本文選題：一般健康問(wèn)卷 + 血常規(guī)��； 參考：《大連醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的:本研究通過(guò)對(duì)不同人群一般健康狀況的調(diào)查,以及臨床實(shí)驗(yàn)指標(biāo)的檢測(cè),探討不同健康狀態(tài)對(duì)臨床實(shí)驗(yàn)指標(biāo)變化的影響,并且在分子生物學(xué)水平上研究不同健康狀態(tài)與HLA-DQB1等位基因的關(guān)聯(lián)。旨在了解不同健康狀態(tài)及個(gè)體差異對(duì)疾病的發(fā)生產(chǎn)生的影響。 方法: 1.選取職業(yè)人群32人(司機(jī)14人,護(hù)士18人),運(yùn)動(dòng)員36人分別作為觀察組,隨機(jī)選取正常健康體檢人員33人,作為對(duì)照組;高考人群26人,作為自身配對(duì)(備考期和恢復(fù)期),對(duì)以上人群統(tǒng)一發(fā)放一般健康問(wèn)卷,測(cè)量其一般健康狀況,并檢測(cè)其血常規(guī)、血清生化及免疫指標(biāo)。通過(guò)比較各組間量表的各項(xiàng)統(tǒng)計(jì)指標(biāo)及各項(xiàng)實(shí)驗(yàn)室檢測(cè)指標(biāo),探討不同健康狀態(tài)對(duì)臨床實(shí)驗(yàn)指標(biāo)變化的影響。 2.采用PCR技術(shù)檢測(cè)職業(yè)人群和對(duì)照組人群的HLA-DQB1等位基因,分析兩組之間HLA-DQB1等位基因分布頻率的差異,并研究其與臨床實(shí)驗(yàn)指標(biāo)之間的關(guān)系。 結(jié)果: 1.職業(yè)人群組和高考人群組量表的各項(xiàng)統(tǒng)計(jì)指標(biāo)差異均具有統(tǒng)計(jì)學(xué)意義,而運(yùn)動(dòng)員組未發(fā)現(xiàn)量表的各項(xiàng)指標(biāo)有統(tǒng)計(jì)學(xué)意義。 2.觀察組和對(duì)照組之間某些血常規(guī)、血清生化及免疫指標(biāo)的差異具有統(tǒng)計(jì)學(xué)意義(P0.05),相較于對(duì)照組,職業(yè)人群組嗜酸性粒細(xì)胞百分比(EO%)、血小板分布寬度(PDW)升高,血小板壓積(PCT)、平均血小板體積(MPV)、血小板(PLT)降低,葡萄糖(GLU)、肌酐(Cre)、白蛋白(ALB)、球蛋白(Glob)、總蛋白(TP)、總膽固醇(Chol)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、超敏c反應(yīng)蛋白(hs-CRP)升高,白球比(A/G)降低,免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、補(bǔ)體C3、補(bǔ)體C4升高;運(yùn)動(dòng)員組血小板(PLT)、血小板壓積(PCT)、淋巴細(xì)胞絕對(duì)值(LYMPH#)、嗜堿性粒細(xì)胞絕對(duì)值(BASO#)、嗜堿性粒細(xì)胞百分比(BASO%)、白蛋白(ALB)、葡萄糖(GLU)、肌酐(Cre)升高;高考前相較于高考后,葡萄糖(GLU)、高密度脂蛋白(HDL)升高,而肌酐(Cre)、低密度脂蛋白(LDL)、總蛋白(TP)、白蛋白(ALB)、球蛋白(Glob)降低。 3.職業(yè)人群組與對(duì)照組之間HLA-DQB1基因分布頻率差異不具有統(tǒng)計(jì)學(xué)意義(P0.05);HLA-DQB1*02位點(diǎn)對(duì)職業(yè)人群組紅細(xì)胞分布寬度系數(shù)(RDW-CV)的變化影響具有統(tǒng)計(jì)學(xué)意義,HLA-DQB1*06位點(diǎn)對(duì)職業(yè)人群組超敏c反應(yīng)蛋白(hs-CRP)的變化影響具有統(tǒng)計(jì)學(xué)意義(P0.05);HLA-DQB1*02位點(diǎn)對(duì)對(duì)照組白球比(A/G)、球蛋白(Glob)、免疫球蛋白A(IgA)的影響具有統(tǒng)計(jì)學(xué)意義,HLA-DQB1*04位點(diǎn)對(duì)對(duì)照組嗜酸性粒細(xì)胞絕對(duì)值(EO#)、嗜酸性粒細(xì)胞百分比(EO%)的影響具有統(tǒng)計(jì)學(xué)意義,HLA-DQB1*06位點(diǎn)對(duì)對(duì)照組總膽固醇(Chol)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)的影響具有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論:不同健康狀態(tài)對(duì)機(jī)體某些臨床實(shí)驗(yàn)指標(biāo)的變化有顯著影響,并且這種變化可能與HLA-DQB1等位基因表型有關(guān)。
[Abstract]:Objective: to investigate the influence of different health status on the changes of clinical experimental indexes by investigating the general health status of different population and the detection of clinical experimental indexes. The association between different health status and HLA-DQB1 allele was studied at molecular biological level. The purpose of this study was to understand the effects of different health states and individual differences on the occurrence of disease. Methods: 1. 32 professional people (14 drivers, 18 nurses, 36 athletes) were selected as observation group, 33 healthy persons as control group, 26 college entrance examination group, As self-matched (preparation period and convalescence period), the general health questionnaire was issued to the above population, their general health status was measured, and their blood routine, serum biochemical and immune indexes were tested. The effects of different health states on the changes of clinical experimental indexes were studied by comparing the statistical indexes and laboratory indicators of the scale. 2. PCR technique was used to detect the HLA-DQB1 alleles in the occupational population and the control group. The difference of the distribution frequency of HLA-DQB1 alleles between the two groups was analyzed and the relationship between HLA-DQB1 alleles and clinical trial indexes was studied. Results: 1. The differences between the professional group and the college entrance examination group scale were statistically significant, while the athletes group did not find any statistical significance. 2. Compared with the control group, the percentage of eosinophilic granulocytes in the occupational group was increased, and the platelet distribution width was increased, compared with the control group, the difference of serum biochemical and immunological indexes was statistically significant between the observation group and the control group (P < 0.05), and compared with the control group, the percentage of eosinophilic granulocytes in the occupational group was increased. The mean platelet volume (MPV), the platelet count (PLT) were decreased, the glucose gluconate (GLU), creatinine (creatine), Albumin (ALB), globulin (globulin), total protein (TTP), total cholesterol (TC) Choln, high density lipoprotein (HDL), low density lipoprotein (LDL), hypersensitive c reactive protein (hs-CRP) increased, and the white ball ratio was decreased. Immunoglobulin A (IgA), immunoglobulin G (IGG), complement C 3, complement C 4 were increased in athletes group, platelets in the athletes group were increased by PLTN, platelet compaction (PCT), lymphocyte absolute value (LYMP), basic granulocyte (basophil), basophilic granulocyte (basophil), percentage of basophilic granulocytes (basophil), Albumin (Alb), glucose gluconate (GLU), creatinine (creatinine). Compared with those before and after the college entrance examination, the levels of glucose gluconium and high density lipoprotein (HDL) increased, while creatinine (creatinine), low density lipoprotein (LDL), total protein (TPN), Albumin (Alb), globulin (globulin) decreased. 3. There was no significant difference in the frequency distribution of HLA-DQB1 gene between the occupational group and the control group. There was no significant difference in the distribution frequency of HLA-DQB1 gene between the occupational group and the control group. There was no significant difference in the frequency of HLA-DQB1 gene distribution between the occupational group and the control group. The effect of C-reactive protein hs-CRP) on HLA-DQB1O02 locus in the control group was statistically significant. There was statistical significance in the effect of HLA-DQB1O02 locus on the eosinophil absolute value, eosinophilic granulocyte, eosinophilic granulocyte in the control group (A / P / G / G), globulin and immunoglobulin A / IgA). The effect of HLA-DQB1 / 06 locus on the total cholesterol Cholla, HDL and LDL in the control group was statistically significant (P 0.05). Conclusion: different health status has a significant effect on the changes of some clinical experimental indexes, and this change may be related to the allelic phenotype of HLA-DQB1.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R346

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