本文選題：流感病毒 + 實(shí)時(shí)熒光PCR法��； 參考：《醫(yī)學(xué)動(dòng)物防制》2016年05期

【摘要】：目的對(duì)病毒分離培養(yǎng)法、實(shí)時(shí)熒光PCR法和雞胚培養(yǎng)法3種檢測(cè)流感病毒的方法和結(jié)果進(jìn)行比較研究,篩選快速準(zhǔn)確的檢測(cè)方法,為流感監(jiān)測(cè)與防控工作提供及時(shí)可靠的病原學(xué)依據(jù)。方法同時(shí)采用病毒分離培養(yǎng)法、實(shí)時(shí)熒光PCR法和雞胚培養(yǎng)法對(duì)2014年10月~2015年3月牡丹江市流感監(jiān)測(cè)哨點(diǎn)醫(yī)院采集的流感樣病例咽拭子樣本進(jìn)行檢測(cè),比較檢測(cè)結(jié)果。結(jié)果實(shí)時(shí)熒光PCR法的流感病毒核酸檢測(cè)陽性率為15.52%,其中169份為季節(jié)性H3N2流感病毒,73份為B型Yamagata株;病毒分離培養(yǎng)法的陽性率為6.29%,分離出63株季節(jié)性H3N2流感病毒,35株B型Yamagata株;雞胚分離培養(yǎng)法檢出率為0.64%。3種方法中,實(shí)時(shí)熒光PCR法的陽性率最高,病毒分離培養(yǎng)法次之,雞胚分離培養(yǎng)法陽性率最低。結(jié)論實(shí)時(shí)熒光PCR法比病毒分離培養(yǎng)法和雞胚培養(yǎng)法靈敏快捷、特異性強(qiáng)、敏感度高,適用于流感病毒的快速檢測(cè)。
[Abstract]:Objective to compare the three methods and results of virus isolation and culture, real-time fluorescence PCR and chicken embryo culture, and to screen rapid and accurate methods for detection of influenza virus. To provide timely and reliable etiological basis for influenza surveillance and prevention and control. Methods simultaneously, virus isolation culture method, real-time fluorescence PCR method and chicken embryo culture method were used to detect the throat swabs of influenza cases collected from Mudanjiang influenza surveillance sentinel hospital from October 2014 to March 2015, and the results were compared. Results the positive rate of influenza virus nucleic acid detected by real-time fluorescent PCR method was 15.52, of which 169 were seasonal H3N2 influenza virus and 73 were type B Yamagata strain, and the positive rate of virus isolation culture method was 6.29 and 63 seasonal H3N2 influenza virus type B Yamagata strains were isolated. The positive rate of chicken embryo isolation culture method was 0.64.3, the positive rate of real-time fluorescence PCR method was the highest, virus isolation culture method was the second, and chicken embryo isolation culture method had the lowest positive rate. Conclusion the method of real-time fluorescence PCR is more sensitive, specific and sensitive than virus isolation culture and chicken embryo culture. It is suitable for rapid detection of influenza virus.
【作者單位】： 牡丹江市疾病預(yù)防控制中心;
【分類號(hào)】：R373.13;R446.5
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本文編號(hào)：1780825