發(fā)布時(shí)間：2018-05-01 03:21

  本文選題：三叉神經(jīng)痛 + CB1受體　； 參考：《南方醫(yī)科大學(xué)》2012年碩士論文

【摘要】：1、背景 大麻類植物是人類最早認(rèn)識(shí)的成癮性植物之一,使用大麻來(lái)止痛的記載可以追溯到數(shù)百年之前。最近十幾年,隨著內(nèi)源性大麻素(endogenous cannabinoids, EC)的發(fā)現(xiàn),以及兩種內(nèi)源性大麻素受體(cannabinoid type1receptor, CB1和cannabinoid type2receptor, CB2)的成功克隆,內(nèi)源性大麻素系統(tǒng)(endocannabinoid system,ECS)的存在已經(jīng)獲得初步證實(shí)。CBl、CB2受體都屬于G蛋白偶聯(lián)受體,CBl受體由473個(gè)氨基酸,7個(gè)跨膜結(jié)構(gòu)域構(gòu)成。研究發(fā)現(xiàn)CBl受體主要分布于神經(jīng)系統(tǒng),以腦組織中表達(dá)為主,集中分布在大腦的海馬、小腦和紋狀體,多定位于神經(jīng)末梢突觸前膜,發(fā)揮調(diào)節(jié)神經(jīng)遞質(zhì)釋放的作用。在腎上腺,,心臟,肺,前列腺,脾臟和扁桃體等外周組織中也有微量表達(dá)。CB2受體由360個(gè)氨基酸,7個(gè)跨膜結(jié)構(gòu)域構(gòu)成,與CBl受體相反,CB2受體在中樞神經(jīng)元中濃度很低,其主要在免疫系統(tǒng)中存在,包括脾臟、T細(xì)胞、扁桃體、B細(xì)胞及單核細(xì)胞中表達(dá),主要發(fā)揮免疫調(diào)節(jié)作用,并能夠抑制多種神經(jīng)遞質(zhì)和細(xì)胞因子的釋放。有研究表明CBl受體的拮抗劑可以增強(qiáng)對(duì)有害刺激的敏感性,且能夠增強(qiáng)疼痛性神經(jīng)元的活性。另外,CBl受體的激動(dòng)劑可以抑制疼痛神經(jīng)元的興奮性,并且能夠產(chǎn)生良好的鎮(zhèn)痛效應(yīng)。相關(guān)研究還表明CBl受體能夠減少傷害性疼痛,從而抑制自發(fā)性疼痛的相關(guān)行為。 原發(fā)性三義神經(jīng)痛(Trigeminal neuralgia, TN)是一種神經(jīng)慢性疾病,為三叉神經(jīng)的分支分布區(qū)域里反復(fù)發(fā)作的刀割樣陣發(fā)性的劇痛,其特點(diǎn)為三叉神經(jīng)在口腔頜而部的某一分支或者幾個(gè)支分支分布區(qū)域內(nèi)突發(fā)的短暫而劇烈疼痛,并可長(zhǎng)期的固定在某一分支,尤以第二、三支多見(jiàn),亦可幾支同時(shí)受累,持續(xù)疼痛時(shí)間為數(shù)秒鐘到數(shù)分鐘不等,而間歇期可以沒(méi)有任何癥狀,其發(fā)作為自發(fā)性或由面部、口腔內(nèi)輕微的觸覺(jué)刺激所誘發(fā),大多數(shù)是單側(cè)發(fā)病,其中又以中老年人好發(fā)。目前,TN的發(fā)病機(jī)制尚不明確,確切的動(dòng)物模型還難以建立。國(guó)內(nèi)外學(xué)者大多認(rèn)為TN的主要發(fā)病因素是周圍因素,其中的血管壓迫學(xué)說(shuō)得到大多數(shù)學(xué)者的親睞,所以,TN的動(dòng)物模型基本上是通過(guò)眶下神經(jīng)(infraorbital branch of the trigeminal nerve, ION)環(huán)扎術(shù)來(lái)模擬血管壓迫現(xiàn)象,并且在ION支配區(qū)域的表面皮膚進(jìn)行外在的機(jī)械性刺激,以此模擬TN扳機(jī)點(diǎn)來(lái)獲得。 三義神經(jīng)脊束核(Spinal trigeminal caudal subnucleus, Vc)從外到內(nèi)分為五層,在三叉神經(jīng)脊束的內(nèi)側(cè)和延髓的外側(cè)。Vc也是口腔頜面部組織的傷害性刺激向中樞傳導(dǎo)的第一道門戶,其尾側(cè)亞核及其臨近區(qū)域是軀體感覺(jué)的初級(jí)感受中樞,傳遞口腔頜面部的傷害性刺激信息。Vc的尾側(cè)亞核在細(xì)胞構(gòu)筑上相當(dāng)于脊髓后角Ⅰ～Ⅵ層。Vc也被認(rèn)為是面部傷害性傳導(dǎo)通路的二級(jí)神經(jīng)元所在區(qū)域,其與痛覺(jué)沖動(dòng)的傳遞和調(diào)制有著密切的關(guān)系。Vc的Ⅱ?qū)又心z狀質(zhì)中間神經(jīng)元發(fā)生變性,便喪失了對(duì)傳入的疼痛刺激的調(diào)節(jié)作用,失去了其對(duì)傳入沖動(dòng)的閘門作用,傳入沖動(dòng)可以很快達(dá)到一定的總和而引起疼痛劇烈發(fā)作。因此我們采用Vc組織來(lái)檢測(cè)CBl受體的表達(dá)變化。 有研究表明,損傷大鼠和猴子的外周神經(jīng),其存在于脊髓及背根神經(jīng)節(jié)神經(jīng)元中的μ阿片受體表達(dá)發(fā)生下降。而在坐骨神經(jīng)(CCI)后的大鼠模型的同側(cè)背角的表淺脊髓中CBl受體的表達(dá)同損傷大鼠單側(cè)神經(jīng)后的對(duì)側(cè)丘腦區(qū)域內(nèi)CBl受體的表達(dá)均發(fā)生上調(diào)。Nomura等和我們的研究團(tuán)隊(duì)的研究也都發(fā)現(xiàn),對(duì)橫斷單側(cè)下牙槽神經(jīng)的大鼠鼻口部進(jìn)行有害和無(wú)害的機(jī)械性刺激,則Fos和cdk5/p35蛋白樣免疫反應(yīng)性在其兩側(cè)Vc組織中均可被誘導(dǎo),并且橫斷的對(duì)側(cè)少于橫斷側(cè)。Vc作為感覺(jué)中繼站核,分別接受經(jīng)三義神經(jīng)傳入的頭而部感覺(jué)信息。坐骨神經(jīng)CCI之后CBl受體表達(dá)發(fā)生上調(diào)的機(jī)制仍不明了,但是在該模型中,脊柱CBl受體的表達(dá)可能受到蛋白激酶C、酪氨酸激酶受體和相關(guān)的細(xì)胞內(nèi)絲裂原活化蛋白激酶的調(diào)控。該模型的病理學(xué)特征與眶下神經(jīng)慢性壓迫性損傷(chronic constriction injury on the infraorbital branch of the trigeminal nerve, ION-CCI)大鼠模型的病理學(xué)特征相類似。 綜合上述的研究背景,本實(shí)驗(yàn)制備了三叉神經(jīng)痛大鼠模型并檢測(cè)了各組三叉神經(jīng)痛大鼠模型Vc組織中CBl受體的表達(dá)變化。 2、目的 我們假設(shè)TN大鼠模型的Vc組織中的神經(jīng)元內(nèi)存在CBl受體,因?yàn)槟壳叭詿o(wú)TN大鼠模型中CBl受體如何發(fā)揮相關(guān)作用方而的論述,此外,延髓下段的Vc組織中的神經(jīng)元接受三叉神經(jīng)的感覺(jué)神經(jīng)傳導(dǎo)的相關(guān)信息,所以,我們將TN的實(shí)驗(yàn)研究模型定為ION-CCI的大鼠模型,檢驗(yàn)ION-CCI后大鼠Vc組織中CBl受體的表達(dá)變化,目的是為研究CBl受體與TN的臨床治療及致病機(jī)理的關(guān)聯(lián)性打下實(shí)驗(yàn)基礎(chǔ)。 3、材料與方法 3.1實(shí)驗(yàn)動(dòng)物分組 同一批次健康成體雄性SD大鼠,體重為300-350g,36只隨機(jī)分為6組,每組6只。分為正常組、假手術(shù)組、ION-CCI1d組、ION-CCI3d組、ION-CCI7d組、ION-CCI14d組。雄性SD大鼠由南方醫(yī)科大學(xué)珠江醫(yī)院動(dòng)物實(shí)驗(yàn)室以常規(guī)固體飼料飼養(yǎng),溫度控制在25℃左右,濕度在45%-50%之間,12h明暗循環(huán)(07：00—19：00)。為了消除因?yàn)閯?dòng)物行為的隨意性對(duì)實(shí)驗(yàn)過(guò)程和結(jié)果的干擾,減少由此引起的假陽(yáng)性結(jié)果,在實(shí)驗(yàn)開始之前每口08：00開始,用一自制塑料棒敲擊籠壁四周和籠頂、觸摸、提拿大鼠,于大鼠習(xí)慣之后,待大鼠平靜時(shí),用一自制毛刷刺激大鼠須墊部,3次/每側(cè),每次刺激需連續(xù)2次,兩次刺激間隔不應(yīng)少于30秒。兩側(cè)交替進(jìn)行,直致大鼠從開始的抽鼻、探究、恐懼,甚至快速后退逃避、抓咬攻擊等轉(zhuǎn)為平靜。 3.2暴露并行眶下神經(jīng)結(jié)扎術(shù) 實(shí)驗(yàn)動(dòng)物腹腔內(nèi)注射10%水合氯醛(350mg/kg),將麻醉效果滿意的大鼠仰臥并固定其頭部和四肢,沿著其右側(cè)第一磨牙齦頰側(cè)邊緣,向其口鼻方向縱向切開長(zhǎng)約1cm的切口,分離暴露ION及周圍約5mm神經(jīng)組織,在肉眼或顯微鏡下使用兩根之間距離大約為2mmm的5.0鉻制腸線疏松環(huán)扎ION,結(jié)扎ION的緊張度要求是減小ION的直徑,使ION傳導(dǎo)延緩,不可以完全阻滯神經(jīng)表層血管的血液傳導(dǎo),血液循環(huán)必須通暢。術(shù)后6.0縫合線縫合傷口。假手術(shù)組大鼠除不結(jié)扎ION外,其他方面與手術(shù)組大鼠完全相同。所有操作均需在無(wú)菌環(huán)境下進(jìn)行,手術(shù)前后無(wú)需使用任何抗生素。 3.3測(cè)得各實(shí)驗(yàn)組大鼠對(duì)電子VON FERY測(cè)痛儀所產(chǎn)生機(jī)械刺激的反應(yīng)閾值。 3.4制備ION組織標(biāo)本和Vc組織標(biāo)本。 分別稱取各實(shí)驗(yàn)組大鼠的體重,按體重腹腔注10%水合氯醛(350mg/kg)麻醉后,取結(jié)扎區(qū)ION進(jìn)行組織病理學(xué)觀察(weil氏和HE染色)。Vc組織的定位按照Paxinos等制定的大鼠腦立體定位圖譜進(jìn)行定位,切取定好位后的Vc組織,將標(biāo)記好后的各實(shí)驗(yàn)組大鼠的Vc組織放入冰箱保存(-80℃)。 3.5提取Vc組織抗原蛋白 取出上述標(biāo)記好的各實(shí)驗(yàn)組大鼠的Vc組織塊用PBS液沖洗,將Vc組織放置于冰上用干凈的剪刀盡量剪碎,再將其放入勻漿器內(nèi),每5mg加入300μl配制好的細(xì)胞裂解緩沖液后,裂解30min后,用移液槍移至1.5ml空離心管里,隨后離心5min(12000rpm、低于4℃),將上清液取出之后小心放入已經(jīng)準(zhǔn)備好的空離心管里,然后置入冰箱冷凍儲(chǔ)存(-20℃)。 3.6測(cè)定Vc組織上清液蛋白含量 3.7CBl受體的Western blotting檢測(cè) 4、統(tǒng)計(jì)方法與統(tǒng)計(jì)軟件 采用SPSS13.0統(tǒng)計(jì)軟件對(duì)本實(shí)驗(yàn)的數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析,使用均數(shù)±標(biāo)準(zhǔn)差(x±s)來(lái)表達(dá)實(shí)驗(yàn)研究結(jié)果,單因素方差分析(One-Way ANOVA)和重復(fù)測(cè)量方差分析(Repeated Measures)用于多組間比較,首先進(jìn)行方差齊性檢驗(yàn),如方差不齊,多重比較采用Dunnett's進(jìn)行檢驗(yàn)；如方差齊性,多重比較使用Bonferroni進(jìn)行檢驗(yàn)；P0.05表示差異有統(tǒng)計(jì)學(xué)意義。兩組間比較用配對(duì)樣本t檢驗(yàn)分析(Paired-Samples T Test),P0.05表示差異有統(tǒng)計(jì)學(xué)意義。 5、結(jié)果 (1)行為學(xué)觀察結(jié)果顯示,正常組和假于術(shù)組每天疼痛閾值比較并無(wú)顯著差異,P均大于0.05。手術(shù)組和對(duì)照組比較術(shù)后疼痛閾值具有顯著差異,P均小于0.05。 (2)組織學(xué)觀察結(jié)果顯示：正常結(jié)構(gòu)消失,術(shù)后縮窄環(huán)區(qū)域神經(jīng)纖維腫脹逐漸明顯；受壓迫神經(jīng)纖維粗細(xì)不一致、分布不均勻、甚至發(fā)生變性,被環(huán)扎的神經(jīng)軸突裸露、稀疏、變細(xì)、厚薄不一、髓鞘正常結(jié)構(gòu)消失,呈現(xiàn)出蟲蝕狀節(jié)段性脫失和空泡樣改變,連續(xù)性中斷,大量出現(xiàn)許旺細(xì)胞。 (3)CBl受體在手術(shù)組(ION-CCI1d組、ION-CCI3d組、ION-CCI7d組和ION-CCI14d組)大鼠Vc組織中的含量表達(dá)依次上調(diào),但正常組和假手術(shù)組大鼠Vc組織中CBl受體含量表達(dá)無(wú)顯著性差異；假手術(shù)組大鼠手術(shù)側(cè)與對(duì)側(cè)Vc組織中CBl受體含量表達(dá)無(wú)差異：ION-CCl14d組手術(shù)側(cè)與對(duì)側(cè)Vc組織中CBl受體含量表達(dá)有顯著差異。6、結(jié)論 (1)外周神經(jīng)損傷可呈時(shí)間依賴的形式導(dǎo)致Vc組織中CBl受體表達(dá)上調(diào)。 (2) ION-CCI后誘導(dǎo)了其同側(cè)和對(duì)側(cè)CBl受體表達(dá)增加,在CCI側(cè)CBl受體表達(dá)上調(diào)的程度顯著大于其對(duì)側(cè)。 (3)CBl受體表達(dá)變化與周圍神經(jīng)疼痛閾值變化無(wú)直接關(guān)聯(lián)。
[Abstract]:1, the background
Cannabis is one of the earliest known addictive plants in human beings. The record of using cannabis to stop pain can be traced back to hundreds of years. In the last decade, with the discovery of endogenous endogenous cannabinoids (EC), and two endogenous endogenous receptors (cannabinoid type1receptor, CB1 and cannabinoid type2receptor, C) The existence of the successful cloning of B2, the existence of endocannabinoid system (ECS), has preliminarily proved.CBl, CB2 receptors are G protein coupled receptors, CBl receptors are composed of 473 amino acids and 7 transmembrane domains. The study found that CBl receptors are mainly distributed in the nervous system, mainly in brain tissue, concentrated in the brain. The hippocampus, cerebellum and striatum, mostly located at the nerve terminal presynaptic membrane, play a role in regulating the release of neurotransmitters. In the adrenal, heart, lung, prostate, spleen and tonsillar, the.CB2 receptors are also expressed in 360 amino acids and 7 transmembrane domains, contrary to the CBl receptor, and the CB2 receptor is in central neurons. The concentration is very low, which mainly exists in the immune system, including the spleen, T cells, tonsillar, B cells and mononuclear cells, which mainly play the role of immunoregulation, and can inhibit the release of various neurotransmitters and cytokines. Studies have shown that CBl receptor antagonists can enhance the sensitivity to harmful stimuli and enhance the pain. In addition, CBl receptor agonists can inhibit the excitability of pain neurons and produce good analgesic effects. Related studies also suggest that CBl receptors can reduce nociceptive pain and inhibit spontaneous pain related behavior.
Primary trigeminal neuralgia (Trigeminal neuralgia, TN) is a chronic neuropathic disease, a recurrent knife - cut paroxysmal pain in the branching area of the trigeminal nerve, characterized by transient and severe pain of the trigeminal nerve in the oral and maxillofacial branches or in several branches of the branch, and for a long time Fixed in a branch, especially second, third more common, also several simultaneous involvement, the duration of pain for a few seconds to several minutes, and the intermittent period can be without any symptoms, its hair as spontaneous or from the face, a slight tactile stimulation in the mouth, the majority of the onset of the onset, among them, the elderly good hair. Currently, TN The pathogenesis is still not clear, the exact animal model is still difficult to establish. Most scholars at home and abroad believe that the main factors of TN are the surrounding factors, and the theory of vascular compression has been favored by most scholars, so the animal model of TN is basically through the orbital nerve (infraorbital branch of the trigeminal nerve, ION) ring ligation. To simulate the phenomenon of vascular compression, and to conduct mechanical stimulation on the surface of the ION domination area to simulate the TN trigger point.
The Spinal trigeminal caudal subnucleus (Vc) is divided into five layers from outer to inner. In the medial and lateral medulla of the trigeminal spinal tract, the lateral.Vc is the first gateway to the center of the oral and maxillofacial tissue. The caudal subnucleus and its adjacent area are the primary sensory center of the somatosensory, passing the oral cavity. The nociceptive stimulus information of the maxillofacial region, the caudal subnucleus of.Vc, is equivalent to the posterior horn of the spinal cord in the cellular architecture, which is also considered to be the region of the two stage neurons of the facial nociceptive transmission pathway, which is closely related to the transmission and modulation of the algesia impulse and the degeneration of the gelatinous intermediate neurons in the layer II of.Vc. The modulation of the afferent pain stimulus has lost its effect on the afferent impulse, and the afferent impulse can quickly reach a certain sum and cause severe pain attacks. Therefore, we use Vc tissue to detect the changes in the expression of the CBl receptor.
Some studies have shown that the peripheral nerve of the injured rat and the monkey has a decrease in the expression of micron opioid receptor in the spinal and dorsal root ganglion neurons. The expression of the CBl receptor in the superficial spinal cord of the same lateral dorsal horn of the rat model of the sciatic nerve (CCI) and the expression of the CBl receptor in the lateral thalamus region after the injury of the unilateral nerve of the rat .Nomura and our research team also found that Fos and cdk5/p35 protein like immunoreactivity can be induced in both sides of the Vc tissue in the nasal mouth of the rat with unilateral inferior alveolar nerve, and the opposite side of the transection is less than the transverse.Vc as a sensory relay. The mechanism of the up regulation of CBl receptor expression after CCI is still unknown, but in this model, the expression of the CBl receptor in the spine may be regulated by protein kinase C, tyrosine kinase receptor and related intracellular mitogen activated protein kinase. Pathological features are similar to the pathological features of the rat model of chronic suborbital nerve compression (chronic constriction injury on the infraorbital branch of the trigeminal nerve, ION-CCI).
In this study, a rat model of trigeminal neuralgia was prepared and the changes in the expression of CBl receptor in the Vc tissues of three trigeminal neuralgia rats were detected in this experiment.
2, the purpose
We assume that the neurons in the Vc tissue of the TN rat model are stored in the CBl receptor, because there is still no discussion on how CBl receptors play a role in the TN rat model. In addition, the neurons in the Vc tissue of the lower medulla accept the related information of the sensory nerve conduction of the trigeminal nerve. So, we set the experimental model of the TN. For the rat model of ION-CCI, the changes in the expression of CBl receptor in the Vc tissues of rats after ION-CCI were examined. The aim is to lay a foundation for the study of the clinical treatment of CBl receptor and the association of the pathogenesis of TN.
3, materials and methods
3.1 group of experimental animals
The same batch of healthy adult male SD rats, with a weight of 300-350g, were divided into 6 groups randomly, with 6 rats in each group, which were divided into normal group, sham operation group, group ION-CCI1d, group ION-CCI3d, ION-CCI7d group, group ION-CCI14d. The male SD rats were fed by the laboratory animal laboratory of Zhujiang Hospital of Southern Medical University, the temperature was controlled at about 25, and the humidity was 4 Between 5%-50%, 12h and dark cycle (07:00 - 19:00). In order to eliminate the interference of the randomness of animal behavior to the experimental process and results, reduce the resulting false positive results. Before the start of the experiment, each 08:00 begins with a homemade plastic rod knocking around the cage wall and caged top, touching, lifting the rat, after the rat habit, to be big. When the rat was calm, a self-made brush was used to stimulate the rat's mattress. 3 times / each side, each stimulus should be 2 times, and the two time interval should not be less than 30 seconds. The two sides were alternately carried out, which directly caused the rat from the beginning of the nose, the inquiry, the fear, even the quick retreat escape, and the bite attack and other tranquility.
3.2 exposure and parallel supraorbital nerve ligation
The experimental animals were intraperitoneally injected with 10% chloral chloral (350mg/kg). The rats with satisfactory anesthesia were supine and fixed on the head and limbs. Along the edge of the cheek of the first molar of the right side, the incision was cut longitudinally for 1cm in the direction of the mouth and nose. The ION and the surrounding 5mm were separated, and the two between the eyes and the microscope were used between the eyes and the microscope. The 5 chromic intestinal line from about 2mmm was loosely ligated ION, the tension of ligation of ION was required to reduce the diameter of the ION, delay the ION conduction, not completely block the blood conduction of the blood vessels of the nerve surface, and the blood circulation must be smooth. The 6 suture line sutured the wound after operation. The rats in the sham operation group were in addition to the ligation of ION, and the other aspects were finished with the operation group. All operations are performed in sterile environment. No antibiotics are needed before and after operation.
3.3 the response threshold of each experimental group to the mechanical stimulus produced by the electronic VON FERY pain analyzer was measured.
3.4 the specimens of ION tissue and Vc tissue were prepared.
The body weight of the rats in the experimental group was taken respectively, and after anaesthesia was injected into the abdominal cavity of 10% chloral chloral (350mg/kg), the ION of the ligation area was taken for histopathological observation (Weil's and HE staining), and the location of the.Vc tissue in the rat brain was located according to Paxinos and so on, and the Vc tissue after the fixed position was cut and the experimental groups were marked. The Vc tissues of the rats were stored in the refrigerator (-80 C).
3.5 extraction of Vc tissue antigen protein
The Vc tissues of the rats in the experimental groups were flushed with PBS liquid, and the Vc tissues were placed on the ice with a clean scissors, and then put into the homogenizer. After each 5mg was added to the 300 mu l buffer solution, the cracking 30min was transferred to the 1.5ml centrifuge tube with a graving gun and then centrifuged 5min (12000rpm, less than 4). After removing the supernatant, carefully put it into the empty centrifuge tube and store it in the fridge for storage (-20 C).
3.6 determination of protein content in the supernatant of Vc tissue
Detection of 3.7CBl receptor by Western blotting
4, statistical method and statistical software
The data of this experiment were statistically analyzed with SPSS13.0 statistical software, and the experimental results were expressed using mean mean deviation standard deviation (x + s). Single factor variance analysis (One-Way ANOVA) and repeated measurement variance analysis (Repeated Measures) were used for multigroup comparison. First, the variance homogeneity test was carried out, such as variance inhomogeneous, multiple comparison using Dunn. Ett's was tested, such as homogeneity of variance, multiple comparison using Bonferroni to test, P0.05 indicated that the difference was statistically significant. The two groups were compared with the paired sample t test analysis (Paired-Samples T Test), P0.05 indicated that the difference was statistically significant.
5, the result
(1) the results of behavioral observation showed that there was no significant difference in the threshold of pain between the normal group and the false group. The P was greater than that in the 0.05. operation group and the control group, and the pain threshold was significantly different after the operation, and the P was less than 0.05..
(2) the histological observation showed that the normal structure disappeared and the nerve fiber swelling in the constrictive ring region was gradually obvious. The compressed nerve fibers were not uniform, uneven, even denatured, and the axons were exposed, thinned, thin, and thin, and the normal structure of myelin disappeared. Vacuolar changes, continuity interruption, a large number of Schwann cells.
(3) the content expression of CBl receptor in the Vc tissue of the operation group (group ION-CCI1d, ION-CCI3d, ION-CCI7d and ION-CCI14d) was up, but there was no significant difference in the expression of CBl receptor in the normal group and the sham operation group, and there was no difference in the expression of the CBl receptor content in the operative side and the contralateral Vc tissue in the sham operation group: ION-CCl1 There was a significant difference in the expression of CBl receptor between the operative side and contralateral Vc tissue in group 4D,.6
(1) peripheral nerve injury can be expressed in a time-dependent manner, resulting in up regulation of CBl receptor expression in Vc tissues.
(2) after ION-CCI, the expression of CBl receptor on ipsilateral and contralateral side was increased, and the expression of CBl receptor on CCI side was significantly higher than that in contralateral side.
(3) there was no direct correlation between the expression of CBl receptor and peripheral nerve pain threshold.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R-332

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