本文選題：肥胖癥 + 哈薩克族�。� 參考：《中華臨床醫(yī)師雜志(電子版)》2016年04期

【摘要】：目的利用無菌小鼠建立新疆哈薩克族肥胖兒童菌群人源化(HFA)動物模型,研究哈薩克族肥胖兒童的腸道菌群在無菌小鼠體內(nèi)的定植規(guī)律,為深入研究哈薩克族兒童肥胖的病理生理機制提供了一種新的選擇方式。方法收集新疆哈薩克族肥胖兒童新鮮糞便樣本,選取20只無菌級C57BL/6J小鼠(雌雄各半),于無菌小鼠3周齡時,開始灌胃接種哈薩克族肥胖兒童糞便菌懸液,接種1個月后收集HFA小鼠新鮮糞便樣本,利用PCR-DGGE技術(shù)進行檢測,對DGGE圖譜進行相似性UPGMA聚類分析,評價哈薩克族肥胖兒童腸道菌群在HFA小鼠體內(nèi)的定植規(guī)律。結(jié)果 HFA小鼠的菌群平均豐富度S為哈薩克族肥胖兒童的0.76倍;HFA小鼠的菌群平均均勻度E及多樣性指數(shù)H均低于哈薩克族肥胖兒童,但差異無明顯統(tǒng)計學(xué)意義;HFA小鼠與哈薩克族肥胖兒童腸道菌群的總相似度為64%,而HFA小鼠之間相似度為73%~94%;大部分雌性HFA小鼠與雄性HFA小鼠在聚類分析圖上分離。結(jié)論 (1)本實驗成功利用無菌C57BL/6J小鼠模擬哈薩克族肥胖兒童的特征性腸道菌群環(huán)境;(2)該HFA動物模型是探索哈薩克族兒童特征性肥胖與腸道菌群關(guān)系的一種新的選擇方式。
[Abstract]:Objective to establish a humanized HFA-animal model of obese children of Kazak nationality in Xinjiang by using sterile mice and to study the colonization of intestinal flora of obese children of Kazak nationality in sterile mice. It provides a new way to study the pathophysiological mechanism of Kazak children obesity. Methods fresh fecal samples of obese Kazakh children in Xinjiang were collected, and 20 sterile C57BL/6J mice (half male and half female) were selected and inoculated with fecal bacteria suspension of obese Kazakh children at the age of 3 weeks. Fresh stool samples of HFA mice were collected one month after inoculation and detected by PCR-DGGE technique. The similarity UPGMA cluster analysis of DGGE profiles was performed to evaluate the colonization of intestinal flora of Kazakh obese children in HFA mice. Results the average abundance of bacteria in HFA mice was 0.76 times of that in obese Kazakh children. The average homogeneity E and diversity index of HFA mice were lower than those of obese Kazakh children. However, there was no significant difference between HFA-mice and Kazakh obese children in intestinal microflora. The total similarity between HFA-mice and Kazak obese children was 64, while that of HFA mice was 730.The majority of female HFA mice and male HFA mice were separated from male HFA mice by cluster analysis. Conclusion 1) C57BL/6J mice were successfully used to simulate the characteristic intestinal microflora environment of obese Kazakh children. The HFA animal model is a new way to explore the relationship between characteristic obesity and intestinal flora in Kazakh children.
【作者單位】： 新疆醫(yī)科大學(xué);新疆醫(yī)科大學(xué)第一附屬醫(yī)院兒科;新疆維吾爾自治區(qū)循證醫(yī)學(xué)研究所;
【基金】：國家自然科學(xué)基金(81460165) 新疆維吾爾自治區(qū)自然基金(2014211C056)
【分類號】：R723.14;R-332

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1 馮承蕓;肥胖兒童的生理及心理衛(wèi)生[J];中國校醫(yī);2001年05期

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