本文選題：多能干細胞 + 腫瘤細胞HeLa　； 參考：《信陽師范學院學報(自然科學版)》2017年01期

【摘要】：將攜帶4種轉(zhuǎn)錄因子的逆轉(zhuǎn)錄病毒共同感染HeLa細胞,感染后的細胞鋪到滋養(yǎng)層細胞MEF上培養(yǎng),細胞長出類似胚胎干細胞的克隆團,這種克隆團可以擴增,并進一步誘導分化為神經(jīng)元,同時免疫熒光檢測細胞分化不同天數(shù)后Pax6,Sox1和βⅢ-tubulin的表達.結(jié)果表明,腫瘤細胞HeLa在Oct4、Sox2、c-Myc、Klf4等4種轉(zhuǎn)錄因子的作用下成功重編程為多能干細胞,同時可進一步分化為神經(jīng)元.
[Abstract]:Retroviruses carrying four transcription factors were co-infected with HeLa cells. The infected cells were cultured on the MEF of trophoblast cells, and the cells grew clones similar to embryonic stem cells, which could be expanded. The expression of Pax6, Sox1 and 尾 鈪，

本文編號：1870499