本文選題：人血清白蛋白 + 孔雀石綠��； 參考：《蘭州大學(xué)》2012年碩士論文

【摘要】：人血清白蛋白(human serum albumin, HSA)是血漿中含量最多的一種蛋白質(zhì),和其它蛋白質(zhì)相比,HSA具有分子量小、溶解性大、穩(wěn)定性較好、很好的親和性及能同多種物質(zhì)結(jié)合(如金屬離子、脂肪酸、氨基酸、激素及藥物等)的特點(diǎn)。通過(guò)研究物質(zhì)和HSA的相互作用,就可以得到這些物質(zhì)在生物體內(nèi)被轉(zhuǎn)運(yùn)、分配和代謝過(guò)程中的重要信息。因此,對(duì)金屬離子、有機(jī)小分子與HSA相互作用的研究是很有意義的,該課題已經(jīng)成為生命科學(xué)、化學(xué)及臨床醫(yī)學(xué)研究的重要課題之一。 本論文除了研究分子或離子與HSA的相互作用之外,還對(duì)熒光碳納米顆粒(CNPs)的性質(zhì)進(jìn)行了研究。由于碳是自然界中含量最豐富的元素之一,且碳材料的毒性都非常低,所以碳納米材料一直是科技創(chuàng)新的前沿領(lǐng)域。 本論文在前人研究的基礎(chǔ)上,開展了以下三個(gè)方面的工作： 1.在模擬生理?xiàng)l件下,研究了孔雀石綠與HSA的相互作用。利用熒光光譜、圓二色譜和傅里葉變換紅外光譜技術(shù)確定了它們之間相互作用的結(jié)合常數(shù)、主要作用力以及孔雀石綠對(duì)HSA二級(jí)結(jié)構(gòu)的影響。利用分子模擬技術(shù)得到的熱力學(xué)參數(shù),確定了二者間的相互作用力。實(shí)驗(yàn)結(jié)果表明孔雀石綠可以使HSA發(fā)生熒光猝滅,猝滅機(jī)理為靜態(tài)猝滅。它們之間的結(jié)合常數(shù)KSV=2.9×104Lmol-1,結(jié)合位點(diǎn)數(shù)n=1.2。 2.在模擬生理?xiàng)l件下,利用熒光光譜和紫外光譜的方法研究了金屬離子Mo(Ⅵ)與HSA的相互作用。實(shí)驗(yàn)結(jié)果表明Mo(Ⅵ)可以使HSA發(fā)生熒光猝滅,猝滅機(jī)理為靜態(tài)猝滅。它們之間的結(jié)合常數(shù)KSV=1.29×104Lmol-1,結(jié)合位點(diǎn)數(shù)n=1.01。 3.利用硝酸氧化煤油灰的方法制得CNPs,它具有很好的水溶性,在水溶液中可以穩(wěn)定存在三個(gè)月以上且其熒光強(qiáng)度不會(huì)降低。通過(guò)分析CNPs的掃描電鏡圖可知CNPs的平均粒徑約為50nm左右。為了提高它的性能,用硫脲(TC)對(duì)其表面進(jìn)行了修飾,修飾后的CNPs (CNPs-TC)的熒光性質(zhì)發(fā)生了一定的變化,而且CNPs-TC與CNPs的熒光強(qiáng)度對(duì)pH值變化的反應(yīng)不同。此外,我們還研究了CNPs、CNPs-TC與金屬離子的相互吸附作用。結(jié)果表明CNPs-TC對(duì)金屬離子的吸附和沉降作用更為明顯。
[Abstract]:Human serum albumin (HSA) is the most abundant protein in plasma. Compared with other proteins, HSA has the characteristics of small molecular weight, large solubility, good stability, good affinity and ability to combine with a variety of substances (such as metal ions, fatty acids, amino acids, hormones and drugs, etc.). Through the study of substances and HSA The interaction of these substances can be important information in the process of transport, distribution and metabolism of these substances in organisms. Therefore, it is very meaningful to study the interaction of metal ions and small organic molecules with HSA. The subject has become one of the important topics in the research of life science, chemistry and clinical medicine.
In addition to the study of the interaction of molecules or ions with HSA, the properties of fluorescent carbon nanoparticles (CNPs) are also studied. Since carbon is one of the most abundant elements in nature and the toxicity of carbon materials is very low, carbon nanomaterials have always been the frontiers of science and technology innovation.
On the basis of previous studies, this thesis has carried out the following three aspects:
1. the interaction between malachite green and HSA was studied under simulated physiological conditions. Using fluorescence spectroscopy, circular two chromatography and Fu Liye transform infrared spectroscopy, the interaction constants, main forces and the effect of malachite green on the structure of HSA two were determined. The interaction force between the two is determined. The experimental results show that the malachite green can cause the fluorescence quenching of HSA, and the quenching mechanism is static quenching. The binding constant between them is KSV=2.9 x 104Lmol-1, and the number of binding sites is n=1.2.
2. under simulated physiological conditions, the interaction between metal ions Mo (VI) and HSA was studied by fluorescence and UV spectra. The experimental results show that Mo (VI) can cause fluorescence quenching of HSA and the quenching mechanism is static quenching. The binding constant between them is KSV=1.29 * 104Lmol-1, and the number of bits n=1.01. is combined.
3. the CNPs is prepared by oxidation of kerosene ash with nitric acid. It has good water solubility. It can be stable in aqueous solution for more than three months and its fluorescence intensity will not decrease. By analyzing the scanning electron microscopy of CNPs, the average particle size of CNPs is about 50nm. In order to improve its performance, the surface of it is modified with thiourea (TC). The fluorescence properties of the modified CNPs (CNPs-TC) have changed a certain amount, and the reaction of the fluorescence intensity of CNPs-TC and CNPs to the change of pH value is different. In addition, we also studied the interaction between CNPs, CNPs-TC and metal ions. The results show that CNPs-TC has more obvious effect on the absorption and settlement of metal ions.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R341

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 趙長(zhǎng)容;方芳;陳慧;連國(guó)軍;曹建明;;熒光光譜法研究偶氮胂Ⅲ與牛血清白蛋白的相互作用[J];廣東微量元素科學(xué);2013年06期

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本文編號(hào)：1902203