發(fā)布時(shí)間：2018-05-10 19:03

  本文選題：多能干細(xì)胞 + 腫瘤細(xì)胞HeLa��； 參考：《信陽(yáng)師范學(xué)院學(xué)報(bào)(自然科學(xué)版)》2017年01期

【摘要】：將攜帶4種轉(zhuǎn)錄因子的逆轉(zhuǎn)錄病毒共同感染HeLa細(xì)胞,感染后的細(xì)胞鋪到滋養(yǎng)層細(xì)胞MEF上培養(yǎng),細(xì)胞長(zhǎng)出類似胚胎干細(xì)胞的克隆團(tuán),這種克隆團(tuán)可以擴(kuò)增,并進(jìn)一步誘導(dǎo)分化為神經(jīng)元,同時(shí)免疫熒光檢測(cè)細(xì)胞分化不同天數(shù)后Pax6,Sox1和βⅢ-tubulin的表達(dá).結(jié)果表明,腫瘤細(xì)胞HeLa在Oct4、Sox2、c-Myc、Klf4等4種轉(zhuǎn)錄因子的作用下成功重編程為多能干細(xì)胞,同時(shí)可進(jìn)一步分化為神經(jīng)元.
[Abstract]:Retroviruses carrying four transcription factors were co-infected with HeLa cells. The infected cells were cultured on the MEF of trophoblast cells, and the cells grew clones similar to embryonic stem cells, which could be expanded. The expression of Pax6, Sox1 and 尾 鈪，

本文編號(hào)：1870499