發(fā)布時(shí)間：2018-06-02 12:09

  本文選題：脂肪間充質(zhì)干細(xì)胞 + 成骨轉(zhuǎn)化�。� 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：觀察兔不同部位來(lái)源的脂肪間充質(zhì)干細(xì)胞（ADSCs）分化成骨的能力。 方法：分別從一只新西蘭兔的腹股溝、腹膜后、頸背部取出脂肪組織并提取脂肪間充質(zhì)干細(xì)胞，體外培養(yǎng)傳至第5代，加入誘導(dǎo)劑分別進(jìn)行成骨誘導(dǎo)，不加入誘導(dǎo)劑的為對(duì)照組。 結(jié)果：經(jīng)鈣鈷染色及茜素紅染色后，誘導(dǎo)組大部分細(xì)胞呈陽(yáng)性反應(yīng)，以腹股溝部脂肪間充質(zhì)干細(xì)胞的結(jié)節(jié)體積最大，對(duì)照組未見(jiàn)明顯陽(yáng)性細(xì)胞。細(xì)胞內(nèi)堿性磷酸酶（ALP）活性檢測(cè)表明誘導(dǎo)組比對(duì)照組ALP活性明顯升高（P＜0.05）；腹股溝部脂肪間充質(zhì)干細(xì)胞ALP活性較頸背部及腹膜后高，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）；頸背部與腹膜后相比較無(wú)明顯差異（P＞0.05）。 結(jié)論：1、兔ADSCs經(jīng)過(guò)體外誘導(dǎo)后，，具有成骨能力。2、腹股溝部脂肪可作為骨組織工程種子細(xì)胞來(lái)源的較佳取材部位。
[Abstract]:Aim: to observe the ability of adipose mesenchymal stem cells (ADSCs) derived from different parts of rabbit to differentiate into bone. Methods: adipose tissue and adipose mesenchymal stem cells were extracted from the groin and peritoneum of a New Zealand rabbit respectively. The adipose mesenchymal stem cells were cultured to the fifth passage in vitro. The control group did not add the inducer. Results: after calcium cobalt staining and alizarin red staining, most of the cells in the induction group showed positive reaction, and the tubule size of the inguinal adipose mesenchymal stem cells was the largest, but no obvious positive cells were found in the control group. The activity of alkaline phosphatase (ALP) in the induction group was significantly higher than that in the control group (P < 0.05), and the ALP activity in the inguinal adipose mesenchymal stem cells was higher than that in the back of neck and retroperitoneum. The difference was statistically significant (P < 0.05), but there was no significant difference between the back of neck and retroperitoneum (P > 0.05). Conclusion ADSCs has the ability of osteogenesis in vitro. The inguinal fat can be used as the best source of seed cells for bone tissue engineering.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R329

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 周紅星,楊柳,陳光興,顧祖超,李起鴻;骨髓基質(zhì)細(xì)胞載體支架的細(xì)胞相容性特點(diǎn)[J];中國(guó)臨床康復(fù);2005年14期

2 魏寬海,裴國(guó)獻(xiàn),鄭磊,王前,金丹,胡罷生;地塞米松對(duì)骨髓基質(zhì)細(xì)胞生物學(xué)特性的影響[J];中國(guó)修復(fù)重建外科雜志;2001年04期

3 黎洪棉;高建華;魯峰;李華;;地塞米松在家兔脂肪基質(zhì)干細(xì)胞定向誘導(dǎo)為成骨細(xì)胞的作用[J];中國(guó)組織工程研究與臨床康復(fù);2007年20期

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