶抑制劑、絲氨酸蛋白酶、53 000排泄/分泌抗原和谷胱氨肽轉(zhuǎn)移酶。GO工具分析顯示,已鑒定的蛋白具有104種分子功能,參與了363種生物過(guò)程。結(jié)論經(jīng)Masco軟件檢索,旋毛蟲AWESP成分復(fù)雜多樣,已鑒定的蛋白有96種,從已明確的蛋白中初步篩選出4種與調(diào)節(jié)人炎癥性腸炎潛在相關(guān)的蛋白。
[Abstract]:Objective to analyze the components of adult worm excretory/secretory protein AWESPs of Trichinella spiralisi (Trichinella spiralisi) by "shotgun technique", and to find out the active components of adult worm excretory/secretory protein AWESPs regulating human inflammatory enteritis. Methods the Trichinella spiralis AWESPS was prepared and hydrolyzed by SDS-PAGE. the data were identified by shotgun liquid chromatography-tandem mass spectrometry. The data were searched by Masco software. The identified proteins were classified by Gene Ontology (GOG) from three aspects: cellular components, molecular functions and biological pathways. Results the relative molecular weight of AWESP isolated from Trichinella spiralis by SDS-PAGE was about 15000 ~ 116,000. A total of 280 proteins were obtained by mass spectrometry. 96 proteins were identified by Masco software, 98 were assumed proteins and 86 were unidentified proteins. At least four proteins have been preliminarily found to have a potential role in regulating human inflammatory enteritis, including cysteine protease inhibitors, serine protease 53,000 excretory / secretory antigens and glutathione transferase .go tool analysis. The identified proteins have 104 molecular functions and participate in 363 biological processes. Conclusion by Masco software, Trichinella spiralis AWESP has a complex and diverse composition, and 96 proteins have been identified. Four proteins related to the regulation of human inflammatory enteritis were preliminarily screened from the identified proteins.
【作者單位】： 蚌埠醫(yī)學(xué)院病原生物學(xué)教研室安徽省感染與免疫重點(diǎn)實(shí)驗(yàn)室;江南大學(xué)無(wú)錫醫(yī)學(xué)院病原感染與免疫組;
【基金】：國(guó)家自然科學(xué)基金(No.81441120) 安徽省自然科學(xué)基金(No.1508085QH158) 安徽高�？蒲袆�(chuàng)新平臺(tái)團(tuán)隊(duì)項(xiàng)目(2016-40) 安徽省高校優(yōu)秀青年人才支持計(jì)劃重點(diǎn)項(xiàng)目(No.gxyq ZD2016159) 安徽省高等學(xué)校自然科學(xué)研究重點(diǎn)項(xiàng)目(No.KJ2016A708,KJ2015A146)~~
【分類號(hào)】：R383.15
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本文編號(hào)：1981381