本文選題：piTreg + 心臟移植��； 參考：《浙江大學(xué)》2011年碩士論文

【摘要】：目的：探討過繼輸注PiTreg/Treg對小鼠心臟移植的保護(hù)作用。 實(shí)驗(yàn)方法：分別以近交系健康雄性Balb/c小鼠和C57BL/6小鼠為供、受者,制備小鼠腹部異位心臟移植模型。受者于術(shù)前接受2Gy照射,術(shù)前1天靜脈輸注相應(yīng)細(xì)胞：對照組輸注200μl生理鹽水；Treg組輸注健康雌性C57BL/6的Treg,5x105/200μl；PiTreg組輸注孕14d C57BL/6的Treg細(xì)胞(即piTreg細(xì)胞,與雄性Balb/c小鼠交配),5x105/200μ1。觀察各組移植心臟的存活時(shí)間和組織病理學(xué)變化；流式分析脾臟淋巴細(xì)胞中Treg細(xì)胞比率、Foxp3的表達(dá)率變化、嵌合體形成等。 結(jié)果：(1)以40d為觀察終點(diǎn),Treg組和PiTreg組移植心臟存活時(shí)間均為40±0d(n=6),較對照組9.67±0.58d(n=3)明顯延長,p0.01(2)術(shù)后10d,Treg組和PiTreg組的病理排斥反應(yīng)較對照組顯著減輕,排斥反應(yīng)不明顯。(3)piTreg組和Treg組兩組小鼠,術(shù)后脾臟細(xì)胞均出現(xiàn)不同程度的嵌合體,其中piTreg組為5.48±1.04%,Treg組1.42±0.28%,p=0.029。(4)piTreg組和Treg組兩組小鼠,術(shù)后脾臟中Treg/CD4+T細(xì)胞的比例、Foxp3/CD4+T細(xì)胞的比例較健康C57/BL小鼠有顯著增加,其中以輸注piTreg增加更顯著。(5)piTreg組和Treg組兩組小鼠血清中RANTES、Mig等趨化因子的含量較對照組明顯降低,但兩組之間(Treg組和piTreg組)差異不明顯。 結(jié)論：在小鼠同種異型心臟移植模型中,過繼輸注piTreg/Treg細(xì)胞能有效延長移植心臟的存活時(shí)間,其機(jī)理可能是形成微嵌合體,上調(diào)外周Treg/CD4+T細(xì)胞比例、Foxp3/CD4+T細(xì)胞比例,減少血清炎癥因子等。
[Abstract]:Objective: to investigate the protective effect of adoptive infusion of PiTreg/Treg on heart transplantation in mice.Methods: heterotopic abdominal heart transplantation models were established by using inbred healthy male Balb/c mice and C57BL/6 mice as donors and recipients respectively.The recipient received 2Gy irradiation before operation, and the corresponding cells were injected intravenously 1 day before operation: the control group was infused with 200 渭 l normal saline treg group, and the healthy female C57BL/6 group was infused with the Treg cells (piTreg cells) of C57BL/6 on the 14th day of gestation in the 5 x 105 / 200 渭 l Pig group of healthy female C57BL/6, and the corresponding cells were mated with male Balb/c mice by 5 x 105 / 200 渭 1.The survival time and histopathological changes of transplanted heart were observed, and the expression rate of Foxp3 and chimerism in splenic lymphocytes were analyzed by flow cytometry.Results the survival time of transplanted heart in PiTreg group and PiTreg group was 40 鹵0 d, which was significantly longer than that in control group (9.67 鹵0.58 d ~ 3). The pathological rejection was significantly reduced in 10 days after operation in the Treg group and the PiTreg group. The rejection was not obvious in the two groups.There were different degrees of chimerism in spleen cells after operation, including 5.48 鹵1.04T cells in piTreg group and 1.42 鹵0.28g group in Treg group. The ratio of Treg/CD4 T cells in the spleen of piTreg group and Treg group was significantly higher than that in healthy C57/BL mice.The levels of serum chemokines such as RANTESS-Mig in the piTreg group and Treg group were significantly lower than those in the control group, but there was no significant difference between the two groups.Conclusion: adoptive infusion of piTreg/Treg cells can effectively prolong the survival time of allogeneic heart transplantation in mice. The mechanism may be the formation of microchimerism and the up-regulation of the ratio of peripheral Treg/CD4 T cells to Foxp3 / CD4 T cells.Reduce serum inflammatory factors and so on.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R392

【共引文獻(xiàn)】

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2 程雁;B淋巴細(xì)胞刺激因子及其受體介導(dǎo)系統(tǒng)性紅斑狼瘡患者B淋巴細(xì)胞活性的作用及TACI-Ig對其的影響[D];安徽醫(yī)科大學(xué);2012年

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2 孔珍珍;兒童過敏性哮喘特異性免疫治療過程中Foxp3和IL-27 mRNA表達(dá)水平的變化[D];鄭州大學(xué);2011年

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本文編號：1759698