本文選題：臍帶 + 間充質(zhì)干細(xì)胞　； 參考：《中國藥理學(xué)通報(bào)》2017年01期

【摘要】：目的探討生理和炎性微環(huán)境下人臍帶間充質(zhì)干細(xì)胞(human umbilical cord derived mesenchymal stem cells,h UCMSCs)能否通過外泌體誘導(dǎo)調(diào)節(jié)性T細(xì)胞的生成來發(fā)揮免疫抑制功能。方法膠原酶消化法分離h UC-MSCs,應(yīng)用流式細(xì)胞術(shù)鑒定h UC-MSCs。IFN-γ模擬體內(nèi)炎性微環(huán)境,以未預(yù)處理為對(duì)照,分別提取外泌體,得到Nor-h UC-exo和IFN-γ-stimulated h UC-exo,分析其濃度及粒徑分布等特征、并鑒定表面標(biāo)記蛋白CD63的表達(dá)。采用h UC-MSCs、Nor-h UCexo、IFN-γpretreated-h UC-MSCs、IFN-γstimulated h UC-exo與人外周血單個(gè)核細(xì)胞共培養(yǎng)5 d后,流式細(xì)胞術(shù)檢測T細(xì)胞的增殖、調(diào)節(jié)性T細(xì)胞的比例變化。結(jié)果 h UC-MSCs高表達(dá)CD73、CD44等間充質(zhì)干細(xì)胞標(biāo)志物。IFN-γ刺激前后外泌體粒徑無明顯變化,但I(xiàn)FN-γ刺激后分泌量和CD63增加(P0.05)。CFSE染料示蹤結(jié)果顯示,h UC-MSCs來源的外泌體抑制PBMCs增殖(P0.01),且IFN-γ刺激后明顯提高外泌體的免疫抑制能力(P0.01)。在活化T細(xì)胞中,IFN-γ-stimulated h UC-exo組Treg比例(11.53±0.88%)與IFN-γpretreated-h UC-MSCs組(7.54±0.50%)(P0.05)、Norh UC-exo組(6.60±0.56%)(P0.01)、對(duì)照組(3.87±0.73%)(P0.01)相比升高。結(jié)論 h UC-MSCs可通過分泌外泌體來發(fā)揮免疫調(diào)節(jié)作用,在炎癥因子刺激下h UCMSCs分泌的外泌體明顯促進(jìn)調(diào)節(jié)性T細(xì)胞的生成,h UC-exo可能是潛在的免疫抑制載體。
[Abstract]:Objective to investigate whether human umbilical cord derived mesenchymal stem cells can induce regulatory T cell formation in human umbilical cord mesenchymal stem cells (UCMSCs) in physiological and inflammatory microenvironment to play an immunosuppressive role. Methods the hUC-MSCswere isolated by collagenase digestion. The inflammatory microenvironment of hUC-MSCs.IFN- 緯 was identified by flow cytometry. The exocrine was extracted from the unpretreated control, and the Nor-h UC-exo and IFN- 緯 -stimulated UC-exo were obtained, and their concentration and particle size distribution were analyzed. The expression of surface marker protein CD63 was identified. Human peripheral blood mononuclear cells (PBMC) were co-cultured for 5 days with hUC-MSCsCoculture of IFN- 緯 pretreated-h stimulated h UC-exo. The proliferation of T cells and the percentage of regulatory T cells were detected by flow cytometry. Results h UC-MSCs overexpression of CD73, CD44 and other mesenchymal stem cell markers, such as. IFN- 緯, had no significant change in the size of exocrine. But after IFN- 緯 stimulation, the secretion and CD63 increased P0.05N 路CFSE dye tracer. The results showed that exosomes derived from UC-MSCs inhibited the proliferation of PBMCs P0.01, and IFN- 緯 increased the immunosuppressive ability of exocrine. The ratio of Treg in IFN- 緯 -stimulated h UC-exo group (11.53 鹵0.88) was significantly higher than that in IFN- 緯 pretreated-h UC-MSCs group (7.54 鹵0.50) and the control group (3.87 鹵0.73). Conclusion h UC-MSCs can play an immunomodulatory role by secreting exosomes, and the secretion of h UCMSCs stimulated by inflammatory cytokines can obviously promote the formation of regulatory T cells. H UC-exo may be a potential immunosuppressive vector.
【作者單位】： 南方醫(yī)科大學(xué)藥學(xué)院;廣東省人民醫(yī)院醫(yī)學(xué)研究中心廣東省醫(yī)學(xué)科學(xué)院;廣東食品藥品職業(yè)學(xué)院國際交流學(xué)院;廣州醫(yī)科大學(xué)藥學(xué)院;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No 81330007,81120108003) 廣東省科技計(jì)劃重點(diǎn)項(xiàng)目(No2015B020225006);廣東省科技計(jì)劃國際合作項(xiàng)目(No2014A05053047)
【分類號(hào)】：R392

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