本文選題：骨骼肌衛(wèi)星細(xì)胞 + 機(jī)械生長因子��； 參考：《上海體育學(xué)院》2011年碩士論文

【摘要】：MGF組:24h時(shí),15ng/ml組、25ng/ml組、35ng/ml組和45ng/ml組OD值均顯著高于對照組(P0.01,P0.01,P0.01,P0.05),55ng/ml組與對照組無顯著差異(P0.05)。48h時(shí),15ng/ml組、25ng/ml組、35ng/ml組OD值均顯著高于對照組(P0.05,P0.01,P0.01), 45ng/ml組、55ng/ml組與對照組之間無顯著差異(P0.05)。72h時(shí),15ng/ml、25ng/ml組、35ng/ml組OD值均顯著高于對照組(P0.01),45ng/ml組與對照組之間無顯著差異(P0.05)。而55ng/ml組OD值低于對照組(P0.05)。96h時(shí),15ng/ml、25ng/ml組、35ng/ml組與對照組之間差異沒有顯著性(P0.05)。 4. Transwell法測定細(xì)胞遷移的OD值顯示。 IGF-I組:15ng/ml組、35ng/ml組與對照組之間的無顯著差異(P0.05);25ng/ml、45ng/ml組、55ng/ml組OD值均顯著高于對照組(P0.01),其中25ng/ml組顯著高于45ng/ml組(P0.01),55ng/ml組與45ng/ml組之間的無顯著差異(P0.05)。 MGF組:15ng/ml組、35ng/ml組與對照組之間無顯著差異(P0.05);25ng/ml、45ng/ml組、55ng/ml組OD值顯著高于對照組(P0.01),其中45ng/ml組和55ng/ml組均顯著高于25ng/ml組(P0.01,P0.05)。 結(jié)論 1. IGF-I和MGF均可以促進(jìn)骨骼肌衛(wèi)星細(xì)胞增殖且呈濃度、時(shí)間依賴性,IGF-I最佳促增殖濃度為15ng/ml-35ng/ml , MGF最佳促增殖濃度為25ng/ml-35ng/ml,干預(yù)24h后即開始增殖。 2. IGF-I和MGF均可以促進(jìn)骨骼肌衛(wèi)星細(xì)胞遷移,IGF-I促遷移的最佳濃度為25ng/ml,MGF最佳濃度為45ng/ml-55ng/ml。MGF組:24h時(shí),15ng/ml組、25ng/ml組、35ng/ml組和45ng/ml組OD值均顯著高于對照組(P0.01,P0.01,P0.01,P0.05),55ng/ml組與對照組無顯著差異(P0.05)。48h時(shí),15ng/ml組、25ng/ml組、35ng/ml組OD值均顯著高于對照組(P0.05,P0.01,P0.01), 45ng/ml組、55ng/ml組與對照組之間無顯著差異(P0.05)。72h時(shí),15ng/ml、25ng/ml組、35ng/ml組OD值均顯著高于對照組(P0.01),45ng/ml組與對照組之間無顯著差異(P0.05)。而55ng/ml組OD值低于對照組(P0.05)。96h時(shí),15ng/ml、25ng/ml組、35ng/ml組與對照組之間差異沒有顯著性(P0.05)。 4. Transwell法測定細(xì)胞遷移的OD值顯示。 IGF-I組:15ng/ml組、35ng/ml組與對照組之間的無顯著差異(P0.05);25ng/ml、45ng/ml組、55ng/ml組OD值均顯著高于對照組(P0.01),其中25ng/ml組顯著高于45ng/ml組(P0.01),55ng/ml組與45ng/ml組之間的無顯著差異(P0.05)。 MGF組:15ng/ml組、35ng/ml組與對照組之間無顯著差異(P0.05);25ng/ml、45ng/ml組、55ng/ml組OD值顯著高于對照組(P0.01),其中45ng/ml組和55ng/ml組均顯著高于25ng/ml組(P0.01,P0.05)。 結(jié)論 1. IGF-I和MGF均可以促進(jìn)骨骼肌衛(wèi)星細(xì)胞增殖且呈濃度、時(shí)間依賴性,IGF-I最佳促增殖濃度為15ng/ml-35ng/ml , MGF最佳促增殖濃度為25ng/ml-35ng/ml,干預(yù)24h后即開始增殖。 2. IGF-I和MGF均可以促進(jìn)骨骼肌衛(wèi)星細(xì)胞遷移,IGF-I促遷移的最佳濃度為25ng/ml,MGF最佳濃度為45ng/ml-55ng/ml。
[Abstract]:The OD value of MGF group was significantly higher than that of control group P0.01ngpml group and 45ng/ml group at 24 hours. There was no significant difference between the control group and the control group. The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05ngP / ml group, P0.05g / ml group and control group, P0.05ng / ml group and control group at 2h / 48h). The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05g / ml group and control group). There was no significant difference between the 45ng/ml group and the control group (P0.05g / ml group and the control group at 2h. 7 h). The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05g / ml group and control group). The OD value of 15ng / ml 25ng / ml group was significantly higher than that of control group (P0.01ng / ml) and control group (P0.05ng-1 / ml), and there was no significant difference between control group and control group (P0.05ng / ml). However, the OD value of 55ng/ml group was significantly lower than that of control group (P 0.05) at 96 h. There was no significant difference between the 35 ng / ml group and the control group at the time of 15 ng / ml or 25 ng / ml. 4. OD value of cell migration was measured by Transwell method. There was no significant difference between the IGF-I group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). The OD value of the 25ng/ml group was significantly higher than that of the 45ng/ml group (P0.01ngr / ml group) and the 45ng/ml group (P0.05N). There was no significant difference between the MGF group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P 0.05ng / ml). The OD values of the 45ng/ml group and the 55ng/ml group were significantly higher than that of the 25ng/ml group (P0.01P0.05). Conclusion 1. Both IGF-I and MGF could promote the proliferation of skeletal muscle satellite cells. The best concentration of IGF-I was 15ng/ml-35ng/ml, and the best concentration of MGF was 25ng / ml-35ng / ml. After 24 hours of intervention, the proliferation began. 2. IGF-I and MGF can promote the migration of skeletal muscle satellite cells. The best concentration of IGF-I to promote migration of skeletal muscle satellite cells is 25ng / ml MGF at 24 hours in 45ng/ml-55ng/ml.MGF group. The OD value of 25ng / ml group and 45ng/ml group is significantly higher than that of control group P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.05ngP / ml at 48h. There is no significant difference between control group and control group (P0.05g / ml, 48h). The OD value of the 35ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). There was no significant difference between the 45ng/ml group and the control group (P0.05g / ml). There was no significant difference between the 45ng/ml group and the control group at 72h. The OD value of the 35ng / ml group was significantly higher than that of the control group (P0.01ngml group and control group). There was no significant difference between the control group and the control group. However, the OD value of 55ng/ml group was significantly lower than that of control group (P 0.05) at 96 h. There was no significant difference between the 35 ng / ml group and the control group at the time of 15 ng / ml or 25 ng / ml. 4. OD value of cell migration was measured by Transwell method. There was no significant difference between the IGF-I group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). The OD value of the 25ng/ml group was significantly higher than that of the 45ng/ml group (P0.01ngr / ml group) and the 45ng/ml group (P0.05N). There was no significant difference between the MGF group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P 0.05ng / ml). The OD values of the 45ng/ml group and the 55ng/ml group were significantly higher than that of the 25ng/ml group (P0.01P0.05). Conclusion 1. Both IGF-I and MGF could promote the proliferation of skeletal muscle satellite cells. The best concentration of IGF-I was 15ng/ml-35ng/ml, and the best concentration of MGF was 25ng / ml-35ng / ml. After 24 hours of intervention, the proliferation began. 2. Both IGF-I and MGF could promote the migration of skeletal muscle satellite cells. The best concentration of IGF-I was 25ng / ml ml-55ng / ml.
【學(xué)位授予單位】：上海體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R329

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 葉雷雷;力竭運(yùn)動(dòng)及鈍挫傷對大鼠骨骼肌衛(wèi)星細(xì)胞及肝細(xì)胞生長因子的影響[D];揚(yáng)州大學(xué);2012年

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本文編號：1936053