本文選題：脊髓損傷 + 慢性壓迫　； 參考：《中國(guó)脊柱脊髓雜志》2017年03期

【摘要】：目的 :探討缺氧誘導(dǎo)因子-1α(hypoxia inducible factor-1α,HIF-1α)和基質(zhì)金屬蛋白酶-2(matrix metalloproteinases-2,MMP-2)在慢性壓迫性頸脊髓損傷大鼠模型中的表達(dá)及意義。方法 :將80只成年SD大鼠隨機(jī)分為假手術(shù)組和慢性壓迫性頸脊髓損傷組(脊髓壓迫組),每組40只。大鼠麻醉后充分顯露C5和C6椎板,切除C5左側(cè)半椎板,顯露硬脊膜,脊髓壓迫組在C6椎板和硬脊膜之間置入吸水后可膨脹聚氨酯薄板(1×3×1mm);假手術(shù)組只顯露硬脊膜不置入壓迫物。兩組分別通過BBB(Basso Beattie Bresnahan)評(píng)分和體感誘發(fā)電位(somatosensory evoked potential,SEP)檢測(cè)評(píng)估脊髓功能;于造模后7d、28d、42d和70d時(shí)處死大鼠取頸脊髓組織進(jìn)行HIF-1α及MMP-2免疫組化染色,檢測(cè)其在各時(shí)間點(diǎn)的表達(dá)量(IOD值),采用獨(dú)立樣本t檢驗(yàn)比較兩組各時(shí)間點(diǎn)的差異,分析HIF-1α、MMP-2與脊髓功能變化的相關(guān)性。結(jié)果:造模后7d時(shí),兩組BBB評(píng)分無顯著性差異;SEP潛伏期顯著性延長(zhǎng),波幅顯著性降低;HIF-1α顯著性降低,MMP-2顯著性升高。28d時(shí)脊髓壓迫組BBB評(píng)分顯著性低于假手術(shù)組,SEP潛伏期與7d比較顯著性延長(zhǎng)(P0.05)、波幅顯著性降低(P0.05),HIF-1α表達(dá)顯著性增高(P0.05),而MMP-2表達(dá)顯著性降低(P0.05)。42d時(shí),脊髓壓迫組BBB評(píng)分、SEP潛伏期和波幅與28d時(shí)比較無顯著性差異,HIF-1α表達(dá)顯著性增高(P0.05),而MMP-2表達(dá)顯著性降低(P0.05);70d時(shí)脊髓壓迫組神經(jīng)功能較28d時(shí)顯著性改善,BBB評(píng)分顯著性增高(P0.05),SEP潛伏期顯著性縮短(P0.05)、波幅顯著性升高(P0.05);HIF-1α表達(dá)較前顯著性降低,而MMP-2表達(dá)升高,但與假手術(shù)組比較仍有顯著性差異(P0.05)。HIF-1α表達(dá)量與BBB評(píng)分呈顯著性負(fù)相關(guān)(r=-0.458,P0.05),MMP-2表達(dá)量與BBB評(píng)分呈顯著性正相關(guān)(r=0.903,P0.05)。結(jié)論:大鼠慢性壓迫性脊髓損傷后具有一定程度的自我修復(fù)能力,HIF-1α、MMP-2表達(dá)變化與慢性脊髓壓迫性損傷后神經(jīng)功能改善相關(guān)。
[Abstract]:Objective: to investigate the expression and significance of hypoxia inducible factor-1 偽 (HIF-1 偽) and matrix metalloproteinases-2 (MMP-2) in chronic compressive cervical spinal cord injury (CSCI) rats. Methods: 80 adult SD rats were randomly divided into sham operation group and chronic compression cervical spinal cord injury group (40 rats in each group). After anesthesia, C5 and C6 laminae were fully exposed, the left half lamina of C5 was excised and the dura dural was exposed. In the spinal cord compression group, 1 脳 3 脳 1mm-1 mm expandable polyurethane thin plate was placed between the C6 lamina and the dura mater, and the sham operation group only exposed the dura mater not placed into the compression material. The spinal cord function was evaluated by BBB(Basso Beattie Bresnahan score and somatosensory evoked potentialsep, and the cervical spinal cord tissues were sacrificed for HIF-1 偽 and MMP-2 immunohistochemical staining on the 7th day, 28d, 42d and 70d, respectively. The expression of HIF-1 偽 MMP-2 was measured at different time points, and the correlation between HIF-1 偽 MMP-2 and spinal cord function was analyzed by t test. Results: there was no significant difference in BBB scores between the two groups at 7 days after modeling. The BBB score of spinal cord compression group was significantly lower than that of sham operation group (P 0.05), and the amplitude was significantly lower than that of sham operation group (P 0.05), while the expression of HIF-1 偽 in spinal cord compression group was significantly increased (P 0.05) and MMP-2 was significantly higher than that of sham operation group (P < 0.05), and the amplitude was significantly lower than that of sham operation group (P < 0.05), while the expression of HIF-1 偽 in spinal cord compression group was significantly higher than that in sham operation group (P < 0.05). The expression of P0. 05 and P0. 05 was significantly decreased on day 42, and the expression of P0. 05 was significantly decreased. There was no significant difference in latency and amplitude of BBB between spinal cord compression group and 28d group. The expression of HIF-1 偽 was significantly increased in spinal cord compression group. However, the expression of MMP-2 was significantly decreased at 70 days after spinal cord compression. The neurological function of spinal cord compression group was significantly improved compared with that at 28 days. The latent period of SEP was significantly shortened and the expression of HIF-1 偽 was significantly decreased. The expression of MMP-2 was increased, but there was still significant difference between the expression of P0.05U. HIF-1 偽 and BBB score. There was a significant negative correlation between the expression of MMP-2 and the BBB score. There was a significant positive correlation between the expression of MMP-2 and the BBB score. Conclusion: the expression of HIF-1 偽 and MMP-2 is related to the improvement of neural function after chronic compressive spinal cord injury in rats.
【作者單位】： 中山大學(xué)附屬第一醫(yī)院脊柱外科;廣東省骨科學(xué)重點(diǎn)實(shí)驗(yàn)室;
【分類號(hào)】：R651.2;R-332

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