本文選題：內皮細胞 + 高尿酸血癥　； 參考：《青島大學》2012年碩士論文

【摘要】：目的：通過觀察人血管內皮細胞在不同濃度尿酸鹽刺激后,相關炎癥因子表達的變化,探討尿酸鹽對人血管內皮細胞炎性損傷的機制。方法：體外培養(yǎng)人血管內皮細胞,分別用含尿酸鹽濃度為720μ mol/L(高2)、540u mo1/L(高)、420μmol/L(中)、300μ mol/L(低1)、180μ mol/L(低2)的培養(yǎng)液刺激24h,對照組不加尿酸她刺激,用酶聯免疫吸附雙抗體夾心法(ELISA)測定上清液單核細胞趨化蛋白-1(MCP-1)、細胞間粘附分子-1(ICAM-1)、血管細胞粘附分子-1(VCAM-1)表達水平,用逆轉錄-聚合酶鏈式反應(RT-PCR)技術測定上述3種因子mRNA的表達水平。結果：①MCP-1：上清液中對照組與高2、高1濃度組比較,結果有顯著差異(P＜0.05),中濃度組與高2濃度組比較,結果有顯著差異(P＜0.05),高2與高1濃度組比較,結果有顯著差異(P＜0.05),mRNA水平上對照組與高2、高1、中濃度組比較結果有顯著差異(P＜0.05)；②ICAM-1:上清液中對照組與高2、高1濃度組比較,結果有顯著差異(P＜0.05),中濃度組與高2、高1濃度組比較,結果有顯著差異(P＜0.05),mRNA水平上對照組與高2、高1、中濃度組比較結果有顯著差異(P＜0.05)；③VCAM-1:土青液和mRNA水平對照組與其他各組比較均無顯著差異(P＞0.05)。結論：尿酸鹽可以直接激活內皮細胞,引起相關炎癥因子mRNA及蛋白水平的升高,誘發(fā)炎癥反應。
[Abstract]:Aim: to investigate the mechanism of inflammatory injury induced by uric acid in human vascular endothelial cells (HUVEC) by observing the expression of related inflammatory factors after stimulation with different concentrations of uric acid. Methods: human vascular endothelial cells were cultured in vitro. The cultured human vascular endothelial cells were stimulated with 720 渭 mol / L uric acid at a concentration of 720 渭 mol / L (540u / L) (420 渭 mol / L) (300 渭 mol / L) (180 渭 mol / L (low 2) for 24 hours, while those in the control group were not stimulated by uric acid. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of monocyte chemoattractant protein (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) in supernatant. Reverse transcription-polymerase chain reaction (RT PCR) was used to detect the expression of mRNA. Results in the supernatant, there were significant differences between the control group and the high concentration group (P < 0.05), the middle concentration group compared with the high 2 concentration group (P < 0.05), the high 2 concentration group compared with the high concentration group (P < 0.05), and the high 2 group compared with the high concentration group (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). The results of the middle concentration group were significantly higher than those of the control group (P < 0.05) and the high concentration group (P < 0.05). The results of the supernatant medium group were significantly different from those of the control group (P < 0.05) and the high concentration group (P < 0.05), and the comparison of the middle concentration group with the high concentration group (2%) and the high concentration group (1) showed significant difference (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). There was significant difference between the control group and the control group (P < 0.05). There was no significant difference between the control group and other groups in the level of Tu Qing liquid and mRNA (P > 0.05). Conclusion: uric acid can directly activate endothelial cells, increase the level of mRNA and protein, and induce inflammatory reaction.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R363

【共引文獻】

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