本文選題：Exendin- + 聚乙二醇化　； 參考：《中國(guó)藥科大學(xué)學(xué)報(bào)》2016年02期

【摘要】：基于熒光素酶報(bào)告基因法和本實(shí)驗(yàn)室前期構(gòu)建的CHO-GLP-1R-CRE-Luc+檢測(cè)模型細(xì)胞株,建立并優(yōu)化了長(zhǎng)效降糖多肽——聚乙二醇化Exendin-4類似物PE與GLP-1受體的親和力測(cè)定方法,并對(duì)其進(jìn)行了方法學(xué)驗(yàn)證。結(jié)果表明,當(dāng)藥物作用4 h,化學(xué)發(fā)光底物作用15 min,檢測(cè)藥物在5.7×10-3~1.5×103nmol/L濃度區(qū)間內(nèi)時(shí),該檢測(cè)方法的專屬性和耐用性良好、準(zhǔn)確度和精密度較高,符合《生物制品質(zhì)量控制分析方法驗(yàn)證技術(shù)一般原則》的相關(guān)要求。本研究為該類GLP-1受體激動(dòng)劑藥物的快速評(píng)價(jià)與篩選奠定了基礎(chǔ)。
[Abstract]:Based on the luciferase reporter gene method and the CHO-GLP-1R-CRE-Luc detection model cell line constructed in our laboratory, the affinity of the long-acting hypoglycemic polypeptide-polyethylene glycol Exendin-4 analogue PE to GLP-1 receptor was established and optimized.The methodology was validated.The results showed that the specificity and durability of the method were good, the accuracy and precision were high when the chemiluminescence substrate was exposed to the chemiluminescent substrate for 4 h and the detection drug was in the concentration range of 5.7 脳 10 ~ (-3) ~ (-1) 脳 1. 5 脳 103nmol/L.Accord with the requirements of the General principle of validation Technology for quality Control Analysis of Biological products.This study laid a foundation for rapid evaluation and screening of GLP-1 receptor agonists.
【作者單位】： 中國(guó)藥科大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：國(guó)家“重大新藥創(chuàng)制”科技重大專項(xiàng)資助項(xiàng)目(No.2012ZX09103101-016) 教育部博士點(diǎn)基金資助項(xiàng)目(No.20120096110007)~~
【分類號(hào)】：R392-33

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