本文選題：Toll樣受體- + B淋巴細胞刺激因子　； 參考：《免疫學雜志》2017年09期

【摘要】：目的研究Toll樣受體-4(Toll-like receptors-4,TLR-4)與B淋巴細胞刺激因子(B lymphocyte stimulator,BLys)在系統(tǒng)性紅斑狼瘡(systemic lupus erythematosus,SLE)轉基因小鼠模型中的作用及可能機制,為SLE的治療提供新靶點。方法選擇SPF級EB病毒膜抗原BLLF1轉基因雌性小鼠共30只,隨機分成空白對照組、BLys阻斷劑組和TLR-4阻斷劑組各10只,選擇SPF級野生型小鼠為野生對照組。BLys阻斷劑組應用抗BR3單克隆抗體500 mg/d,腹腔注射連續(xù)10 d,TLR-4阻斷劑組應用抗人TLR-4抗體250 mg/d,腹腔注射連續(xù)10 d,野生對照組和空白對照組腹腔注射等量生理鹽水。繼續(xù)喂養(yǎng)10 d后無菌取尾靜脈血,RT-PCR法檢測TLR-4 m RNA水平,ELISA法檢測白細胞介素-6(interleukin-6,IL-6)、IL-17、腫瘤壞死因子-α(tumor necrosis factor alpha,TNF-α)和IL-2水平,金標免疫斑點法檢測抗ds DNA抗體滴度,常規(guī)生化法檢測補體C3(complement 3)、血沉(erythrocyte sedimentation rate,ESR)和C反應蛋白(C-reaction protein,CRP)水平,對小鼠腎臟進行HE和Masson染色。結果野生對照組和BLys阻斷劑組,空白對照組和TLR-4阻斷劑組外周血中單個核細胞(PBMC)的BLys無統(tǒng)計學差異(P0.05);與野生對照組和BLys阻斷劑組比,空白對照組和TLR-4阻斷劑組PBMC的BLys明顯增高(P0.05)。提示EB病毒膜抗原BLLF1轉基因小鼠為SLE模型,BLys阻斷劑可阻斷PBMC的BLys的表達。野生對照組和空白對照組干預前后的TLR-4 m RNA、IL-6、IL-17、TNF-α、IL-2、抗ds DNA抗體、C3、ESR和CRP表達無明顯差異(P0.05);空白對照組、BLys阻斷劑組和TLR-4阻斷劑組干預前外周血TLR-4 m RNA、IL-6、IL-17、TNF-α、抗ds DNA抗體、C3、ESR和CRP表達無明顯差異(P0.05)。干預后,BLys阻斷劑組和TLR-4阻斷劑組的TLR-4 m RNA、IL-6、IL-17、TNF-α、抗ds DNA抗體、C3、ESR和CRP較干預前明顯降低(P0.05),IL-2較干預前明顯增高(P0.05)。與野生對照組比,其余組別的TLR-4 m RNA、IL-6、IL-17、TNF-α、IL-2、C3、ESR和CRP表達干預前后均存在差異(P0.05)。與空白對照組比,BLys阻斷劑和TLR-4阻斷劑組HE和Masson染色均有明顯改善。結論BLys和TLR-4可能是參與SLE的發(fā)生發(fā)展,機理可能與調節(jié)免疫炎癥反應有關。
[Abstract]:Objective to study the role and possible mechanism of Toll like receptor -4 Toll-like receptors-4 (TLR-4) and B lymphocyte stimulating factor B lymphocyte stimulator (BLys4) in systemic lupus erythematosus (SLE) transgenic mice, and to provide a new target for the treatment of SLE.Methods Thirty SPF grade EB virus membrane antigen BLLF1 transgenic female mice were randomly divided into control group (n = 10) and TLR-4 blocker group (n = 10).The wild-type mice of SPF grade were selected as wild control group. BLys antagonist group was treated with anti- monoclonal antibody 500mg / d, intraperitoneal injection of anti-human TLR-4 antibody 250mg / d, intraperitoneal injection of anti-human TLR-4 antibody 250mg / d, wild control group and blank control group.Group A received intraperitoneal injection of the same amount of normal saline.After 10 days of continuous feeding, the levels of TLR-4 m RNA were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in sterile caudal vein. The levels of IL-17, TNF- 偽 and IL-2 in tumor necrosis factor were detected by Elisa, and the titer of anti-DS DNA antibody was detected by immunoblot assay.Routine biochemical method was used to detect the levels of complement C3(complement 3, erythrocyte sedimentation rsr) and C-reactive protein C reaction protein (CRP) in the erythrocyte of mice. The kidneys of mice were stained with HE and Masson.Results there was no significant difference in BLys in peripheral blood mononuclear cells between wild control group and BLys blocker group, blank control group and TLR-4 blocker group. Compared with wild control group and BLys blocker group, the BLys of PBMC in blank control group and TLR-4 blocker group was significantly higher than that in control group and TLR-4 blocker group.It is suggested that EB virus membrane antigen BLLF1 transgenic mice can block the expression of PBMC BLys in SLE model.There was no significant difference in the expression of TLR-4 m RNA-IL-17TNF- 偽 and anti-DS DNA antibody C3TSR and CRP between the wild control group and the blank control group before and after intervention, but there was no significant difference in the expression of TLR-4 m RNA-6IL-17TNF- 偽 and anti-DS DNA antibody C3NSR and CRP between the blank control group and the TLR-4 blocker group before and after intervention.After intervention, the levels of TLR-4 m RNAs, IL-6 and IL-17 TNF- 偽, anti-DS DNA antibody C _ 3C _ 3N _ (2) and CRP in the TLR-4 antagonist group were significantly lower than those before the intervention, and the levels of P0.05 and IL-2 were significantly higher than those before the intervention.Compared with the wild control group, there were significant differences in the expression of TLR-4 m RNA-IL-6, IL-17, TNF- 偽, IL-2C3, ESR and CRP between the other groups before and after intervention (P 0.05).Compared with the blank control group, the HE and Masson staining of the two groups were significantly improved.Conclusion BLys and TLR-4 may be involved in the development of SLE, and the mechanism may be related to the regulation of immune inflammation.
【作者單位】： 山東中醫(yī)藥大學附屬醫(yī)院檢驗科;
【分類號】：R-332;R593.241

【相似文獻】

相關期刊論文 前10條

1 鄧燕飛,盧永德;系統(tǒng)性紅斑狼瘡的顳骨研究[J];國外醫(yī)學.耳鼻咽喉科學分冊;2000年04期

2 任文英;系統(tǒng)性紅斑狼瘡小鼠模型的研究近況[J];免疫學雜志;2003年S1期

3 王靜;葉冬青;;系統(tǒng)性紅斑狼瘡發(fā)生、預后的統(tǒng)計模型分析及建模策略[J];數(shù)理醫(yī)藥學雜志;2006年06期

4 何昊蔚;;系統(tǒng)性紅斑狼瘡患者心理問題的分析[J];中國煤炭工業(yè)醫(yī)學雜志;2008年12期

5 張慧茹;;心理干預對系統(tǒng)性紅斑狼瘡患者焦慮抑郁心理的影響[J];實用醫(yī)技雜志;2008年13期

6 朱小平;劉仁碧;;心理治療對系統(tǒng)性紅斑狼瘡個性心理特征的影響[J];現(xiàn)代醫(yī)藥衛(wèi)生;2009年09期

7 吳厚生,邵洪波;系統(tǒng)性紅斑狼瘡與白細胞介素(文獻綜述)[J];上海免疫學雜志;1991年03期

8 陳金,陸右之,劉志中;系統(tǒng)性紅斑狼瘡患者個性的艾森克量表評定[J];中國心理衛(wèi)生雜志;1991年03期

9 陳金;陸右之;徐濤;;人格、抑郁與系統(tǒng)性紅斑狼瘡病例對照研究[J];瀘州醫(yī)學院學報;1991年03期

10 張奉春，董怡，趙玉華，林禾，姜泉，，趙杰;系統(tǒng)性紅斑狼瘡中樞神經(jīng)系統(tǒng)病變的診斷及治療[J];北京醫(yī)學;1994年05期

相關會議論文 前2條

1 蔡紅;;合并系統(tǒng)性紅斑狼瘡孕產(chǎn)婦的心理調查和分析初探[A];全國婦產(chǎn)科護理學術交流暨專題講座會議論文匯編[C];2003年

2 張亞萍;榮良群;王克勤;;系統(tǒng)性紅斑狼瘡患者心理狀況調查[A];中華醫(yī)學會全國風濕病學年會論文匯編[C];2003年

相關博士學位論文 前1條

1 李敏;A20治療系統(tǒng)性紅斑狼瘡小鼠模型機制研究[D];第三軍醫(yī)大學;2016年

相關碩士學位論文 前2條

1 周小剛;脂質體槲皮素對Pristane誘導的系統(tǒng)性紅斑狼瘡樣小鼠模型的效應[D];鄭州大學;2016年

2 劉姍姍;干擾素調節(jié)因子4血清水平和基因單核苷酸多態(tài)性與系統(tǒng)性紅斑狼瘡的相關性研究[D];安徽醫(yī)科大學;2013年

本文編號：1745994