本文選題：樹突狀細胞 + 胰腺癌微環(huán)境�。� 參考：《山西醫(yī)科大學》2011年碩士論文

【摘要】：目的: 初步研究胰腺癌微環(huán)境對樹突狀細胞(Dendritic cell，Dc)成熟的影響及功能變化并初步探討胰腺腫瘤細胞免疫逃逸的發(fā)生機制。 方法： 培養(yǎng)樹突狀細胞，加入粒細胞巨噬細胞集落刺激因子(GM-CSF)40ng/ml、白細胞介素4(IL-4)40ng/ml，培養(yǎng)到第６天時得到大量的未成熟樹突狀細胞(immaturedendriticcells,imDC)，，加入大鼠胰腺癌細胞（AR42J cell）培養(yǎng)上清液誘導共培養(yǎng)3天，流式細胞術檢測DC的表面分子CD86、CD80的表達（n=6），觀察能否延緩或阻斷imDC的成熟及其在脂多糖(lipopolysaccharide,LPS)刺激后這種作用能否被逆轉(zhuǎn)。并觀察AR42J細胞培養(yǎng)上清誘導的DC對同種異體混合淋巴細胞增殖情況。 結(jié)果： 加入AR42J細胞培養(yǎng)上清液誘導的DC與正常培養(yǎng)的DC比較，CD80+ CD86+陽性率分別為（7.15±0.71）%和（70.88±3.60）%，AR42J細胞培養(yǎng)上清誘導的DC經(jīng)LPS刺激后細胞的表面分子CD80+CD86+陽性率表達仍然較低（7.43±1.05）%，表明AR42J細胞培養(yǎng)上清液對imDC的成熟有阻斷作用。AR42J細胞培養(yǎng)上清液誘導的imDC組刺激同種異體混合淋巴細胞增殖的強度顯著低于正常培養(yǎng)的imDC組刺激同種異體混合淋巴細胞增殖的強度。 結(jié)論: 體外AR42J細胞培養(yǎng)上清液可以誘導imDC處于不成熟狀態(tài)，這種不成熟狀態(tài)不容易被逆轉(zhuǎn),并且這種被誘導的imDC與正常培養(yǎng)的imDC比較刺激同種異體混合淋巴細胞增殖的能力顯著降低。
[Abstract]:Objective: To study the effect of pancreatic cancer microenvironment on dendritic cell (DC) maturation and its functional changes, and to explore the mechanism of pancreatic tumor cell immune escape. Methods: Dendritic cells were cultured, then 40 ng / ml of granulocyte macrophage colony stimulating factor (GM-CSFN) and 40 ng / ml of interleukin-4 (IL-4) were added into the culture medium. On the 6th day, a large number of immature dendritic cells were obtained and cultured in the supernatant of rat pancreatic cancer cell line AR42J for 3 days. Flow cytometry was used to detect the expression of CD86 + CD80 on the surface of DC and to observe whether it could delay or block the maturation of imDC and whether it could be reversed after lipopolysaccharide (LPS) was stimulated by lipopolysaccharide. The proliferation of allogeneic mixed lymphocytes induced by supernatants of AR42J cells was observed. Results: The positive rates of CD80 CD86 were 7.15 鹵0.71% and 70.88 鹵3.60% in DC induced by AR42J cell culture supernatant and 70.88 鹵3.60% in normal DC, respectively. The CD80 CD86 positive rate of DC induced by LPS was still lower than that of DC cultured in normal culture. The supernatant of AR42J cell culture can block the maturation of imDC. The intensity of stimulating the proliferation of allogeneic mixed lymphocytes in imDC group induced by supernatant of AR42J cell culture is significantly lower than that in imDC group of normal culture. The intensity of cell proliferation. Conclusion: The supernatant of AR42J cell culture in vitro could induce imDC to be in immature state, which could not be easily reversed, and the ability of inducing imDC to stimulate the proliferation of allogeneic mixed lymphocytes was significantly lower than that of normal cultured imDC.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R392

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