本文選題：脂肪肝 + 含patatin樣磷脂酶域��； 參考：《臨床肝膽病雜志》2016年09期

【摘要】：目的觀察含patatin樣磷脂酶域3(PNPLA3)和膽固醇調(diào)節(jié)元件結(jié)合蛋白(SREBP)1c在藍(lán)莓聯(lián)合益生菌對非酒精性脂肪性肝病(NAFLD)模型大鼠干預(yù)實(shí)驗(yàn)中的表達(dá),并探討其在NAFLD中的作用機(jī)制。方法清潔級(jí)SD大鼠36只分為正常組(NG)、模型組(MG)、自然恢復(fù)組(SRG)、藍(lán)莓組(BG)、益生菌組(PG)和藍(lán)莓聯(lián)合益生菌組(BPG)。實(shí)驗(yàn)結(jié)束后,制作組織切片,觀察血清學(xué)、組織學(xué)形態(tài)變化,檢測PNPLA3和SREBP-1c基因及蛋白表達(dá)情況。多組間比較用單因素方差分析,方差齊時(shí)用SNK-q檢驗(yàn),方差不齊時(shí)用Tamhane's-T2檢驗(yàn)。結(jié)果 6組間在肝臟指數(shù)、ALT、TC、TG、LDL和HDL上差異均有統(tǒng)計(jì)學(xué)意義(F值分別為384.908、188.554、75.523、94.667、95.235、132.586,P值均0.01)。6組間NAFLD活動(dòng)度積分差異有統(tǒng)計(jì)學(xué)意義(F=71.896,P0.01),其中BPG組較MG、SRG、BG、PG組下降最為顯著(P值均0.01)。6組肝組織表達(dá)PNPLA3和SREBP-1c陽性率差異均有統(tǒng)計(jì)學(xué)意義(F值分別為175.527、110.504,P值均0.01),其中BPG組較MG、SRG、BG、PG組下降最為顯著(P值均0.01)。PNPLA3和SREBP-1c的mRNA和蛋白表達(dá)各組間差異有統(tǒng)計(jì)學(xué)意義(F值分別為375.822、410.379、288.940、116.054,P值均0.01)。其中BPG組的PNPLA3和SREBP-1C的mRNA和蛋白表達(dá)較MG、SRG、BG、PG組明顯下降(P值均0.01)。結(jié)論藍(lán)莓聯(lián)合益生菌能有效改善NAFLD的病理組織結(jié)構(gòu),減輕肝細(xì)胞脂肪變性,其機(jī)制可能是藍(lán)莓與益生菌聯(lián)合可減輕炎癥因子誘發(fā)的炎癥效應(yīng),下調(diào)SREBP-1c的表達(dá),從而減弱PNPLA3基因轉(zhuǎn)錄,增強(qiáng)膽固醇代謝,減少脂質(zhì)沉積,是NAFLD的一個(gè)輔助治療方案。
[Abstract]:Objective to observe the expression of patatin-like phospholipase domain 3 (PNPLA3) and cholesterol regulatory element binding protein (SREBP1c) in the intervention experiment of blueberry combined with probiotics in rats with nonalcoholic fatty liver disease (NAF LDD) and to explore the mechanism of its role in NAFLD. Methods Thirty-six SD rats of clean grade were divided into normal group, model group, natural recovery group, blueberry group, probiotics group (PGN) and blueberry combined probiotics group. At the end of the experiment, tissue sections were made, serological and histological changes were observed, and the expression of PNPLA3 and SREBP-1c genes and proteins were detected. Single factor analysis of variance (ANOVA) was used in multigroup comparison, SNK-q test was used when variance was homogeneous, and Tamhane's-T2 test was used when variance was uneven. Results there were significant differences in NAFLD activity scores between the six groups on the liver index (alt) and HDL (P = 384.908188.554, P = 75.5239.667.65.235132.586p, respectively). There was a significant difference in NAFLD activity between the six groups (F71.896a, P0.01P). The BPG group had the most significant decrease compared with the MGG group (P < 0.01). The difference of PNPLA3 and SREBP-1c positive rate was statistically significant (F = 175.527110.504 P = 0.01), in which the mRNA and protein expression of 0.01).PNPLA3 and SREBP-1c in BPG group were significantly lower than those in MGG BPG group (P = 375.822410.379, 288.940116.054, P < 0.01). The expression of mRNA and protein in PNPLA3 and SREBP-1C in BPG group was significantly lower than that in MGG group. Conclusion the combination of blueberry and probiotics can effectively improve the pathological structure of NAFLD and reduce the steatosis of hepatocytes. The mechanism may be that the combination of blueberry and probiotics can reduce the inflammatory effect induced by inflammatory factors and down-regulate the expression of SREBP-1c. It is an adjuvant therapy for NAFLD to reduce the transcription of PNPLA3 gene, enhance cholesterol metabolism and reduce lipid deposition.
【作者單位】： 貴州醫(yī)科大學(xué)附屬醫(yī)院感染科;
【基金】：貴州省科技廳社會(huì)攻關(guān)資助項(xiàng)目(黔科合SY[2010]3017號(hào)) 黔東南苗族侗族自治州林業(yè)局科研基金資助項(xiàng)目(ZLYJZFCG-2012-7)
【分類號(hào)】：R575.5;R-332

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