本文選題：人呼吸道合胞病毒(HRSV) + G蛋白　； 參考：《病毒學報》2017年06期

【摘要】：對2008~2014年收集的中國8省市人呼吸道合胞病毒(Human respiratory syncytial virus,HRSV)A亞型87株代表株進行G蛋白編碼基因全長核苷酸序列測定和分析,闡明其核苷酸和氨基酸動態(tài)變異特征。根據(jù)G蛋白第二高變區(qū)的靶基因特征,從2008~2014年中國8省市流行的A亞型人呼吸道合胞病毒中挑選代表株,并采用RT-PCR方法對G蛋白編碼基因進行擴增和序列測定,通過Sequencher 5.0、MEGA5.05等生物信息學軟件進行序列拼接、比對,分析基因親緣性和氨基酸變異特點。2008~2014年中國8個代表省市收集的296份HRSVA陽性標本,依據(jù)靶基因變異程度及特征共挑選出代表株87株,覆蓋2008~2014年流行的各基因型,包括GA5,NA4,NA1,NA3,ON1;對87株代表株進行G蛋白編碼基因全長序列測定和同源性分析,核苷酸同源性為88.21%~100%,氨基酸同源性為82.09%~100%,與原型株long株的核苷酸和氨基酸同源性分別為89.22%~91.07%和82.77%~86.82%;變異主要集中在第二高變區(qū),其次在胞漿區(qū)和第一高變區(qū)。根據(jù)G蛋白全長核苷酸序列構(gòu)建親緣關系進化樹,87株代表株可以分為5個分支,與國際上通用的依據(jù)G蛋白第二高變區(qū)為靶基因進行的基因分型及進化樹分支結(jié)果一致。本研究系統(tǒng)分析了2008~2014年中國8省市人呼吸道合胞病毒A亞型G蛋白編碼基因的基因特征及氨基酸變異情況,為中國人呼吸道合胞病毒的病原學研究,預防和控制提供了重要的病毒學基礎研究數(shù)據(jù)。
[Abstract]:The full-length nucleotide sequence of G protein encoding gene of 87 representative strains of human respiratory syncytial virus (HRRSV) from 2008 to 2014 in 8 provinces and cities of China were determined and analyzed, and the dynamic variation characteristics of nucleotide and amino acid were elucidated. According to the target gene characteristics of the second high variable region of G protein, the representative strains of human respiratory syncytial virus A subtype A were selected from 8 provinces and cities of China from 2008 to 2014. The G protein encoding gene was amplified and sequenced by RT-PCR. Sequencher 5.0 MEGA 5.05 and other bioinformatics software were used to sequence splice and compare the genetic relationship and amino acid variation characteristics. From 2008 to 2014, 296 HRSVA positive specimens were collected from 8 representative provinces and cities in China. A total of 87 representative strains were selected according to the degree and characteristics of target gene variation, covering all the prevalent genotypes from 2008 to 2014, including GA5, NA4, NA1, NA1, NA3ON1.The full-length sequence and homology analysis of G-protein coding genes were performed on 87 representative strains. The homology of nucleotide and amino acid was 88.21 and 82.09100, respectively, and the homology of nucleotide and amino acid with long strain was 89.222.22% and 82.77%, respectively. The variation was mainly concentrated in the second high variable region, followed by the cytoplasmic region and the first high variable region. According to the full-length nucleotide sequence of G protein, 87 representative strains of phylogenetic tree can be divided into 5 branches, which are consistent with the results of the universal genotyping and phylogenetic tree branches based on the target gene of the second high variable region of G protein. This study systematically analyzed the gene characteristics and amino acid variation of subtype A G protein encoding gene of human respiratory syncytial virus A in 8 provinces and cities of China from 2008 to 2014, which is the etiological study of Chinese respiratory syncytial virus. Prevention and control provide important basic virology research data.
【作者單位】： 中國疾病預防控制中心病毒病預防控制所衛(wèi)生部醫(yī)學病毒學和病毒病重點實驗室世界衛(wèi)生組織西太平洋區(qū)麻疹/風疹參比實驗室;北京市大興區(qū)鹿圈社區(qū)衛(wèi)生服務中心;廣州醫(yī)科大學第一附屬醫(yī)院呼吸疾病國家重點實驗室廣州呼吸疾病研究所;長春市兒童醫(yī)院;甘肅省疾病預防控制中心;陜西省疾病預防控制中心;湖南省疾病預防控制中心;安徽理工大學醫(yī)學院;
【基金】：國家自然科學基金(項目號:81772195),題目:我國大陸流行的人呼吸道合胞病毒F蛋白抗原位點動態(tài)變異研究 國家重大科技專項課題(項目號:2013ZX10004202),題目:艾滋病和病毒性肝炎等重大傳染病防治~~
【分類號】：R373

【相似文獻】

相關博士學位論文 前1條

1 郭野;G蛋白β2亞基調(diào)控新皮質(zhì)神經(jīng)元多極向雙極形態(tài)轉(zhuǎn)換[D];東北師范大學;2017年

，

本文編號：1934810