發(fā)布時(shí)間：2018-05-18 00:02

  本文選題：單核細(xì)胞增生李斯特菌 + rmlB��； 參考：《微生物學(xué)通報(bào)》2017年01期

【摘要】：【目的】探究單核細(xì)胞增生李斯特菌(Listeria monocytogenes,Lm)rmlB基因在細(xì)菌耐藥、生物被膜形成和毒力方面的作用�！痉椒ā客ㄟ^同源重組的方法敲除Lm染色體上的rmlB基因,比較野生株與rmlB缺失株在耐藥性方面的差異;利用微孔板法觀測(cè)rmlB缺失菌株生物被膜形成能力的變化;利用RT-PCR檢測(cè)缺失菌株中主要毒力基因轉(zhuǎn)錄表達(dá),并觀察rmlB缺失對(duì)細(xì)菌溶血活性的影響�！窘Y(jié)果】同野生菌株相比,rmlB缺失菌株對(duì)頭孢菌素和桿菌肽等作用位點(diǎn)在細(xì)菌細(xì)胞壁和細(xì)胞膜的敏感性顯著增加(P≤0.01),生物被膜形成能力顯著降低(P≤0.01),細(xì)菌主要毒力基因hly的轉(zhuǎn)錄表達(dá)及溶血活性也發(fā)生顯著降低(P≤0.01)�！窘Y(jié)論】rmlB基因在Lm生物被膜形成和耐受作用位點(diǎn)位于細(xì)胞壁和細(xì)胞膜的抗生素及細(xì)菌毒力方面具有重要作用。
[Abstract]:[objective] to investigate the role of Listeria monocytogenes B gene in bacterial drug resistance, biofilm formation and virulence. [methods] the rmlB gene on LM chromosome was knockout by homologous recombination. The differences of drug resistance between wild strain and rmlB deletion strain were compared, the changes of biofilm forming ability of rmlB deletion strain were observed by microplate method, and the transcription expression of main virulence genes in deletion strain was detected by RT-PCR. The effect of rmlB deletion on the hemolytic activity of bacteria was observed. [results] compared with wild strain, rmlB deletion strain was significantly more sensitive to cephalosporin and bacitracin in the cell wall and cell membrane of bacteria than wild strain (P 鈮，

本文編號(hào)：1903454