本文選題：鹽酸氯胺酮　切入點(diǎn)：兔血清白蛋白　出處：《云南農(nóng)業(yè)大學(xué)學(xué)報(自然科學(xué))》2016年06期

【摘要】：基于鹽酸氯胺酮是一種化學(xué)反應(yīng)性質(zhì)活潑的小分子,利用半抗原—載體效應(yīng)的免疫學(xué)原理,以三氯化磷(PCl3)為橋梁,將鹽酸氯胺酮分子共價連接到兔血清白蛋白(RSA)分子,隨后采用十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)分析偶聯(lián)物,并用該偶聯(lián)物免疫BALB/c小鼠,最后采用斑點(diǎn)酶聯(lián)免疫吸附試驗(yàn)(dot-ELISA)檢測氯胺酮抗體應(yīng)答水平。蛋白電泳分析顯示已成功制備出分子量約為78.8 ku、氯胺酮與RSA的分子偶聯(lián)比為39的完全抗原,能誘導(dǎo)實(shí)驗(yàn)小鼠產(chǎn)生效價為1∶1 280的抗氯胺酮抗體。本試驗(yàn)不僅建立了檢測氯胺酮的血清學(xué)方法,還為開發(fā)氯胺酮戒毒疫苗做了有益的積累。
[Abstract]:Based on the fact that ketamine hydrochloride is a small molecule with active chemical reaction properties, using the immunological principle of hapten carrier effect, the ketamine hydrochloride molecule is covalently linked to rabbit serum albumin (RSA) molecule by using phosphorous trichloride (PCL _ 3) as a bridge.BALB/c mice were immunized with sodium dodecyl sulfate and polyacrylamide gel electrophoresis (SDS-PAGE). Finally, dot-ELISAs were used to detect the level of ketamine antibody response.Electrophoretic analysis showed that the complete antigen with molecular weight of 78.8 and molecular coupling ratio of Ketamine to RSA of 39 was successfully prepared, which could induce the production of anti-ketamine antibody with a titer of 1:1 280 in experimental mice.This study not only established a serological method for the detection of ketamine, but also made a beneficial accumulation for the development of ketamine abstinence vaccine.
【作者單位】： 云南農(nóng)業(yè)大學(xué)動物科學(xué)技術(shù)學(xué)院;昆明科靈生物科技有限公司;
【基金】：云南農(nóng)業(yè)大學(xué)第七屆學(xué)生科技創(chuàng)新創(chuàng)業(yè)行動基金項目(2013KFX001)
【分類號】：R392-33
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本文編號：1701694