本文選題：大豆異黃酮　切入點：子宮內膜異位癥　出處：《延邊大學》2011年碩士論文

【摘要】：目的：探討大豆異黃酮(Soybean Isoflavone, SIF)對子宮內膜異位癥(endometriosis, EMs)大鼠模型中Foxp3及COX-2表達的影響。 方法：將50只Wistar雌性大鼠采用外科手術大鼠自體子宮內膜移植法建立大鼠子宮內膜異位癥模型,隨機分對照組、大豆異黃酮高劑量組、大豆異黃酮低劑量組。造模成功后給藥6周,并做病理學檢查。觀察比較治療前后移植物體積大小,并通過免疫組織化學SABC法檢測大鼠異位內膜組織中Foxp3及COX-2的表達。 結果：1.移植物外觀：對照組與SIF低劑量組移植物體積無明顯差異(P0.05),SIF高劑量組移植物體積明顯縮小(PO.01)； 2.病理學改變：與對照組比較,治療組移植腺體上皮細胞變小上皮細胞層厚度變薄,其中SIF高劑量組效果比SIF低劑量組明顯； 3.與對照組比較,治療組腺體中Foxp3表達率明顯降低(P0.05),且SIF高劑量組中Foxp3的下降程度比SIF低劑量組的更明顯(P0.05)； 4.與對照組比較,治療組腺體中COX-2表達率明顯降低(P0.01)；且SIF高劑量組COX-2表達的下降程度比SIF低劑量組更明顯(P0.05)； 5.異位癥內膜中Foxp3的表達與COX-2的表達呈正相關(r=0.881,P0.01)。 結論：1.高劑量的大豆異黃酮對大鼠子宮內膜異位癥模型中的異位內膜組織具有明顯的抑制作用,其機制可能與大豆異黃酮下調了異位組織中COX-2及Foxp3的表達,從而抑制異位組織中新生血管的生成及提高機體免疫監(jiān)視功能有關。 2.大鼠EMs的異位內膜中COX-2的表達與Foxp3的表達呈正相關,提示血管生成及免疫抑制因素在EMs的發(fā)生機制中起協(xié)同作用。
[Abstract]:Aim: to investigate the effect of soybean isoflavone Isoflavone (siff) on the expression of Foxp3 and COX-2 in endometriosis rat model.Methods: 50 Wistar female rats were randomly divided into control group, high dose daidzein group and low dose daidzein group.The model was successfully treated for 6 weeks, and pathological examination was made.The volume of grafts before and after treatment was observed and the expression of Foxp3 and COX-2 in ectopic endometrium of rats was detected by immunohistochemical SABC method.The result is 1: 1.Graft appearance: there was no significant difference in graft volume between the control group and the low dose SIF group.2.Pathological changes: compared with the control group, the thickness of epithelial cell layer of transplanted glandular epithelial cells in the treatment group was thinner, and the effect of SIF in high dose group was more obvious than that in SIF low dose group.3.Compared with the control group, the expression rate of Foxp3 in the glands of the treatment group was significantly lower than that of the control group, and the decrease of Foxp3 in the high dose group of SIF was more significant than that in the low dose group of SIF.4.Compared with the control group, the expression rate of COX-2 in the glands of the treatment group was significantly lower than that of the control group, and the decrease of COX-2 expression in the high dose group of SIF was more significant than that in the low dose group of SIF.5.There was a positive correlation between the expression of Foxp3 and COX-2 in endometrium.Conclusion 1.High dose soybean isoflavones could inhibit the expression of COX-2 and Foxp3 in the ectopic endometrium of rats, and the mechanism might be related to the down-regulation of the expression of COX-2 and Foxp3 in the ectopic tissues by soybean isoflavones.It is related to the inhibition of angiogenesis in ectopic tissues and the enhancement of immune surveillance function.2.The expression of COX-2 in ectopic endometrium of rat EMs was positively correlated with the expression of Foxp3, suggesting that angiogenesis and immunosuppressive factors play a synergistic role in the pathogenesis of EMs.
【學位授予單位】：延邊大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R711.71;R-332

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