本文選題：痛風(fēng)性關(guān)節(jié)炎 + 濕熱證�。� 參考：《湖南中醫(yī)藥大學(xué)》2012年碩士論文

【摘要】：目的：通過(guò)多因素復(fù)合造模的方法,以當(dāng)前廣泛應(yīng)用的濕熱證大鼠模型和經(jīng)典的痛風(fēng)性關(guān)節(jié)炎模型作為對(duì)照,探討建立中醫(yī)痛風(fēng)性關(guān)節(jié)炎濕熱證大鼠模型的方法,旨在開(kāi)展痛風(fēng)性關(guān)節(jié)炎濕熱證病證結(jié)合動(dòng)物模型研究,為病證結(jié)合治療的臨床療效機(jī)理研究提供新的思路和實(shí)驗(yàn)基礎(chǔ)。 方法：1、飲食高脂高糖飲食：普通飼料加用蜂蜜水自由飲用,且按大鼠體質(zhì)量,灌服油脂和白酒隔日交替。 2、分組選用健康成年SD大鼠40只,按隨機(jī)法將大鼠分為4組：空白對(duì)照組(A組),痛風(fēng)對(duì)照組(B組),濕熱對(duì)照組(C組),痛風(fēng)加濕熱模型組(D組),每組10只。 3、造模在高脂高糖飲食、濕熱環(huán)境的綜合因素作用下,建立濕熱證痛風(fēng)性關(guān)節(jié)炎動(dòng)物模型：①正常對(duì)照組：在恒溫、恒濕環(huán)境下,蒸餾水自由飲水,普通飼料喂養(yǎng)；②痛風(fēng)對(duì)照組：普通飼料+蒸餾水自由飲水+關(guān)節(jié)腔注射藥物；③濕熱對(duì)照組：高脂高糖飲食+濕熱環(huán)境；④痛風(fēng)加濕熱模型組：高脂高糖飲食+濕熱環(huán)境+關(guān)節(jié)腔注射藥物。 4、觀察及指標(biāo)檢測(cè)對(duì)比觀察實(shí)驗(yàn)大鼠造模前后的一般狀態(tài)、飲食量、飲水量、體重、大便性狀等的變化。用Bradford法檢測(cè)大鼠尿液中的水通道蛋白2(AQP2),放射免疫法檢測(cè)內(nèi)皮素(ET)及降鈣素基因相關(guān)肽(CGRP)。鼠處死后,迅速取出踝關(guān)節(jié)滑膜組織、舌組織,置于10%甲醛溶液中固定,石蠟包埋制成切片,HE染色,光鏡下觀察。 結(jié)果：內(nèi)皮素(ET)、降鈣素基因相關(guān)肽(CGRP)、AQP2作為濕熱證的量化指標(biāo),血尿酸、步態(tài)、關(guān)節(jié)組織形態(tài)學(xué)變化作為痛風(fēng)性關(guān)節(jié)炎的指標(biāo)。A組各項(xiàng)指標(biāo)為正常值(對(duì)照用),B組比較出現(xiàn)步態(tài)改變、關(guān)節(jié)滑膜組織形態(tài)學(xué)改變,C組出現(xiàn)內(nèi)皮素升高、降鈣素基因相關(guān)肽降低、尿AQP2含量降低,D組檢測(cè)結(jié)果與B、C兩組比較如果同時(shí)出現(xiàn)B、C兩組的改變(血尿酸,步態(tài)、關(guān)節(jié)組織形態(tài)學(xué)尿AQP2含量),提示該模型制作成功。 結(jié)論：實(shí)驗(yàn)結(jié)果顯示,在高脂高糖飲食+濕熱環(huán)境+關(guān)節(jié)腔注射藥物的綜合作用下,可以成功復(fù)制痛風(fēng)性關(guān)節(jié)炎濕熱證的動(dòng)物模型。此方法是對(duì)“病證結(jié)合”的痛風(fēng)性關(guān)節(jié)炎濕熱證動(dòng)物模型實(shí)驗(yàn)研究的積極探索,將對(duì)病證結(jié)合治療的臨床療效機(jī)理研究提供新的思路和實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:Objective: to establish a rat model of damp heat syndrome of gouty arthritis of traditional Chinese medicine by means of multi factor compound model making and the classic gouty arthritis model which is widely used as a contrast. The study of the mechanism of clinical efficacy provides new ideas and experimental basis.
Methods: 1. Diet high fat and high sugar diet: regular feed plus honey water for free consumption.
2, 40 healthy adult SD rats were selected, and the rats were divided into 4 groups according to random method: blank control group (group A), gout control group (group B), damp heat control group (group C), gout and damp heat model group (group D), 10 rats in each group.
3, under the action of high fat and high sugar diet and hot and humid environment, the model of damp heat syndrome of gouty arthritis was established. (1) normal control group: under constant temperature, constant humidity, free drinking water of distilled water, feeding of ordinary feed; 2. Gout control group: ordinary feed + steam distilled water free drinking water + joint injection medicine; damp heat pair Photo group: high fat and high sugar diet + damp heat environment; 4. Gout plus damp heat model group: high fat and high sugar diet + damp heat environment + joint cavity injection drugs.
4, observation and index detection were observed and compared to observe the general state of the rats before and after the experimental model, the amount of diet, the amount of drinking water, the weight and the stool character. The Bradford method was used to detect the aquaporin 2 (AQP2) in the urine of the rats, and the radioimmunoassay was used to detect the endothelin (ET) and calcitonin gene related peptide (CGRP). After the rat was killed, the synovial membrane group of the ankle joint was quickly removed. The tissues of tongue and tongue were fixed in 10% Formaldehyde Solution, and paraffin embedded into slices, stained with HE and observed under light microscope.
Results: endothelin (ET), calcitonin gene related peptide (CGRP), AQP2 as a quantitative index of damp heat syndrome, blood uric acid, gait and joint histomorphological changes as indexes of group.A for gouty arthritis were normal values (control). In group B, gait changes, histomorphological changes of synovial synovium in joint, and elevation of endothelin in group C were decreased. Calcitonin gene related peptide decreased, urine AQP2 content decreased, D group detection results compared with B, C two group if B, C two group changes (blood uric acid, gait, joint histomorphological AQP2 content), suggesting that the model was made successfully.
Conclusion: the experimental results show that the animal model of hygrothermal syndrome of gouty arthritis can be successfully replicated under the combined effect of high fat and high sugar diet + humid heat environment plus joint cavity injection. This method is an active exploration of the animal model of damp heat syndrome of gouty arthritis with the combination of disease and syndrome. The study of bed mechanism provides new ideas and experimental basis.

【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

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