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  本文選題：山楂 + 果蠅�。� 參考：《天津科技大學(xué)》2011年碩士論文

【摘要】：山楂為薔薇科落葉灌木或小喬木,全國各地均有栽培,為藥食同源植物。山楂果中含有黃酮類、熊果酸、皂苷、糖類、有機酸類、色素等多種活性成分。這些化合物在心血管的治療與保健方面以及抗氧化等方面越來越受到人們的關(guān)注。 果蠅是較為普遍的研究抗衰老模型之一,可用于研究衰老對基因的影響與作用。已有研究證明,果蠅的衰老基因與人類的衰老基因高度相似。 目前,果蠅已被廣泛應(yīng)用于國內(nèi)外的抗衰老研究實驗中,但是應(yīng)用于山楂的抗衰老研究鮮見報道。本文以山楂醇提物為原料,以果蠅為動物模型,對山楂的抗衰老作用進行評價,探索研究山楂醇提物的體內(nèi)抗氧化性。旨在進一步研究探索山楂的體內(nèi)抗氧化、抗衰老作用,為山楂抗衰老作用的研究與開發(fā)提供理論依據(jù)。 第一,檢測山楂提取物中抗氧化物質(zhì)的種類與含量。以蘆丁為標準品,通過紫外分光光度法,測得總黃酮含量為58.60%：經(jīng)高效液相色譜法,測得原花青素B2、表兒茶素、金絲桃苷三種物質(zhì)的含量分別為12.72%、7.34%、3.31%。 第二,進行體內(nèi)抗氧化活性的研究。建立正常果蠅模型,分別飼喂不同劑量的山楂醇提物。各組相比較,飼喂山楂醇提物的果蠅較空白組壽命延長；總SOD、 CuZn-SOD、CAT酶活力均顯著提高(P0.01),且在一定范圍內(nèi)隨劑量增大活力增強,超出此范圍,上述作用降低；在一定濃度范圍內(nèi)MDA含量降低,各組之間呈現(xiàn)量效關(guān)系,濃度超過此范圍,作用減弱；不同劑量組之間也呈現(xiàn)差異性顯著。 建立高脂模型。飼喂不同劑量的山楂醇提物,隨劑量的增大壽命延長；總SOD、 CuZn-SOD、CAT酶活力均顯著提高(P0.01),且在一定范圍內(nèi)隨劑量增大活力增強,超出此范圍,作用降低；在一定濃度范圍內(nèi)MDA含量降低,各組之間呈現(xiàn)量效關(guān)系,濃度超過此范圍,作用減弱；不同劑量組之間也呈現(xiàn)差異性顯著。 建立急性致死模型。分別用雙氧水、Paraquat作用于已飼喂不同劑量山楂醇提物的果蠅,誘導(dǎo)其體內(nèi)的羥自由基與超氧陰離子氧化,經(jīng)壽命實驗驗證,高劑量組的果蠅壽命高于低劑量組。 經(jīng)上述實驗證明,山楂提取物具有較強的體內(nèi)抗氧化性,可開發(fā)抗衰老類保健食品。
[Abstract]:Hawthorn is a deciduous bush or small tree of Rosaceae. Hawthorn fruit contains flavonoids, ursolic acid, saponins, carbohydrates, organic acids, pigments and other active ingredients. More and more attention has been paid to these compounds in cardiovascular treatment and health care as well as antioxidant. Drosophila is one of the most popular anti-aging models, which can be used to study the effect of senescence on genes. Studies have shown that the aging genes of Drosophila are highly similar to those of humans. At present, Drosophila has been widely used in anti-aging experiments at home and abroad, but the anti-aging research on Hawthorn is rarely reported. In this paper, the anti-aging effect of Hawthorn was evaluated by using Hawthorn alcohol extract as raw material and Drosophila as animal model, and the antioxidation of Hawthorn alcohol extract in vivo was studied. The aim of this study was to explore the antioxidation and anti-aging effects of Hawthorn in vivo and to provide theoretical basis for the research and development of anti-aging effect of Hawthorn. First, the kinds and contents of antioxidants in Hawthorn extracts were detected. The content of total flavonoids was 58.60 by UV spectrophotometry, and the contents of proanthocyanidin B _ 2, epigallocatechin and hyperoside were 12.727.34cm and 3.31 respectively by HPLC. Second, the study of antioxidant activity in vivo. A normal Drosophila model was established and fed with different doses of Hawthorn alcohol extract. Compared with the control group, Drosophila melanogaster fed with Hawthorn alcohol extract had longer life span, total SOD, CuZn-SOD cat enzyme activity increased significantly, and the activity increased with the increase of dosage, beyond this range, the above effects decreased. In a certain concentration range, the content of MDA decreased, there was a dose-effect relationship between the groups, the concentration exceeded this range, the effect was weakened, and there were significant differences among different dose groups. Establish high fat model. The activity of total SOD and CuZn-SODX cat increased significantly with the increase of dose, and the activity of total SOD and CuZn-SODX cat increased with the increase of dose, and the activity increased with the increase of dose, and the effect decreased. In a certain concentration range, the content of MDA decreased, there was a dose-effect relationship between the groups, the concentration exceeded this range, the effect was weakened, and there were significant differences among different dose groups. Acute death model was established. Drosophila melanogaster fed with different doses of Hawthorn alcohol extract was treated with Paraquat hydrogen peroxide to induce hydroxyl radical and superoxide anion oxidation. The life span of fruit fly in high dose group was higher than that in low dose group. The above experiments proved that Hawthorn extract has strong antioxidation in vivo and can be used to develop anti-aging health food.
【學(xué)位授予單位】：天津科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R285.5;R-332

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