本文選題：氫氧化鋁 + 黃連堿�。� 參考：《中國公共衛(wèi)生》2017年05期

【摘要】：目的探討氫氧化鋁與黃連堿復(fù)合佐劑對甲肝疫苗誘導(dǎo)小鼠體液免疫的影響。方法選取6~8周齡雌性ICR小鼠49只,隨機分為空白組、單純抗原組、單純黃連堿佐劑組、鋁佐劑組及復(fù)合佐劑低、中、高劑量組,每組7只;在免疫后第4、8、12、16周,采用間接酶聯(lián)免疫吸附測定法(ELISA)檢測小鼠分離血清中的抗HAV IgG抗體水平。結(jié)果在免疫后第4、8、12、16周,除空白組外,各組小鼠均能檢測到抗HAV IgG抗體,且抗體水平隨免疫后時間的延長均先升高而后降低;在免疫后第12周,各組小鼠血清中抗HAV IgG抗體水平達到峰值,單純抗原組、單純黃連堿佐劑組、鋁佐劑組及復(fù)合佐劑低、中、高劑量組小鼠分別為log_(10)(2.908±0.413)、log_(10)(2.960±0.315)、log_(10)(3.460±0.295)、log_(10)(3.460±0.351)、log_(10)(3.470±0.251)、log_(10)(3.470±0.251);在免疫后第4周,復(fù)合佐劑低劑量組小鼠抗HAV IgG抗體水平均高于單純抗原組和單純黃連堿佐劑組(均P0.05);在免疫后第8、12、16周,復(fù)合佐劑低、中、高劑量組小鼠抗HAV IgG抗體水平均高于單純抗原組和單純黃連堿佐劑組(均P0.05);在免疫后第12周,鋁佐劑組小鼠抗HAV IgG抗體水平均高于單純抗原組和單純黃連堿佐劑組(均P0.05);在免疫后第16周,鋁佐劑組小鼠抗HAV IgG抗體水平高于單純抗原組(P0.05)。結(jié)論適當(dāng)配比的黃連堿和氫氧化鋁復(fù)合佐劑可快速、顯著提高甲肝疫苗誘導(dǎo)小鼠的體液免疫應(yīng)答,有望成為一種新的人用疫苗佐劑。
[Abstract]:Objective to investigate the effect of compound adjuvant of aluminum hydroxide and Coptidine on humoral immunity of mice induced by hepatitis A vaccine. Methods Forty-nine female ICR mice aged 6 to 8 weeks were randomly divided into blank group, simple antigen group, simple salicylide adjuvant group, aluminum adjuvant group and compound adjuvant group with 7 rats in each group. Indirect enzyme linked immunosorbent assay (Elisa) was used to detect the level of anti HAV IgG antibody in isolated serum of mice. Results Antibody to HAV IgG was detected in all mice except the blank group at the 4th week and 12th week after immunization, and the antibody level increased first and then decreased with the prolongation of the time after immunization, and then decreased at the 12th week after immunization. The levels of anti HAV IgG antibody in serum of mice in each group reached a peak. The levels of anti HAV IgG antibody in the serum of the mice in the antigen alone group, the simple Coptis alkaloid adjuvant group, the aluminum adjuvant group and the compound adjuvant group were low. In the middle and high dose groups, the levels of log_(10)(2.908 鹵0. 413 + HAV IgG + 10 0 960 鹵0. 315 + 0. 315% P / D were 3. 460 鹵0. 295 and 3. 460 鹵0. 351 respectively, and in the fourth week after immunization, they were 3. 470 鹵0. 251 鹵0. 251P and 3. 470 鹵0. 251P, respectively, and in the fourth week after immunization, the levels of anti HAV IgG antibody in the mice were 3. 470 鹵0. 251 鹵0. 251 鹵0. 251P, respectively. The levels of anti HAV IgG antibody in the low dose group of compound adjuvant were higher than those in the group of simple antigen and the group of only Coptis alkalide adjuvant (P0.05), and the level of the complex adjuvant was low and moderate at 812 ~ 16 weeks after immunization. The levels of anti HAV IgG antibody in the high dose group were higher than those in the simple antigen group and the Coptis alkaloid adjuvant group (all P 0 05). The level of anti HAV IgG antibody in the aluminum adjuvant group was higher than that in the simple antigen group and the Coptis alkalide adjuvant group (all P 0.05), and the level of anti HAV IgG antibody in the aluminum adjuvant group was higher than that in the simple antigen group at the 16th week after immunization. Conclusion the compound adjuvant of Coptis chinensis and aluminum hydroxide can improve the humoral immune response of mice induced by hepatitis A vaccine, and it is expected to be a new adjuvant for human vaccine.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院醫(yī)學(xué)生物學(xué)研究所;云南省蟲媒傳染病防控研究重點實驗室;昆明醫(yī)科大學(xué);
【基金】：國家科技支撐計劃項目(2014BAI01B01) 國家自然科學(xué)基金(31500748)
【分類號】：R392
，

本文編號：1863475