本文選題：股骨頭壞死 + 脂多糖類　； 參考：《中國組織工程研究》2017年28期

【摘要】：背景:前期研究采用激素聯(lián)合脂多糖已成功構建股骨頭缺血壞死兔模型。目的:進一步觀察激素性股骨頭壞死早期骨質的動態(tài)變化。方法:成年新西蘭大白兔24只,隨機分為實驗組(n=12)和對照組(n=12)。實驗組采用激素聯(lián)合脂多糖改良技術建立激素性股骨頭缺血壞死兔模型,對照組相同部位注射等體積生理鹽水,飼養(yǎng)條件不變。建模完成后,分別于第5,10,15,20天進行股骨頭多層螺旋CT、顯微CT及蘇木精-伊紅染色檢測,觀察股骨頭結構,骨小梁形態(tài)、分布,測定骨質密度及空骨陷窩率。結果與結論:(1)影像學結果顯示,實驗組與對照組兔股骨頭形態(tài)完整,關節(jié)面光滑,第15,20天實驗組股骨頭骨皮質變薄,骨小梁分布稀疏,間距增寬,不連續(xù),骨礦物質密度、組織礦物質密度、骨體積分數值均顯著低于對照組(P0.01);(2)病理學結果顯示,實驗組空骨陷窩和脂肪細胞不斷增多,骨細胞和造血細胞逐漸減少,第15,20天空骨陷窩百分率顯著高于對照組(P0.01);(3)結果說明,激素性股骨頭壞死早期股骨頭內骨細胞壞死增多,伴骨小梁微細骨折發(fā)生,導致骨質密度減低,最終可造成骨重塑障礙。
[Abstract]:Background: a rabbit model of avascular necrosis of femoral head has been successfully constructed by hormone and lipopolysaccharide. Objective: to observe the dynamic changes of bone in early stage of steroid-induced femoral head necrosis. Methods: 24 adult New Zealand white rabbits were randomly divided into experimental group (n = 12) and control group (n = 12). The model of steroid-induced avascular necrosis of femoral head was established by hormone and lipopolysaccharide in the experimental group, and the same volume of saline was injected into the control group. After modeling, the multislice spiral CTs of femoral head, microscopic CT and hematoxylin eosin staining were performed on day 5, 10, 15 and 20, respectively. The structure of femoral head, the shape and distribution of trabecular bone, bone density and the rate of empty bone lacuna were observed. Results and conclusion the imaging results showed that the femoral head of the experimental group and the control group had a complete shape and smooth articular surface. On day 1520, the femoral head of the experimental group became thin, the bone trabecular distribution was sparse, the spacing widened, the bone mineral density was discontinuous. The tissue mineral density and bone volume fraction were significantly lower than those in the control group (P 0.01). The pathological results showed that the number of empty bone lacunae and adipocytes in the experimental group was increasing and the number of bone cells and hematopoietic cells was decreasing. The results showed that osteonecrosis of femoral head increased in the early stage of steroid-induced osteonecrosis, accompanied by bone trabecular microfracture, which resulted in the decrease of bone density, which resulted in bone remodeling disorder.
【作者單位】： 德陽市人民醫(yī)院放射科;成都醫(yī)學院第一附屬醫(yī)院放射科;
【基金】：四川省教育廳資助項目(15ZB0243)~~
【分類號】：R-332;R681.8

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