本文選題：腦缺血-再灌注 + 骨髓間充質(zhì)干細(xì)胞��； 參考：《國(guó)際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志》2016年02期

【摘要】：目的觀察骨髓間充質(zhì)干細(xì)胞(BMSCs)移植對(duì)缺血再灌注損傷后大鼠腦組織miR-34a和survivin表達(dá)的影響,探討B(tài)MSCs移植的抗凋亡和神經(jīng)保護(hù)作用機(jī)制。方法將192只大鼠隨機(jī)分為空白組、模型組、PBS液移植組和干細(xì)胞移植組;采用改良Longa線栓法制作大鼠大腦中動(dòng)脈閉塞再灌注(MCAO)模型;通過(guò)尾靜脈注射法行干細(xì)胞移植;改良大鼠神經(jīng)功能缺損評(píng)分(m NSS)評(píng)估神經(jīng)功能缺損;免疫組化檢測(cè)survivin的表達(dá);實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)miR-34a的表達(dá)。結(jié)果干細(xì)胞移植組的神經(jīng)功能缺損評(píng)分在12 h、1 d時(shí)與模型組比較無(wú)明顯差異(P0.05);3 d、7 d時(shí)明顯低于模型組(P0.05)。干細(xì)胞移植組的survivin陽(yáng)性細(xì)胞率在各時(shí)間點(diǎn)均顯著高于模型組(P0.05)。干細(xì)胞移植組的miR-34 a表達(dá)量在各時(shí)間點(diǎn)均顯著低于模型組(P0.01)。結(jié)論大鼠腦缺血-再灌注損傷可致病灶區(qū)miR-34 a的表達(dá)上調(diào);干細(xì)胞移植可明顯改善腦缺血-再灌注大鼠的神經(jīng)功能;移植干細(xì)胞可能通過(guò)下調(diào)病灶區(qū)miR-34a和上調(diào)survivin的表達(dá)發(fā)揮抗凋亡及神經(jīng)保護(hù)作用。
[Abstract]:Objective to observe the effect of bone marrow mesenchymal stem cell transplantation (BMSCs) on the expression of miR-34a and survivin in rat brain after ischemia-reperfusion injury and to explore the mechanism of anti-apoptosis and neuroprotective effect of BMSCs transplantation. Methods 192 rats were randomly divided into blank group (model group) and stem cell transplantation group (MCAO) model of middle cerebral artery occlusion (MCAO) was established by modified Longa thread occlusion (MCAO) method, and stem cell transplantation was performed by caudal vein injection. The neural function defect was evaluated by modified neurologic impairment score (survivin), the expression of survivin was detected by immunohistochemistry, and the expression of miR-34a was detected by real-time fluorescence quantitative PCR. Results there was no significant difference in neurological deficit score between the stem cell transplantation group and the model group at 12 h and 1 d after transplantation compared with the model group. The score of neural function defect in the stem cell transplantation group was significantly lower than that in the model group at 3 days and 7 days. The percentage of survivin positive cells in stem cell transplantation group was significantly higher than that in model group at each time point. The expression of miR-34 a in stem cell transplantation group was significantly lower than that in model group at each time point. Conclusion the expression of miR-34 a in focal area can be up-regulated by cerebral ischemia-reperfusion injury in rats, and the neural function of rats with cerebral ischemia-reperfusion can be improved by stem cell transplantation. Transplantation of stem cells may play an antiapoptotic and neuroprotective role by down-regulating miR-34a and up-regulating the expression of survivin.
【作者單位】： 南華大學(xué)附屬第二醫(yī)院神經(jīng)內(nèi)科;
【基金】：湖南省科技廳計(jì)劃項(xiàng)目(2012FJ3104)
【分類號(hào)】：R743;R-332

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