本文選題：精液凝固蛋白　切入點(diǎn)：精子細(xì)胞膜　出處：《昆明醫(yī)學(xué)院》2011年碩士論文

【摘要】：目的：研究人精液凝固蛋白Ⅰ小分子衍生合成肽SgⅠ-52對精子活動的影響并初步探討其可能的機(jī)制。 方法：用Percoll不連續(xù)密度梯度離心法篩選出正常精液中的活動精子,在不去膜以及去膜的處理后,與不同濃度的SgⅠ-52進(jìn)行不同時間的作用,在計算機(jī)輔助精液分析(CASA)下觀察精子活動的變化,用SPSS軟件進(jìn)行統(tǒng)計分析,所得結(jié)果結(jié)合透射電子顯微鏡下未去膜精子及去膜精子結(jié)構(gòu)的特點(diǎn),對SgⅠ小分子衍生合成肽SgⅠ-52對精子活動產(chǎn)生影響的機(jī)制進(jìn)行初步探討。 結(jié)果：去膜精子試驗中,SgⅠ-52的100pg/個精子和200pg/個精子兩種濃度在90min內(nèi)都使精子活動的各項參數(shù)明顯下降(P＜0.05)；不同濃度SgⅠ-52對去膜精子活動影響的差異表現(xiàn)為VCL和BCF參數(shù)的差異(P＜0.05)；在各個濃度組內(nèi),數(shù)據(jù)的變化與作用時間明顯相關(guān)。 未去膜精子試驗中,100pg/個精子濃度的SgⅠ-52在90min內(nèi)使精子的活率下降(P＜0.05),200pg/個精子濃度的SgⅠ-52使活率、A級、B級以及ALH、STR下降(P＜0.05)；不同濃度SgⅠ-52對未去膜精子活動影響的差異表現(xiàn)為VCL、VSL和BCF參數(shù)的差異(P＜0.05)；在各個濃度組內(nèi),數(shù)據(jù)的變化亦與作用時間明顯相關(guān)。 去膜精子和未去膜精子試驗比較,去膜精子組精子活動參數(shù)的改變與濃度的相關(guān)性要小于未去膜精子組,表現(xiàn)為去膜精子試驗中不同濃度間精子活動參數(shù)的變化要小,低濃度的SgⅠ-52就能明顯抑制去膜精子的活動。 結(jié)論：SgⅠ-52對去膜精子和未去膜精子的活動均有明顯的抑制活性,該抑制活性呈劑量/時效相關(guān)性,且SgⅠ-52對去膜精子的抑制效應(yīng)明顯強(qiáng)于對未去膜精子的。由此推測細(xì)胞膜的完整性對于精子的正常活動十分重要,SgⅠ-52可能作用于細(xì)胞膜抑制精子的活動；對于膜被破壞但內(nèi)部結(jié)構(gòu)完整的活動精子,SgⅠ-52可能作用于細(xì)胞內(nèi)抑制精子的活動。
[Abstract]:Objective: To study the effect of human seminal coagulin protein I Sg I -52 on sperm motility and to explore its possible mechanism.
Methods: using Percoll discontinuous density gradient centrifugation were normal in semen sperm, not to go in film and film, with different concentrations of Sg I -52 for different time, analysis in computer aided semen (CASA) changes were observed under the activity of the sperm, analyzed by SPSS the results of software, combined with the characteristics of the transmission electron microscope did not go to the sperm membrane and the sperm membrane structure, mechanism of influence on Sg of small molecule derived synthetic peptides of Sg I -52 on the activity of the sperm was investigated.
Results: the sperm membrane test, Sg I -52 100pg/ 200pg/ two sperm and sperm concentration in 90min the parameters of sperm activity decreased significantly (P < 0.05); the difference between effects of different concentrations of Sg I -52 of demembranated sperm activity showed VCL and BCF parameters (P < 0.05); in each concentration group, was significantly correlated with the changes of time data.
Not to test the sperm membrane, sperm concentration 100pg/ Sg 1 -52 in 90min in the live rate of sperm decreased (P < 0.05), 200pg/ Sg I -52 the sperm concentration, viability, Grade A, B and ALH, STR decreased (P < 0.05); different concentrations of Sg on -52 1 go to the sperm membrane induced differences of VCL between VSL and BCF parameters (P < 0.05); in each concentration group, the change of the data was also correlated with the duration of action.
Compared to the sperm membrane and not to the sperm membrane test, correlation and concentration of demembranated sperm motility parameters of the group is smaller than the demembranated sperm group, changes in the performance of the sperm activity parameters among different concentration to the sperm membrane test in small, low concentration of Sg of -52 can inhibit the sperm membrane activity.
Conclusion: Sg I -52 on to the sperm membrane and not to the sperm membrane activity had inhibitory activity, dose / aging correlation between the inhibitory activity and the inhibitory effect of Sg on the -52 to the sperm membrane was stronger than to go to the sperm membrane integrity. Which indicated that the cell membrane is very important for the normal activities sperm, Sg I -52 may function in the cell membrane to inhibit sperm motility; membrane damage but complete structure of sperm activity, Sg I -52 may play a role in intracellular inhibition of sperm motility.

【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R321.1

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