本文選題：腦肉芽腫　切入點：動物模型　出處：《中國病原生物學(xué)雜志》2017年09期

【摘要】：目的通過頸動脈注射血吸蟲蟲卵建立家兔腦肉芽腫模型。方法實驗組家兔10只,行全腦放療(20GY,單次照光)破壞血腦屏障;照光后7d,通過頸動脈注射血吸蟲蟲卵懸浮液1ml建模。陰性對照組10只,全腦放療后7d經(jīng)頸動脈注射生理鹽水1ml�？瞻讓φ战M10只,不進行任何處理。術(shù)后使用抗生素預(yù)防感染,觀察3組動物臨床表現(xiàn)。30d后行MRI檢查。取腦組織做病理切片,觀察腦組織大體標本及病理改變。結(jié)果實驗組2只、陰性對照組1只家兔死于放療后并發(fā)癥。實驗組8只家兔術(shù)后均出現(xiàn)輕度食欲不振,體重下降,1周后癥狀逐漸消失;8只出現(xiàn)呆滯、嗜睡等精神癥狀。陰性對照組家兔術(shù)后均食欲下降,1周后癥狀消失,無異常精神癥狀�？瞻讓φ战M未見異常。MRI檢查實驗組增強T1WI見腦膜線狀強化4例,腦組織內(nèi)見結(jié)節(jié)狀強化2例。對照組未見明顯異常。腦組織大體標本及病理切片檢查實驗組8只家兔均出現(xiàn)血管炎,7只出現(xiàn)腦水腫,6只病理切片見肉芽腫結(jié)節(jié),5只出現(xiàn)腦膜炎。陰性對照組及空白對照組未見腦水腫及肉芽腫結(jié)節(jié)(陰性對照組1只出現(xiàn)血管周圍炎)。結(jié)論經(jīng)頸動脈注射血吸蟲蟲卵建立的家兔腦肉芽腫模型出現(xiàn)相應(yīng)的組織病理變化及臨床表現(xiàn),符合實驗要求。
[Abstract]:Objective through carotid artery injection of schistosome eggs to establish cerebral granuloma model in rabbits. Methods the experimental group of 10 rabbits underwent whole brain radiotherapy (20GY, single light) disruption of the blood-brain barrier; illumination of 7D, through carotid artery injection of Schistosoma japonicum egg suspension 1ml modeling. The negative control group 10, whole brain radiotherapy 7d carotid artery injection of saline 1ml. 10 rats in the blank group, without any treatment. Postoperative use of antibiotics to prevent infection, observe 3 groups of animal clinical manifestations of.30d MRI was performed. The brain tissue was taken for pathological observation of brain tissue specimens and pathological changes. Results in the experimental group, negative control group 2, 1 rabbits died of complication after radiotherapy. The experimental group of 8 rabbits after operation showed mild loss of appetite, weight loss, 1 weeks after the symptoms gradually disappeared; 8 will appear dull, mental symptoms of sleepiness. Negative control group rabbits after operation were decreased appetite, 1 weeks after the symptoms disappear Lost, no abnormal mental symptoms. The blank control group showed no abnormal.MRI examination of the experimental group enhanced T1WI linear meningeal enhancement in 4 cases, brain tissue in nodular enhancement in 2 cases. The control group had no obvious abnormalities. Brain tissue specimens and pathological examination of the experimental group of 8 rabbits showed vasculitis, 7 appeared cerebral edema. 6 pathological sections showed granulomatous nodules, 5 meningitis. The negative control group and blank control group had no brain edema and granuloma nodules (negative control group 1 rats showed perivascular inflammation). Rabbit cerebral granuloma model conclusion through carotid artery injection of schistosome eggs established corresponding histopathologic changes and clinical manifestations, in line with the experimental requirements.

【作者單位】： 江南大學(xué)附屬醫(yī)院;江南大學(xué)公共衛(wèi)生學(xué)院;江蘇省血吸蟲病防治研究所;浙江中醫(yī)藥大學(xué)動物實驗研究中心;浙江大學(xué)附屬第二醫(yī)院放療科;江南大學(xué)醫(yī)學(xué)院;
【基金】：江南大學(xué)公共衛(wèi)生研究中心立項項目(No.1286010242150640)
【分類號】：R-332;R532.21

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