本文選題：血小板低下 + 樹(shù)突狀細(xì)胞��； 參考：《中國(guó)實(shí)驗(yàn)血液學(xué)雜志》2017年01期

【摘要】：目的:探討血小板低下對(duì)過(guò)繼性樹(shù)突狀細(xì)胞(DC)在體內(nèi)遷移模式的影響。方法:腹腔注射抗CD41單克隆抗體以建立血小板低下小鼠模型。將帶報(bào)告基因的骨髓來(lái)源DC經(jīng)腳墊和靜脈兩種途徑輸注至血小板低下和正常小鼠體內(nèi),用活體成像技術(shù)監(jiān)測(cè)DC在小鼠體內(nèi)的遷移和組織內(nèi)分布情況。結(jié)果:經(jīng)抗GPⅡb抗體處理組小鼠體內(nèi)80%以上血小板被清除。DC經(jīng)腳墊回輸72 h后,在血小板低下組遷移到乆窩淋巴結(jié)(PLN)和腹股溝淋巴結(jié)(ILN)的比例分別為(0.32±0.02)%和(0.02±0.01)%;對(duì)照組上述兩部位內(nèi)為(0.27±0.15)%和(0.02±0.02)%。兩組之間無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)。經(jīng)靜脈輸注DC 72 h后,血小板低下組和對(duì)照組小鼠組織內(nèi)的分布均主要集中在脾臟、肝臟引流淋巴結(jié)、肺臟和肝臟等,且分布比例無(wú)統(tǒng)計(jì)學(xué)差異。結(jié)論:小鼠體內(nèi)血小板低下至不足20%時(shí),對(duì)經(jīng)皮下和靜脈兩種途徑輸注的DC在體內(nèi)遷移和組織分布無(wú)明顯影響。
[Abstract]:Aim: to investigate the effect of thrombocytopenia on the migration pattern of adoptive dendritic cells (DC) in vivo. Methods: mouse model of thrombocytopenia was established by intraperitoneal injection of anti-CD41 monoclonal antibody. DC derived from bone marrow with reporter gene was infused into mice with thrombocytopenia and normal mice via foot pad and vein. The migration and distribution of DC in vivo were monitored by in vivo imaging. Results: more than 80% of platelets were cleared in mice treated with anti-GP 鈪，

本文編號(hào)：1804657