本文選題：人乳頭瘤病毒 + E蛋白��； 參考：《中國(guó)病原生物學(xué)雜志》2017年08期

【摘要】：目的應(yīng)用生物信息學(xué)預(yù)測(cè)分析人乳頭瘤16型病毒(human papillomavirus type 16,HPV16)E7蛋白的結(jié)構(gòu)特征與抗原表位。方法自NCBI GENE BANK獲取HPV16E7蛋白的基因信息,應(yīng)用ProtParam分析其編碼的E7蛋白理化性質(zhì)和一級(jí)結(jié)構(gòu);利用SOPMA分析E7蛋白的二級(jí)結(jié)構(gòu);應(yīng)用SWISS MODEL的Automated mode預(yù)測(cè)E7蛋白的三維空間結(jié)構(gòu);使用NetNGlyc 1.0Server、NetPhos3.1Server、MotifyScan、SignalP及TMHMM預(yù)測(cè)蛋白質(zhì)的翻譯后修飾位點(diǎn)、信號(hào)肽切割位點(diǎn)和跨膜區(qū);利用CLUSTALX將HPV16E7蛋白同其他高危型HPV E7蛋白進(jìn)行同源性對(duì)比;應(yīng)用ABCpred、BepiPred、IEDB、SYFPEITHI和NetMHCIIpan 3.1Server預(yù)測(cè)E7蛋白的優(yōu)勢(shì)抗原表位。結(jié)果生物信息學(xué)預(yù)測(cè)E7蛋白無(wú)規(guī)卷曲占52.04%,結(jié)構(gòu)較松散,為不穩(wěn)定性蛋白。該蛋白存在多個(gè)磷酸化位點(diǎn)。利用序列匹配度為46%的HPV45E7蛋白為模板得到同型二聚體三級(jí)預(yù)測(cè)結(jié)構(gòu)。E7蛋白高度保守,且具有潛在的B細(xì)胞抗原表位1-8、15-22、33-46,CD8+T細(xì)胞抗原表位82-90、11-19、7-11、85-93、13-21和CD4+T細(xì)胞抗原表位9-23、73-87、10-24、7-21、21-35。結(jié)論 HPV16E7蛋白為不穩(wěn)定蛋白。該蛋白高度保守,且具有潛在的B、T細(xì)胞抗原表位。
[Abstract]:Objective to predict and analyze the structure and epitope of HPV16 E7 protein of human papillomavirus type 16 papillomavirus type 16 by bioinformatics. Methods the gene information of HPV16E7 protein was obtained from NCBI GENE BANK, the physicochemical properties and primary structure of E7 protein were analyzed by ProtParam, the secondary structure of E7 protein was analyzed by SOPMA, the three-dimensional structure of E7 protein was predicted by Automated mode of SWISS MODEL. Using NetNGlyc 1.0 Server, NetPhos3.1 Server motif y ScanSignalP and TMHMM to predict post-translational modification sites, signal peptide cleavage sites and transmembrane regions, CLUSTALX was used to compare HPV16E7 protein homology with other high-risk HPV E7 proteins, and ABCpredNetNGlyc BepiPredIEDBSYFPEITHI and NetMHCIIpan 3.1Server were used to predict the dominant epitope of E7 protein. Results the random coil of E7 protein in bioinformatics was 52.04% and its structure was looser and it was an unstable protein. There are many phosphorylation sites in this protein. Using 46% HPV45E7 protein as template, the homotypic dimer tertiary predictive structure. E7 protein was highly conserved, and had potential B cell epitope 1-82233-46-CD8 T cell epitope 82-90 11-197-1175-95-9313-21 and CD4 T cell epitope 9-2373-83-83-81-2121-3535. Conclusion HPV16E7 protein is unstable protein. The protein is highly conserved and has a potential epitope of BU T cell antigen.
【作者單位】： 中國(guó)醫(yī)科大學(xué)臨床三系;中國(guó)醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院病原生物學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(No.81472439,81101989)
【分類(lèi)號(hào)】：R373

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