本文選題：前列腺素E_1 + 巨噬細胞　； 參考：《河南師范大學》2011年碩士論文

【摘要】：目的:通過觀察前列腺素E_1對正常小鼠子宮巨噬細胞表型和功能活性的影響,探討其免疫抑制機理以及與母胎免疫耐受之間的關(guān)系。方法:正常昆明種小鼠隨機分為正常對照組(DZ)和三個實驗組:低濃度組(D組),中濃度組(Z組),高濃度組(G組),實驗組分別尾靜脈注射1303ng/kg/d、2606ng/kg/d、3909ng/kg/d的前列腺素E_1(將前列腺素E_1溶于0.2ml生理鹽水),相應對照組分別用滅菌生理鹽水(0.2ml)處理,連續(xù)注射5d,分別于末次注射后1h、3h、6h、12h、36h取子宮,制冰凍切片與石蠟切片,并用非特異性酯酶染色與免疫組織化學方法檢測子宮巨噬細胞活性及其功能活性的變化。結(jié)果:(1)與對照組相比,各個實驗組注射前列腺素1h后子宮內(nèi)膜、子宮肌層和子宮外膜CD14~+巨噬細胞明顯減少(P0.01),CD14~+表達下降。注射PGE_13h后,各濃度實驗組子宮內(nèi)膜、子宮外膜巨噬細胞均極顯著減少,CD14~+表達量降低,子宮肌層CD14~+巨噬細胞數(shù)極顯著性增加(P0.01),CD14~+表達量增加。注射PGE_16h后,各濃度實驗組子宮內(nèi)膜巨噬細胞均極顯著減少(P0.01),CD14~+表達量下調(diào),各濃度實驗組子宮外膜巨噬細胞顯著減少(P0.05),CD14~+表達量下調(diào)(2)對照組小鼠子宮未檢測到CD204~+的表達,而相應實驗組只在中濃度組的12h與36h組與高濃度組的6h、12h與36h組檢測到CD204~+的表達,且表達趨勢與CD14~+相似。高濃度組較中、低濃度組在36h極顯著性增加(P0.01)。(3)與對照組相比,各個實驗組注射前列腺素1h后子宮內(nèi)膜α-NAE+巨噬細胞均顯著減少,子宮外膜α-NAE+巨噬細胞均顯著增加。注射PGE_112h后,各實驗組的子宮內(nèi)膜巨噬細胞均顯著增加,子宮肌層巨噬細胞則極顯著性減少。注射PGE_136h后,高濃度實驗組子宮內(nèi)膜、子宮肌層、子宮外膜巨噬細胞均極顯著增加。結(jié)論:(1)PGE_1可以抑制子宮巨噬細胞功能活性,促進其從子宮內(nèi)膜向子宮外膜遷移,這種效應與其半衰期與劑量呈相關(guān)性。(2)一定濃度的PGE_1能夠促進子宮巨噬細胞膜受體CD204~+的表達。(3)PGE_1通過調(diào)節(jié)子宮巨噬細胞的數(shù)量以及膜受體CD14~+、CD204~+的表達,從而參與妊娠期母胎免疫耐受的發(fā)生和維持。
[Abstract]:Aim: to investigate the effect of prostaglandin E _ 1 on the phenotype and functional activity of normal mouse uterine macrophages, and to explore the mechanism of its immunosuppression and the relationship between prostaglandin E _ 1 and maternal and fetal immune tolerance. Methods: normal Kunming mice were randomly divided into normal control group (DZ) and three experimental groups: low concentration group (D group), middle concentration group (group Z), high concentration group (group G) and caudal injection of prostaglandin E1 (prostaglandin E 1) of 1303 ng / kg / d 2606ng / kg / d respectively. The corresponding control group was treated with sterilized saline (0.2 ml), while the control group was treated with sterilized saline (0.2 ml). After 5 days of continuous injection, the uterus was collected at 1 h, 3 h, 6 h, 12 h and 36 h after the last injection. Frozen sections and paraffin sections were prepared. The changes of the activity and function of uterine macrophages were detected by non-specific esterase staining and immunohistochemical method. Results compared with the control group, the expression of CD14 ~ macrophage in myometrium and adventitia decreased significantly in each experimental group after 1 h of prostaglandin injection. After the injection of PGE_13h, the expression of CD14 ~ in the outer membrane macrophages was significantly decreased, and the number of CD14 ~ macrophages in the myometrium was significantly increased in each concentration group, and the expression of CD14 ~ was significantly increased in the myometrium of the uterus. After injection of PGE_16h, the expression of CD14 ~ in endometrial macrophages of each concentration group was significantly decreased, while that of adventitia macrophages in each concentration group was significantly decreased (2) the expression of CD204~ was not detected in the uterus of mice in the control group. The expression of CD204~ in the corresponding experimental group was detected only at 12h and 36h in the middle concentration group and 6h and 36h in the high concentration group, and the expression trend was similar to that in the CD14h group. Compared with the control group, the 偽 -NAE macrophages and 偽 -NAE macrophages in the endometrium of each experimental group were significantly decreased and the 偽 -NAE macrophages in the outer membrane of uterus were significantly increased after 1 h of prostaglandin injection. After PGE_112h injection, the endometrial macrophages in each experimental group increased significantly, while the myometrium macrophages decreased significantly. After PGE_136h injection, the number of endometrial, myometrium and adventitia macrophages in the experimental group increased significantly. Conclusion PGE1 can inhibit the functional activity of macrophages and promote the migration of macrophages from endometrium to the outer membrane of uterus. This effect was related to its half-life and dose. (2) PGE_1 at a certain concentration could promote the expression of CD204~ on the membrane receptor of uterine macrophage. PGE1 could regulate the number of macrophages and the expression of CD14 ~ + CD204~ in uterine macrophage. So as to participate in the development and maintenance of maternal and fetal immune tolerance during pregnancy.
【學位授予單位】：河南師范大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R363

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