本文選題：慢性胃炎 + 動物模型��； 參考：《遼寧中醫(yī)藥大學》2011年碩士論文

【摘要】：目的: 1、探討胃炎大鼠脾胃虛證、濕熱證模型的造模方法 2、探討不同證型胃炎大鼠在能量代謝方面的指標差異 材料和方法: 選取40只清潔級SD大鼠,隨機分成四組,脾胃濕熱組,脾胃虛弱組,模型組,正常組。造模期間觀察大鼠的毛色、食量、體重的變化。造模四周后,濕熱組8只,脾胃虛弱組9只,模型組、正常組各10只。取胃黏膜和骨骼肌通過酶促反應和定磷方法,檢測各個組別能量代謝指標ATP酶活性,比較相關性。取胃竇部光鏡下通過HE染色,分析病理結果。 結果: 骨骼肌和胃黏膜ATP酶活性測試表明:脾胃濕熱組與正常組相比,P0.05,有統(tǒng)計學差異。脾胃虛弱組與正常組相比,P0.05,有統(tǒng)計學差異。模型組與正常組相比,P0.05,有統(tǒng)計學差異。 胃竇部HE病理染色顯示:正常組:大鼠黏膜上皮細胞排列整齊,腺體形態(tài)規(guī)則,大小一致,固有層偶見炎性細胞浸潤。�？战M:胃黏膜血管擴張充血,腺體形態(tài)尚規(guī)則,但固有層可見少量炎癥細胞浸潤。提示已有慢性胃炎的病理學改變。濕熱組:胃黏膜缺損,脫落,壞死,固有層見大量炎癥細胞浸潤。脾虛組:胃黏膜缺損,脫落,壞死,固有層可見炎癥細胞較濕熱少一些。 結論: 脾胃虛弱組數(shù)值明顯偏低,表明處于長期處于脾胃虛弱狀態(tài)的大鼠ATP合成減少,機體能量代謝減少。脾胃濕熱組數(shù)值明顯偏高,表明基礎酶活力和能量代謝增高。胃竇部病理染色顯示:各組炎癥程度,脾胃是熱組脾胃虛弱組模型組正常組。
[Abstract]:Objective: 1. To explore the method of making model of spleen and stomach deficiency syndrome and damp-heat syndrome in rats with gastritis. 2. To explore the difference of energy metabolism in rats with different types of gastritis. Materials and methods: Forty SD rats of clean grade were randomly divided into four groups: spleen and stomach damp-heat group, spleen and stomach weakness group, model group and normal group. The changes of color, food intake and body weight of the rats were observed during modeling. Four weeks later, there were 8 rats in damp-heat group, 9 in spleen and stomach weakness group, 10 in model group and 10 in normal group. Gastric mucosa and skeletal muscle were measured by enzymatic reaction and phosphorus fixation to detect the activity of ATP enzyme in each group of energy metabolism indexes. The pathological results of gastric antrum were analyzed by HE staining under light microscope. Results: The activity of ATP enzyme in skeletal muscle and gastric mucosa showed that there was significant difference between the spleen and stomach dampness heat group and the normal group (P 0.05). Compared with the normal group, the spleen and stomach weak group had significant difference (P 0.05). There was statistical difference between model group and normal group (P 0.05). The HE staining of gastric antrum showed that in normal group, the epithelial cells were arranged neatly, the glands were regular, the size was the same, and inflammatory cell infiltration was occasionally seen in the lamina propria. Model empty group: gastric mucosal vasodilation hyperemia, glandular shape is regular, but there are a few inflammatory cells infiltration in lamina propria. It suggests that there are pathological changes in chronic gastritis. Damp-heat group: gastric mucosal defect, abscission, necrosis, lamina propria a large number of inflammatory cell infiltration. Spleen deficiency group: gastric mucosal defect, shedding, necrosis, inflammatory cells visible in the lamina propria less than damp and heat. Conclusion: The value of spleen and stomach weakness group was significantly lower, which indicated that ATP synthesis and energy metabolism were decreased in rats with chronic weakness of spleen and stomach. The value of spleen and stomach damp-heat group was significantly higher than that of the control group, indicating that the activity of basic enzymes and energy metabolism were increased. The pathological staining of gastric antrum showed that the degree of inflammation in each group and spleen and stomach were normal group.
【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R-332

【參考文獻】

相關期刊論文 前10條

1 程方平;劉松林;李云海;周璇;梅國強;;溫病濕熱證大鼠模型肝線粒體K~+-Na~+-ATP酶活力變化的對比研究[J];浙江中醫(yī)藥大學學報;2008年02期

2 陳麗萍;吳山力;Fehēr Erzsēbet;Altdorfer Károly;遲寶榮;;碘乙酰胺誘導的大鼠胃炎中神經(jīng)肽免疫陽性神經(jīng)纖維的變化[J];吉林大學學報(醫(yī)學版);2007年01期

3 高碧珍,黃愛民,章志安,李燦東,詹子銘,林雪娟;不同證候模型大鼠胃黏膜細胞凋亡與證的相關性研究[J];中華中醫(yī)藥雜志;2005年09期

4 黃秀深,趙燕,張力華,賈波;中焦?jié)褡枳C大鼠紅細胞膜Na~+-K~+ATP酶活性研究[J];成都中醫(yī)藥大學學報;2000年04期

5 楊春波,柯曉,李秀娟,傅肖巖,祁建生,楊永f;脾胃濕熱證的臨床研究[J];福建中醫(yī)學院學報;1999年04期

6 楊春波;;脾胃濕熱理論的形成及臨床應用[J];福建中醫(yī)藥大學學報;2010年05期

7 王學民,王滿,燕穎軍;三仁湯加減治療脾胃濕熱型慢性胃炎89例[J];河北醫(yī)藥;2002年11期

8 鄒世潔,陳小野,佟彤,王震,徐世杰;大鼠胃炎證病結合模型舌象組織病理觀察[J];湖北中醫(yī)雜志;2002年03期

9 呂文亮,程方平,黃廷榮;燥濕運脾湯對脾胃濕熱中阻證作用的實驗研究[J];湖北中醫(yī)雜志;2002年04期

10 張淑芹,趙林山,鄭繼奎,楊永良,郭立波;慢性萎縮性胃炎動物模型的復制[J];哈爾濱師范大學自然科學學報;2001年06期

，

本文編號：1888795