本文選題：Exendin- + 聚乙二醇化��； 參考：《中國藥科大學(xué)學(xué)報》2016年02期

【摘要】：基于熒光素酶報告基因法和本實驗室前期構(gòu)建的CHO-GLP-1R-CRE-Luc+檢測模型細胞株,建立并優(yōu)化了長效降糖多肽——聚乙二醇化Exendin-4類似物PE與GLP-1受體的親和力測定方法,并對其進行了方法學(xué)驗證。結(jié)果表明,當(dāng)藥物作用4 h,化學(xué)發(fā)光底物作用15 min,檢測藥物在5.7×10-3~1.5×103nmol/L濃度區(qū)間內(nèi)時,該檢測方法的專屬性和耐用性良好、準(zhǔn)確度和精密度較高,符合《生物制品質(zhì)量控制分析方法驗證技術(shù)一般原則》的相關(guān)要求。本研究為該類GLP-1受體激動劑藥物的快速評價與篩選奠定了基礎(chǔ)。
[Abstract]:Based on the luciferase reporter gene method and the CHO-GLP-1R-CRE-Luc detection model cell line constructed in our laboratory, the affinity of the long-acting hypoglycemic polypeptide-polyethylene glycol Exendin-4 analogue PE to GLP-1 receptor was established and optimized.The methodology was validated.The results showed that the specificity and durability of the method were good, the accuracy and precision were high when the chemiluminescence substrate was exposed to the chemiluminescent substrate for 4 h and the detection drug was in the concentration range of 5.7 脳 10 ~ (-3) ~ (-1) 脳 1. 5 脳 103nmol/L.Accord with the requirements of the General principle of validation Technology for quality Control Analysis of Biological products.This study laid a foundation for rapid evaluation and screening of GLP-1 receptor agonists.
【作者單位】： 中國藥科大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：國家“重大新藥創(chuàng)制”科技重大專項資助項目(No.2012ZX09103101-016) 教育部博士點基金資助項目(No.20120096110007)~~
【分類號】：R392-33

【相似文獻】

相關(guān)期刊論文 前1條

1 袁濤;王智杰;鄧杰;張珂;李征;張雪梅;;PEG-rhIL-6與rhIL-6在化療模型小鼠中體內(nèi)藥效的比較研究[J];微生物學(xué)免疫學(xué)進展;2011年02期

，

本文編號：1757078