本文選題：塊菌　切入點：蛹蟲草　出處：《山東師范大學》2012年碩士論文

【摘要】：塊菌屬于子囊菌門（Ascomycota），盤菌綱（Pezizomycetes），盤菌目（Pezizales），塊菌科（Tuberaceae），是一種藥食兩用真菌，又名松露和豬拱菌，主要分布在西歐的法國、意大利、西班牙等國家；在中國主要分布在四川省和云南省的東南地區(qū)和西北地區(qū)。在國外，有著悠久的食用塊菌的歷史，塊菌也是法國的三大名菜之一。研究表明塊菌具有多種有效活性成分，例如α-雄烷醇的化學結構與人體性激素的化學結構相似，具有調節(jié)女性月經周期等作用；塊菌多糖具有抑制腫瘤細胞生長，調節(jié)機體免疫功能的作用等。目前對塊菌主要進行分類學特征，有效活性成分分離、鑒定等方面的研究，雖然塊菌有著悠久的食用歷史，但對于塊菌保健功效方面的研究卻未見報道。 本文用塊菌子實體粉末的混懸液對小鼠進行負重游泳實驗，實驗結果顯示，實驗組小鼠的負重游泳時間比對照組小鼠的負重游泳時間有明顯的延長，說明塊菌能夠顯著地提高小鼠的耐疲勞能力。 本文用塊菌子實體粉末的混懸液對小鼠進行常壓耐缺氧實驗和亞硝酸鈉急性中毒實驗，結果表明經過塊菌混懸液灌胃處理的小鼠在常壓缺氧和急性中毒缺氧兩種情況下的存活時候均比對照組小鼠存活時間長，由此可見塊菌可以提高機體的耐缺氧能力。 本文用塊菌子實體粉末的混懸液對小鼠進行戊巴比妥鈉誘導睡眠的實驗，實驗數(shù)據(jù)顯示，實驗組小鼠的戊巴比妥鈉誘導睡眠時間與對照組小鼠的戊巴比妥鈉誘導睡眠時間相近，實驗組小鼠的睡眠時間并沒有顯著的延長。實驗結果取平均值數(shù)據(jù)差距可忽略不計，由此得出塊菌對于延長小鼠睡眠時間沒有顯著效果，塊菌沒有鎮(zhèn)靜、催眠等作用�？赏ㄟ^其他實驗方法做進一步的研究證實。 本文用塊菌子實體粉末的混懸液對小鼠進行腸胃運動的研究，通過對實驗組小鼠和對照組小鼠的墨汁推進率的比較可知，較之于對照組，實驗組小鼠小腸內的墨汁推進長度明顯大于對照組小鼠的推進長度，由此可知，塊菌能增強腸胃的活動，幫助消化吸收，促進新陳代謝。 本文還通過對50名原發(fā)性痛經女性進行跟蹤調查，通過統(tǒng)計記錄這50名女性服用塊菌混懸液后的反應得出經前和經期中服用塊菌混懸液可以有效地減輕經期腹痛，并改善身體狀態(tài)。 本文用塊菌子實體粉末的混懸液對小鼠進行造血功能的研究，，通過血液分析儀進行分析，結果顯示實驗組小鼠的紅細胞、血小板、血紅蛋白等指標與對照組相比均有所提高。說明塊菌能夠促進造血干細胞增殖分化，促進小鼠的造血功能。 本文還用蛹蟲草的菌絲體進行上述重復實驗，并將實驗結果與塊菌實驗結果進行對比得出，在戊巴比妥鈉誘導睡眠實驗中蛹蟲草能夠明顯的延長戊巴比妥鈉誘導的小鼠睡眠時間，這與之前有關蛹蟲草具有鎮(zhèn)靜、催眠作用的文獻記載相符，但塊菌在實驗中沒有延長小鼠的睡眠時間，除此以外，其他實驗結果塊菌均明顯優(yōu)于蛹蟲草。
[Abstract]:The truffle belongs to Ascomycota , Petritales , Petritales , and Truaceae . It is a kind of dual - purpose medicine and food fungus , also known as truffle and swine arch , which are mainly distributed in countries such as France , Italy , Spain and other countries in Western Europe ;
In the south - east and northwest of Sichuan and Yunnan Province , China has a long history of edible truffle , and truffle is one of the three major dishes in France . The research shows that truffle has many active ingredients , such as the chemical structure of 偽 - androstanols is similar to the chemical structure of human sex hormone , and has the function of regulating female menstrual cycle .
The truffle polysaccharide has the functions of inhibiting tumor cell growth , regulating immunity of organism , etc . Currently , it is mainly used for taxonomic character , isolation and identification of active ingredients . Although truffle has a long history of eating , the research on the efficacy of truffle has not been reported .
The results showed that the weight swimming time of the mice in the experimental group was significantly prolonged than that in the control group , and the results showed that the weight swimming time of the mice in the experimental group was significantly prolonged than that of the control group , and the results showed that the truffle could obviously improve the fatigue resistance of mice .
The results showed that the survival time of mice under normal pressure hypoxia and acute poisoning was longer than that of the control group . The results showed that truffle could improve the hypoxia tolerance of mice .
The experimental data show that the sleep time of mice in the experimental group is similar to that of the control group , and the experimental data show that the sleep time of mice in the experimental group is similar to that of the control group , and the sleep time of the experimental group is not prolonged . The results show that the truffle has no obvious effect on prolonging the sleep time of the mice , and the truffle has no obvious effect on prolonging the sleep time of the mice , and the truffle has no sedative and hypnotic effects .
Compared with the control group , the propulsive length of the ink in the small intestine of the experimental group was obviously higher than that of the control group , so the truffle could enhance the activity of the intestines and stomach , help the digestion and absorption and promote the metabolism .
Through the follow - up investigation of 50 women with primary dysmenorrhea , the reaction of 50 women taking the truffle suspension after taking the suspension of truffle by statistical recording leads to the effective reduction of menstrual abdominal pain and the improvement of the body state by taking the truffle suspension in the premenstrual period and the menstrual period .
In this paper , the hematopoietic function of mice was studied by using the suspension of the solid powder of truffle . The results showed that the indexes of red blood cells , platelets and hemoglobin in the experimental group were improved compared with the control group . The results showed that the truffle could promote the proliferation and differentiation of hematopoietic stem cells and promote the hematopoietic function of the mice .
The experiments were carried out with mycelia of Cordyceps militaris , and the experimental results were compared with the results of truffle experiment .

【學位授予單位】：山東師范大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R371

【參考文獻】

相關期刊論文 前10條

1 孫維峰,徐偉,王小春,韋嵩,羅曉艷,肖登巖;升降湯對小鼠胃腸蠕動及胃腸激素的影響[J];安徽中醫(yī)學院學報;2002年01期

2 劉潔，楊旭，陳正，梁曼義，李景洛;蠶蛹蟲草鎮(zhèn)靜及性激素樣作用的研究[J];白求恩醫(yī)科大學學報;1994年01期

3 仲偉鑒,張小強,浦躍樸,肖萍;冬蟲夏草與人工蟲草菌絲體無機元素含量的比較[J];環(huán)境與職業(yè)醫(yī)學;2004年04期

4 溫魯;翁梁;朱明偉;劉森琴;;不同林區(qū)蛹蟲草活性成分含量的比較[J];林業(yè)科學;2008年08期

5 尕貝;;蒙醫(yī)治療痛經52例[J];中國民族民間醫(yī)藥;2009年10期

6 劉東澤,陳偉,高新華,代光輝,吳畏;蟲草菌素(3’-脫氧腺苷)研究進展(綜述)[J];上海農業(yè)學報;2004年02期

7 溫魯,尹起范,唐玉玲,夏敏,楊雁玲;蠶蟲草與有關蟲草活性成分檢測比較[J];食品科學;2004年08期

8 陶愷,劉波;中國塊菌的生態(tài)和營養(yǎng)價值[J];山西大學學報(自然科學版);1990年03期

9 鄭忠志,胡斌,畢玉彪,祖靜,孫文閣;葛根素對成年小鼠子宮的影響[J];武警醫(yī)學;2005年03期

10 華永慶;段金廒;朱荃;王秋娟;;縮宮素誘導的小鼠離體痛經模型的實驗方法研究[J];中國藥理學通報;2008年04期

本文編號：1728407