本文選題：分枝桿菌 + 結(jié)核�。� 參考：《中華疾病控制雜志》2017年01期

【摘要】：目的描述武漢市耐多藥結(jié)核分枝桿菌基因型分布及成簇特征。方法選取15個(gè)結(jié)核分枝桿菌重復(fù)序列(mycobacterial interspersed repetitive unit,MIRU),對(duì)98株耐多藥結(jié)核分枝桿菌進(jìn)行多位點(diǎn)數(shù)目可變串聯(lián)重復(fù)序列分析(mycobacterial interspersed repetitive unit-variable number tandem repeat typing,MIRU-VNTR)分型,計(jì)算差異遺傳值、Hunter-Gaston指數(shù)(Hunter-Gaston discriminatory index,HGDI)、成簇率以及近期感染率最小估計(jì)值。結(jié)果MIRU位點(diǎn)組合的多態(tài)性差異遺傳值為0.960 4、HGDI為0.970 8,多態(tài)性和分辨指數(shù)最高的位點(diǎn)為Mtub 21及MIRU 26。共產(chǎn)生7個(gè)基因簇和65個(gè)獨(dú)立基因型,成簇率為33.67%,近期感染率最小估計(jì)為26.53%。結(jié)論 15位點(diǎn)MIRU-VNTR分型法分辨指數(shù)較高,適合于武漢地區(qū)耐多藥結(jié)核分枝桿菌的基因分型。盡管武漢市耐多藥肺結(jié)核病的流行多半歸因于內(nèi)源性復(fù)燃,但仍有較高比例的近期傳播。
[Abstract]:Objective to describe the genotype distribution and cluster characteristics of multi-drug resistant Mycobacterium tuberculosis in Wuhan. Methods A total of 15 Mycobacterium interspersed repetitive strains were selected and 98 strains of multidrug resistant Mycobacterium tuberculosis strains were analyzed by multilocus variable tandem repeat sequence analysis (MIRU-VNTRR) for mycobacterial interspersed repetitive unit-variable number tandem repeat typing. The Hunter-Gaston exponent Hunter-Gaston discriminatory index (HGDIN), the clustering rate and the minimum estimate of the infection rate in the near future were calculated. Results the polymorphic genetic value of MIRU locus combination was 0.960 4 HGDI = 0.970 8. The highest polymorphism and resolution index were Mtub 21 and MIRU 26. A total of 7 gene clusters and 65 independent genotypes were produced, and the clustering rate was 33.67. The lowest recent infection rate was estimated to be 26.53. Conclusion the 15 locus MIRU-VNTR method is suitable for multidrug resistant Mycobacterium tuberculosis genotyping in Wuhan. Although the prevalence of MDR-TB in Wuhan is mostly attributed to endogenous recurrence, there is still a high proportion of recent transmission.
【作者單位】： 武漢市結(jié)核病防治所結(jié)核病防控科;武漢市結(jié)核病防治所xZ口結(jié)核病防控科;武漢市結(jié)核病防治所檢驗(yàn)科;
【基金】：湖北省自然科學(xué)基金(2015CFB310) 湖北省衛(wèi)計(jì)委項(xiàng)目(WJ2015MB163) 武漢市衛(wèi)計(jì)委項(xiàng)目(WG14B10);武漢市衛(wèi)計(jì)委項(xiàng)目(WG15A04) 武漢市中青年醫(yī)學(xué)骨干人才項(xiàng)目([2014]77)
【分類號(hào)】：R378.911

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