本文選題：腹膜纖維化 + 腹膜透析��； 參考：《山東醫(yī)藥》2017年37期

【摘要】：目的構(gòu)建腹膜纖維化大鼠模型,探討其腹膜組織中熱休克蛋白(HSP27、HSP70、HSP90)的表達變化。方法將40只SD大鼠隨機均分為空白對照組及造模2周組、造模4周組、造模6周組。模型組每日給予4.25%葡萄糖持續(xù)非臥床性腹膜透析液(100 m L/kg)腹腔注射,建立腹膜纖維化大鼠模型,空白對照組每日給予等量的生理鹽水腹腔注射。各組于末次腹膜透析后48 h行腹膜平衡試驗評估腹膜轉(zhuǎn)運功能(超濾量、小分子溶質(zhì)轉(zhuǎn)運率);采集大鼠腹膜組織,用HE染色法和Masson染色法觀察其組織病理學變化;采用免疫組化法檢測大鼠腹膜組織TGF-β1、Smad2/3、HSP27、HSP70、HSP90蛋白表達。結(jié)果隨腹膜透析時間延長,模型組大鼠腹膜超濾量逐漸降低,小分子溶質(zhì)轉(zhuǎn)運率逐漸升高(P均0.05);腹膜逐漸增厚,間質(zhì)有炎癥細胞浸潤伴水腫及毛細血管增生;腹膜基質(zhì)層增厚,膠原纖維增多,各組大鼠腸系膜組織厚度、腹膜組織厚度比較差異有統(tǒng)計學意義(P均0.05)。各組大鼠腹膜組織TGF-β1、Smad2/3、HSP27、HSP70、HSP90蛋白表達比較差異有統(tǒng)計學意義(P均0.05)。結(jié)論成功構(gòu)建腹膜纖維化大鼠模型;其腹膜組織中HSP27、HSP70、HSP90蛋白呈高表達,且透析時間越長,以上指標表達變化越明顯。
[Abstract]:Objective to establish a rat model of peritoneal fibrosis and to investigate the expression of heat shock protein (HSP27) HSP70 (HSP90) in peritoneal tissues of rats. Methods Forty Sprague-Dawley rats were randomly divided into blank control group, 2 week model making group, 4 week model making group and 6 week model making group. The model group was given 4.25% glucose continuously intraperitoneal injection of 100 mL / kg peritoneal dialysate (100 mL / kg) daily to establish the model of peritoneal fibrosis, and the blank control group was given the same amount of normal saline daily intraperitoneal injection. Peritoneal balance test was performed 48 hours after the last peritoneal dialysis to evaluate peritoneal transport function (ultrafiltration, small molecule solute transport rate), peritoneal tissues were collected and histopathological changes were observed by HE staining and Masson staining. The expression of TGF- 尾 1 Smad2 / 3 HSP27HSP70 HSP90 protein in rat peritoneal tissues was detected by immunohistochemical method. Results with the prolongation of peritoneal dialysis time, the peritoneal ultrafiltration decreased gradually, the transport rate of small molecule solute increased gradually, the peritoneal membrane became thicker, the interstitial inflammatory cells infiltrated with edema and capillary hyperplasia, and the peritoneal matrix layer thickened. There were significant differences in the thickness of mesenteric tissue and peritoneal tissue between each group (P < 0.05). There were significant differences in the expression of TGF- 尾 1 Smad2 / 3 HSP27, HSP70, HSP90 and HSP90 in the peritoneal tissues of rats in each group (P < 0.05). Conclusion the rat model of peritoneal fibrosis was successfully constructed, and the expression of HSP27, HSP70, HSP90 was higher in peritoneal tissue, and the longer the dialysis time was, the more obvious the expression of HSP27, HSP70 and HSP90 was.
【作者單位】： 昆明醫(yī)科大學第一附屬醫(yī)院;
【基金】：中國醫(yī)院協(xié)會血液凈化中心管理分會研究項目(CHABP2016-07)
【分類號】：R-332;R459.5;R572.2

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