本文選題：內(nèi)皮細(xì)胞 + 高尿酸血癥��； 參考：《青島大學(xué)》2012年碩士論文

【摘要】：目的：通過(guò)觀察人血管內(nèi)皮細(xì)胞在不同濃度尿酸鹽刺激后,相關(guān)炎癥因子表達(dá)的變化,探討尿酸鹽對(duì)人血管內(nèi)皮細(xì)胞炎性損傷的機(jī)制。方法：體外培養(yǎng)人血管內(nèi)皮細(xì)胞,分別用含尿酸鹽濃度為720μ mol/L(高2)、540u mo1/L(高)、420μmol/L(中)、300μ mol/L(低1)、180μ mol/L(低2)的培養(yǎng)液刺激24h,對(duì)照組不加尿酸她刺激,用酶聯(lián)免疫吸附雙抗體夾心法(ELISA)測(cè)定上清液?jiǎn)魏思?xì)胞趨化蛋白-1(MCP-1)、細(xì)胞間粘附分子-1(ICAM-1)、血管細(xì)胞粘附分子-1(VCAM-1)表達(dá)水平,用逆轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)技術(shù)測(cè)定上述3種因子mRNA的表達(dá)水平。結(jié)果：①M(fèi)CP-1：上清液中對(duì)照組與高2、高1濃度組比較,結(jié)果有顯著差異(P＜0.05),中濃度組與高2濃度組比較,結(jié)果有顯著差異(P＜0.05),高2與高1濃度組比較,結(jié)果有顯著差異(P＜0.05),mRNA水平上對(duì)照組與高2、高1、中濃度組比較結(jié)果有顯著差異(P＜0.05)；②ICAM-1:上清液中對(duì)照組與高2、高1濃度組比較,結(jié)果有顯著差異(P＜0.05),中濃度組與高2、高1濃度組比較,結(jié)果有顯著差異(P＜0.05),mRNA水平上對(duì)照組與高2、高1、中濃度組比較結(jié)果有顯著差異(P＜0.05)；③VCAM-1:土青液和mRNA水平對(duì)照組與其他各組比較均無(wú)顯著差異(P＞0.05)。結(jié)論：尿酸鹽可以直接激活內(nèi)皮細(xì)胞,引起相關(guān)炎癥因子mRNA及蛋白水平的升高,誘發(fā)炎癥反應(yīng)。
[Abstract]:Aim: to investigate the mechanism of inflammatory injury induced by uric acid in human vascular endothelial cells (HUVEC) by observing the expression of related inflammatory factors after stimulation with different concentrations of uric acid. Methods: human vascular endothelial cells were cultured in vitro. The cultured human vascular endothelial cells were stimulated with 720 渭 mol / L uric acid at a concentration of 720 渭 mol / L (540u / L) (420 渭 mol / L) (300 渭 mol / L) (180 渭 mol / L (low 2) for 24 hours, while those in the control group were not stimulated by uric acid. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of monocyte chemoattractant protein (MCP-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) in supernatant. Reverse transcription-polymerase chain reaction (RT PCR) was used to detect the expression of mRNA. Results in the supernatant, there were significant differences between the control group and the high concentration group (P < 0.05), the middle concentration group compared with the high 2 concentration group (P < 0.05), the high 2 concentration group compared with the high concentration group (P < 0.05), and the high 2 group compared with the high concentration group (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). The results of the middle concentration group were significantly higher than those of the control group (P < 0.05) and the high concentration group (P < 0.05). The results of the supernatant medium group were significantly different from those of the control group (P < 0.05) and the high concentration group (P < 0.05), and the comparison of the middle concentration group with the high concentration group (2%) and the high concentration group (1) showed significant difference (P < 0.05). Results there was significant difference in mRNA level between the control group and the control group (P < 0.05). There was significant difference between the control group and the control group (P < 0.05). There was no significant difference between the control group and other groups in the level of Tu Qing liquid and mRNA (P > 0.05). Conclusion: uric acid can directly activate endothelial cells, increase the level of mRNA and protein, and induce inflammatory reaction.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R363

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

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