本文選題：自擬更年湯 + 腎陽虛�。� 參考：《現(xiàn)代中西醫(yī)結(jié)合雜志》2016年02期

【摘要】：目的探討自擬更年湯對腎陽虛型圍絕經(jīng)期模型大鼠的雌激素樣作用機制。方法選擇陰道細胞學(xué)表現(xiàn)為有規(guī)律的4~5 d動情周期的4月齡雌性SD大鼠20只為青年組,陰道細胞學(xué)表現(xiàn)無動情周期改變的18月齡雌性SD大鼠20只為老年組;選用自然老化的圍絕經(jīng)期雌性SD大鼠,肌注氫化可的松構(gòu)建腎陽虛型圍絕經(jīng)期大鼠模型,將造模成功大鼠隨機分為更年湯組、教材方組、利維愛組和生理鹽水組。青年組、老年組、生理鹽水組均給予生理鹽水灌胃,各給藥組給予相應(yīng)藥物灌胃,灌胃15 d后處死大鼠,分離摘取子宮稱子宮濕質(zhì)量,計算子宮指數(shù),采用免疫組化及原位雜交檢測方法觀察大鼠子宮組織中血管內(nèi)皮生長因子(VEGF)及其信使VEGF mRNA的陽性表達情況。結(jié)果各給藥組子宮濕質(zhì)量、子宮指數(shù)均明顯高于生理鹽水組和老年組(P均0.05);更年湯組子宮濕質(zhì)量、子宮指數(shù)與青年組比較差異均無統(tǒng)計學(xué)意義(P均0.05),與利維愛組、教材方組比較差異均有統(tǒng)計學(xué)意義(P均0.05);利維愛組子宮濕質(zhì)量、子宮指數(shù)與教材方組比較差異均無統(tǒng)計學(xué)意義(P均0.05)。老年組和生理鹽水組VEGF及VEGF mRNA陽性表達明顯低于青年組(P均0.05)。各給藥組VEGF及VEGF mRNA的陽性表達明顯高于生理鹽水組、老年組(P均0.05),更年湯組、利維愛組VEGF及VEGF mRNA陽性表達明顯高于教材方組(P均0.05),利維愛組VEGF及VEGF mRNA陽性表達與更年湯組比較差異均無統(tǒng)計學(xué)意義(P均0.05)。結(jié)論自擬更年湯可促進子宮增生質(zhì)量,具有雌激素樣作用;其促進子宮增加質(zhì)量的機制可能與上調(diào)子宮組織中VEGF及VEGFmRNA的陽性表達有關(guān)。
[Abstract]:Objective to investigate the estrogen-like mechanism of self-made Gengnian decoction on the peri-menopausal rats with deficiency of kidney yang. Methods Twenty 4-month-old female SD rats with regular 4-month estrus cycle and 20 18-month-old female Sprague-Dawley rats with no change in vaginal cytology were selected as the aged group. Female SD rats with natural aging menopausal period were injected hydrocortisone intramuscularly to construct the model of kidney yang deficiency type perimenopausal rats. The successful rats were randomly divided into three groups: Gannian decoction group, textbook group, Levy group and normal saline group. The young group, the old group and the normal saline group were all given normal saline intragastric perfusion. The rats were killed 15 days after gavage, and the uterine index was calculated. The expression of vascular endothelial growth factor (VEGF) and its messenger VEGF mRNA in rat uterus were observed by immunohistochemistry and in situ hybridization. Results the uterine wet weight and uterine index in each administration group were significantly higher than those in normal saline group and senile group (P 0.05), and there was no significant difference in uterine wet mass and uterine index between Gannian decoction group and young group (P 0.05), and there was no significant difference between GND group and Levi group. There were significant differences between the teaching materials group and the textbook group (P < 0.05), while there was no significant difference in the uterine wet quality, uterine index and the textbook side group (P < 0.05) between the Levi group and the textbook group. The positive expression of VEGF and VEGF mRNA in the aged group and saline group was significantly lower than that in the young group (P 0.05). The positive expression of VEGF and VEGF mRNA in each administration group was significantly higher than that in normal saline group. The positive expression of VEGF and VEGF mRNA in Levi group was significantly higher than that in textbook group (P 0.05). There was no significant difference in VEGF and VEGF mRNA positive expression between Levi group and Gannian decoction group (P 0.05). Conclusion Self-made Gannian decoction can promote uterine hyperplasia and has estrogen-like effect, and its mechanism may be related to upregulation of VEGF and VEGFmRNA expression in uterine tissue.
【作者單位】： 貴陽中醫(yī)學(xué)院第二附屬醫(yī)院;貴陽中醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(81160491)
【分類號】：R285.5;R-332

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