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  本文選題：睡眠剝奪 + 對氯苯丙氨酸��； 參考：《福建醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的: 1.研究快速眼動睡眠剝奪(RSD)和對氯苯丙氨酸(PCPA)所致完全性失眠兩種睡眠剝奪(SD)模型對大鼠學(xué)習(xí)記憶及血漿中單胺類神經(jīng)遞質(zhì)去甲腎上腺素(NE)、多巴胺(DA)及5-羥色胺(5-HT)的含量的影響。 2.研究松郁安神方和阿普唑侖對兩種SD模型干預(yù)后大鼠學(xué)習(xí)記憶及血漿中單胺類神經(jīng)遞質(zhì)含量的變化。 方法: 健康雄性Sprague-Dawley大鼠88只,體重200±10g,隨機(jī)選取其中40只用于建立SD模型。采用改良多平臺法(MMPM)建立RSD模型,采用腹腔注射PCPA建立完全性失眠模型,分成5組:空白對照(CC)組、大平臺環(huán)境對照(TC)組、快速眼動睡眠剝奪(RSD)組、腹腔注射PCPA組、腹腔注射生理鹽水(NS)對照組。另外48只用于藥物干預(yù):建立兩種SD模型后分別用松郁安神方和阿普唑侖灌胃5天。大鼠分成6組:NS+蒸餾水組、PCPA+蒸餾水組、RSD+中藥組、RSD+西藥組、PCPA+中藥組、PCPA+西藥組。各組動物實驗前后行Morris水迷宮測試大鼠的學(xué)習(xí)記憶能力。實驗結(jié)束后,腹主動脈取血,用高效液相色譜-電化學(xué)檢測法(HPLC-ECD)檢測血漿中NE、DA、5-HT的含量。 結(jié)果: 1.大鼠學(xué)習(xí)記憶能力變化: 造模后Morris水迷宮測試各組的潛伏期較造模前均下降(P0.05)。RSD造模后,RSD組的潛伏期比TC組、CC組明顯延長(P0.05)。藥物干預(yù)后,與RSD組相比,RSD+中藥組和RSD+西藥組大鼠潛伏期縮短(P0.05)。 2.血漿中NE、DA、5-HT含量的變化: (1)SD模型組:與CC組、TC組、NS組相比,RSD組、PCPA組NE含量升高(P0.05)。與CC組、PCPA組相比,RSD組DA含量下降,與NS組相比,PCPA組DA含量升高(P0.05)。與CC組、NS組比較,RSD、PCPA組5-HT含量下降(P0.05)。 (2)藥物干預(yù)組:與RSD組、PCPA組、RSD+中藥組相比,PCPA+中藥組、PCPA+西藥組NE含量下降;與RSD組比較,RSD+西藥組NE含量下降;與PCPA組、PCPA+蒸餾水組比較,PCPA+中藥組DA下降;與PCPA組、RSD組比較,RSD+西藥組、PCPA+西藥組、PCPA+中藥組5-HT含量升高。 結(jié)論: 1.RSD使大鼠的學(xué)習(xí)記憶能力受損。PCPA對學(xué)習(xí)記憶能力無顯著影響。中藥及西藥灌胃均可使RSD大鼠學(xué)習(xí)記憶得到改善,提示藥物干預(yù)能提高機(jī)體的學(xué)習(xí)記憶能力。 2.RSD使血漿中NE含量升高,DA、5-HT含量下降,PCPA失眠使血漿中NE、DA含量上升,5-HT含量下降。 3.中藥干預(yù)后RSD大鼠血漿中5-HT含量升高,PCPA失眠大鼠NE、DA含量下降,5-HT含量升高。西藥干預(yù)后RSD大鼠NE含量下降、5-HT含量上升, PCPA失眠大鼠5-HT含量上升。神經(jīng)遞質(zhì)含量的變化提示藥物干預(yù)可能通過改變失眠者體內(nèi)的神經(jīng)遞質(zhì)調(diào)節(jié)睡眠。
[Abstract]:Objective: 1. The effects of rapid eye movement sleep deprivation (RSDs) and complete insomnia induced by p-chlorphenylalanine (PCPA) on learning and memory and the contents of monoamine neurotransmitter norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in plasma of rats were studied. 2. To study the changes of learning and memory and the content of monoamine neurotransmitters in plasma after the intervention of Songyu Anshen recipe and alprazolam on two SD models in rats. Methods: 88 healthy male Sprague-Dawley rats, weighing 200 鹵10 g, were randomly selected to establish SD model. The RSD model was established by modified multi-platform method and the complete insomnia model was established by intraperitoneal injection of PCPA. The model was divided into five groups: blank control group, large platform environment control group, rapid eye movement sleep deprivation group, and intraperitoneal injection of PCPA group. The control group was treated by intraperitoneal injection of NS. The other 48 rats were used for drug intervention: two SD models were established and treated with Songyu Anshen recipe and alprazolam respectively for 5 days. Rats were divided into 6 groups: PCPA distilled water group and PCPA distilled water group. The learning and memory abilities of rats were tested by Morris water maze before and after the experiment. After the experiment, the blood was taken from the abdominal aorta and the plasma NEDAD 5-HT was detected by high performance liquid chromatography-electrochemical detection (HPLC-ECD). Results: 1. Changes of learning and memory ability in rats: The latency of Morris water labyrinth test group was significantly longer than that of TC group. After drug intervention, compared with RSD group, the latency of rats in RSD group and RSD group was shortened (P 0.05). 2. Changes of 5-HT in plasma: The content of NE in PCPA group was higher than that in TC group (CC group) and NS group (P 0.05). Compared with CC group, the DA content of PCPA group was lower than that of PCPA group, and the DA content of PCPA group was higher than that of NS group. Compared with CC group and NS group, the 5-HT content of RSDN PCPA group was decreased (P 0.05). (2) Drug intervention group: compared with RSD group, the content of NE in PCPA group was lower than that in RSD group, the NE content in western medicine group was lower than that in RSD group, and the DA level in PCPA group was lower than that in PCPA group. Compared with the PCPA group, the content of 5-HT in the PCPA group was higher than that in the western medicine group. Conclusion: 1.RSD had no significant effect on learning and memory ability. Both Chinese medicine and western medicine could improve the learning and memory of RSD rats, suggesting that drug intervention could improve the ability of learning and memory. The content of NE in plasma was increased by 2.RSD and the content of 5-HT in plasma was decreased. The content of NEDA in plasma was increased and the content of 5-HT in plasma was decreased. 3. The content of 5-HT in plasma of RSD rats was increased after the intervention of traditional Chinese medicine. The content of NEDA in rats with RSD insomnia decreased and the content of 5-HT increased. After intervention of western medicine, NE content decreased and 5 HT content increased in RSD rats, and 5-HT content increased in PCPA insomnia rats. Changes in neurotransmitters suggest that drug intervention may regulate sleep by altering neurotransmitters in insomnia patients.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周運峰;王玉霞;;三部推拿法治療心脾兩虛型不寐的臨床及機(jī)理研究[J];中華中醫(yī)藥雜志;2006年05期

2 徐碧云;李艷;肖芳;歐碧陽;楊志敏;;加味逍遙散對心理應(yīng)激性失眠患者單胺類神經(jīng)遞質(zhì)的影響[J];中華中醫(yī)藥雜志;2009年06期

3 黃建歐,趙忠新;大鼠睡眠剝奪方法的研究進(jìn)展[J];中華神經(jīng)醫(yī)學(xué)雜志;2004年03期

4 葉晨靜;趙忠新;;快速眼球轉(zhuǎn)動期睡眠剝奪對大鼠學(xué)習(xí)、記憶能力及其海馬組織腦源性神經(jīng)營養(yǎng)因子表達(dá)的影響[J];第二軍醫(yī)大學(xué)學(xué)報;2007年01期

5 楊國愉,馮正直,皇甫恩,苗丹民,王家同;人參皂甙對睡眠剝奪下大鼠腦干中縫核群5-HT的影響[J];第三軍醫(yī)大學(xué)學(xué)報;2002年02期

6 江帆,顏崇淮,沈曉明;與睡眠相關(guān)的中樞單胺類遞質(zhì)[J];國外醫(yī)學(xué).精神病學(xué)分冊;2001年01期

7 曹信杰;;針刺地機(jī)穴治療頑固性不寐38例[J];河北中醫(yī);2006年06期

8 孫存濟(jì);張雋彝;;對氯苯丙氨酸及其酯的制備[J];化學(xué)試劑;1981年05期

9 宋媛;趙倉煥;任莉;;不同穴位處方麥粒灸對失眠大鼠下丘腦單胺類神經(jīng)遞質(zhì)的影響[J];暨南大學(xué)學(xué)報(自然科學(xué)與醫(yī)學(xué)版);2009年02期

10 鄭璐;邱蕾;張瑤;馮天驕;于建春;張占軍;;遠(yuǎn)志皂苷對快速腦老化鼠學(xué)習(xí)記憶能力的改善及對神經(jīng)遞質(zhì)的影響[J];北京中醫(yī)藥大學(xué)學(xué)報;2010年03期

相關(guān)碩士學(xué)位論文 前1條

1 劉彤;睡眠剝奪對大鼠學(xué)習(xí)能力的影響及機(jī)制實驗研究[D];天津醫(yī)科大學(xué);2007年

，

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