本文選題：華支睪吸蟲　切入點(diǎn)：DC細(xì)胞　出處：《中國人獸共患病學(xué)報(bào)》2017年06期

【摘要】：目的觀察華支睪吸蟲成蟲抗原(Crude antigen,CA)、排泄/分泌產(chǎn)物(excretory-secretory products,ESPs)對T細(xì)胞的作用。方法體外分離小鼠骨髓細(xì)胞誘導(dǎo)分化為未成熟骨髓樹突狀細(xì)胞(immature DC,iDC);磁珠分選儀分選小鼠脾臟細(xì)胞的初始CD4+T細(xì)胞;流式細(xì)胞術(shù)檢測DC、CD4+T細(xì)胞的純度;抗原刺激DC細(xì)胞,實(shí)驗(yàn)分為PBS陰性對照組,LPS陽性對照組,CA和ESPs刺激組;負(fù)載抗原后的DC細(xì)胞與分選的CD4+T細(xì)胞共培養(yǎng)72h;Real time-PCR檢測T-bet、GATA3mRNA的相對表達(dá)量;ELISA檢測細(xì)胞培養(yǎng)上清中IFN-γ、IL-4細(xì)胞因子的表達(dá)量。結(jié)果與PBS組相比,ESPs刺激組Tbet、GATA3mRNA表達(dá)水平升高(P0.05),而CA刺激組T-bet、GATA3 mRNA表達(dá)水平差異不顯著;與PBS組相比,ESPs刺激組細(xì)胞因子IFN-γ、IL-4的含量均升高(P0.05),CA刺激組僅IFN-γ的分泌增高(P0.05),IL-4無明顯變化。結(jié)論 CA可能誘導(dǎo)宿主產(chǎn)生Th1型免疫應(yīng)答,ESPs可能誘導(dǎo)宿主產(chǎn)生Th1型、Th2型免疫應(yīng)答。
[Abstract]:Objective to observe the effects of the antigens of Clonorchis sinensis (Crude antigenia CAA), excretory-secretory products (ESPs) on T cells.Methods Murine bone marrow cells were isolated and differentiated into immature bone marrow dendritic cells in vitro. The initial CD4 T cells were isolated from mouse spleen cells by magnetic bead sorter, the purity of CD4 T cells was detected by flow cytometry, and DC cells were stimulated by antigen.The experiment was divided into PBS negative control group, LPS-positive control group, CA and ESPs stimulation group, DC cells loaded with antigen were co-cultured with CD4 T cells for 72 h, Real time-PCR was used to detect the relative expression of T-bettgata-GATA3 mRNA and Elisa was used to detect the expression of IFN- 緯 IL-4 cytokines in the supernatant of cell culture.緇撴灉涓嶱BS緇勭浉姣，

本文編號：1708559