發(fā)布時間：2018-05-17 23:14

  本文選題：受體 + 生長素　； 參考：《吉林農業(yè)大學》2011年碩士論文

【摘要】：生長素是植物生長過程中所必需的一種植物激素,在植物激素中是最早被發(fā)現的,存在于植物界的所有植物中,并在植物生長過程中起重要的協同作用。在植物生理活動過程中扮演重要角色。生長素主要產生于植物的分生組織——根尖和莖尖中,其他部位——莖和葉也可少量產生。它影響植物的生長進程,如細胞分裂和伸長,葉片及根、花、果實等形成,影響植物的向性運動。最近的二十年,以模式植物擬南芥為材料,科學家們主要從生長素的受體這個方面進行加進研究。生長素受體的特性和生長素的感應機制是在生長素發(fā)現的一個世紀以來被熱切關注的課題。在1980年,生長素結合植物蛋白的定位,顯示了受體成員中包含有生長素結合蛋白Auxin Binding Proteinl (ABP1),無疑影響了生長素的分子應答。2005年,由Mark Estelle和Ottoline Leyser兩個研究小組在多年研究工作的基礎上,發(fā)現TIR1在IAA應答基因的表達調控中起作用,能構成SCFTIR1復合體的F-box蛋白。TIR1已被國際學術界確定為生長素的受體。AXR1蛋白參與生長素信號傳導的調節(jié),在學術上研究及報道較少,屬生長素受體研究的最新內容。 人參細胞生長素的受體基因至今未見研究文獻報道,本實驗主要根據模式植物擬南芥已知的生長素受體基因TIR1、ABP1的保守序列,設計人參生長素受體基因的引物,克隆人參生長素受體基因；后將重組的目的基因轉移到到穿梭載體PINT-HIS3NB上,并在大腸桿菌DH10B上進行表達,得到陽性單克隆菌株后,提取質粒,進行測序比對,確定人參生長素受體TIR1、ABP1的基因序列。研究通過對生長素受體ABP1和TIR1在人參中的基因克隆分析,目的是闡明人參中生長素的受體基因序列以及ABP1和TIR1受體在人參中的序列分布,為進一步研究人參生長素的信號傳遞模式及對人參生長生理活動的影響機理奠定研究基礎。
[Abstract]:Auxin is an essential plant hormone in plant growth. It is first found in plant hormones and exists in all plants in the plant kingdom and plays an important synergistic role in plant growth. Play an important role in plant physiological activities. Auxin is mainly produced in the meristem and stem tip of plant, and in other parts-stem and leaf. It affects the growth process of plants, such as cell division and elongation, leaf and root, flower, fruit and so on, and affects the directional movement of plants. In the last two decades, using model plant Arabidopsis thaliana as a material, scientists have been studying auxin receptors. The properties of auxin receptors and the sensing mechanism of auxin have been paid close attention to since the discovery of auxin in a century. In 1980, the localization of auxin binding plant proteins showed that the receptor member contained the auxin binding protein Auxin Binding Proteinl / ABP1, which undoubtedly affected the molecular response of auxin. Based on years of research work by Mark Estelle and Ottoline Leyser, we found that TIR1 plays an important role in the regulation of IAA response gene expression. F-box protein. TIR1, which can form SCFTIR1 complex, has been identified as auxin receptor. AXR1 protein is involved in the regulation of auxin signal transduction. The receptor gene of Ginseng cell auxin has not been reported so far. The primer of the receptor gene of Ginseng auxin receptor was designed based on the conserved sequence of the known auxin receptor gene TIR1 / ABP1 in Arabidopsis thaliana. The recombinant target gene was transferred to the shuttle vector PINT-HIS3NB and expressed on the Escherichia coli DH10B. After obtaining the positive monoclonal strain, the plasmid was extracted and sequenced. The sequence of ABP1 gene of Ginseng auxin receptor TIR1 was identified. The aim of this study was to elucidate the sequence of auxin receptor genes and the distribution of ABP1 and TIR1 receptors in ginseng by cloning and analyzing the auxin receptor ABP1 and TIR1 in ginseng. The results provide a basis for further study on the signal transduction mode of Ginseng auxin and the mechanism of its effect on the growth and physiological activities of Panax ginseng.
【學位授予單位】：吉林農業(yè)大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R346

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