本文選題：肝郁　切入點(diǎn)：精瘀　出處：《成都中醫(yī)藥大學(xué)》2011年碩士論文

【摘要】：目的 基于中醫(yī)肝疏泄功能具有疏通暢達(dá)氣機(jī),調(diào)暢情志,協(xié)調(diào)沖任二脈,促進(jìn)生殖之精排泄的理論,及前期研究組對敗精(精瘀)理論、實(shí)驗(yàn)研究的基礎(chǔ)上,本研究在使用慢性束縛應(yīng)激法造成情志抑郁——肝氣郁結(jié)的前提下,通過電動(dòng)刺激棒建立雄性大鼠肝郁致精瘀的動(dòng)物模型,以探討情志——肝郁——精瘀的相關(guān)性,為探討肝郁致精瘀的機(jī)理、肝郁精瘀的致病性、肝郁精瘀所致各種疾病的治療提供依據(jù)。 方法 將75只雄性SD大鼠隨機(jī)分為四組,即第一組為對照組30只,第二組為肝郁模型組15只,第三組為肝郁藥物組15只,第四組為肝郁精瘀組15只。采用慢性束縛應(yīng)激方法,將大鼠置于特制的束縛筒內(nèi)4周建立肝郁模型的基礎(chǔ)上,給第四組大鼠使用自制電動(dòng)刺激棒刺激10日建立肝郁精瘀模型,并通過觀察生殖性腺組織相關(guān)指標(biāo)的改變以確立肝郁致精瘀模型的建立。 結(jié)果 研究發(fā)現(xiàn)：肝郁模型組、肝郁精瘀組與對照組大鼠相比較,行為多表現(xiàn)為倦怠少動(dòng)、叫聲尖細(xì)、反應(yīng)遲緩、飲食減少、毛色晦暗發(fā)黃、大便顆粒松散甚至呈水樣便等。對照組與肝郁模型組、肝郁藥物組尾懸掛實(shí)驗(yàn)掙扎次數(shù)三組之間存在顯著差異(p0.01)。大鼠血漿ACTH檢測對照組與肝郁模型組、肝郁藥物組三組之間存在顯著差異(p0.05)。血漿CORT檢測正常對照組與肝郁模型組、肝郁藥物組三組之間存在著顯著的差異(p0.05)。分別從藥物治療前后行為學(xué)表現(xiàn)、神經(jīng)內(nèi)分泌系統(tǒng)指標(biāo)差異兩個(gè)方面驗(yàn)證慢性束縛應(yīng)激建立肝郁證大鼠模型的可靠性。對大鼠生殖腺組織的ACP檢測(P0.05),及α-Glu檢測(P0.05),肝郁精瘀組和正常對照組之間無明顯差異。但造模過程中,使用電動(dòng)刺激棒刺激后,大部分大鼠在靜息狀態(tài)下陰莖口內(nèi)可見白色分泌物,符合中醫(yī)白濁病證臨床表現(xiàn)中滴白癥狀,在一定程度上表現(xiàn)出了精瘀的存在。 結(jié)論 本次研究采用慢性應(yīng)激束縛筒制動(dòng)及電動(dòng)刺激棒刺激的方法,肝郁精瘀組大鼠表現(xiàn)出在刺激期間陰莖口有滴白現(xiàn)象,在一定程度上表現(xiàn)出了精瘀的存在,說明了肝郁與精瘀具有相關(guān)性,為進(jìn)一步深入研究做了有意義的嘗試。
[Abstract]:Purpose. Based on the theory that the function of traditional Chinese medicine (TCM) to soothe the liver and relieve the discharge of qi, regulate the emotion, coordinate the two veins, and promote the excretion of the essence of reproduction, and on the basis of the experimental study on the theory of sterility (blood stasis) in the early stage of the study group, In this study, on the premise that chronic restraint stress was used to cause depression of emotion and depression of liver-qi, the animal model of blood stasis induced by liver depression in male rats was established by electric stimulation rod, in order to explore the correlation between emotion, liver-depression and blood stasis. In order to study the mechanism of liver depression and blood stasis, the pathogenicity of liver stagnation and the treatment of various diseases caused by liver stagnation and essence stasis. Method. Seventy-five male SD rats were randomly divided into four groups: control group (n = 30), model group (n = 15), drug group (n = 15) and group IV (n = 15). On the basis of the model of liver-depression induced by Zhou Jianli in a special restraint tube, the rats in the fourth group were stimulated with self-made electric stimulator for 10 days to establish the model of liver depression and blood stasis. By observing the changes of related indexes of reproductive gonad tissue, the model of liver depression and essence blood stasis was established. Results. The results showed that compared with the control group, the rats in the liver depression model group and the liver depression essence stasis group were characterized by burnout, thin call, slow response, reduced diet, dark and yellowish coat color. There were significant differences in the number of experimental struggles between the control group and the liver depression model group and the liver depression drug group. There was a significant difference among the three groups (p 0.01). The plasma ACTH was detected in the control group and the liver depression model group. There was a significant difference among the three groups in liver depression drug group (P 0.05). There was significant difference in plasma CORT between normal control group and liver depression model group, and there was significant difference between three groups (P 0.05). The reliability of the rat model of liver depression induced by chronic restraint stress was verified in two aspects of the difference of neuroendocrine system indexes. ACP and 偽 -Glu in gonad tissue of rats were detected by P0.05A and 偽 -Glu, respectively, and there was no clear relationship between the group with stagnation of liver and the control group. But in the process of modeling, After stimulation with electric stimulation rod, white secretion could be seen in the penile mouth of most rats under resting state, which was in line with the symptoms of white turbid disease syndrome in TCM, and showed the presence of essence stasis to some extent. Conclusion. In this study, by using the methods of chronic stress restraint tube braking and electric stimulation rod stimulation, the rats in the group of liver depression and Jingyu showed the phenomenon of dropping white penis during the period of stimulation, and to some extent showed the presence of fine blood stasis. The relationship between liver depression and blood stasis was explained, and a meaningful attempt was made for further study.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 喬明琦,張惠云,陳雨振,韓秀珍,姚澤賢,劉長華,宋承木;肝郁證動(dòng)物模型研究的理論思考[J];中國醫(yī)藥學(xué)報(bào);1997年05期

2 王愛成,王玉來,李柏,陳錦峰,尹嶺,尹大一,姚樹林,單保慈;肝氣郁結(jié)證相關(guān)腦區(qū)的正電子發(fā)射斷層成像[J];中華中醫(yī)藥雜志;2005年08期

3 覃菁,徐建陽;中醫(yī)肝郁證的動(dòng)物模型研究概況[J];廣西中醫(yī)藥;2003年06期

4 楊冬花,李家邦,鄭愛華,蔣榮鑫;肝氣郁結(jié)證模型大鼠Th1/Th2細(xì)胞因子變化及柴胡疏肝散的干預(yù)作用[J];湖南中醫(yī)學(xué)院學(xué)報(bào);2003年05期

5 毛海燕;肝郁證大鼠肝線粒體膜流動(dòng)性 血漿胃動(dòng)素及血清胃泌素的變化及意義[J];福建中醫(yī)藥;2003年03期

6 毛海燕,葉林,葉向榮;肝郁證大鼠中樞神經(jīng)遞質(zhì)變化的觀察[J];福建中醫(yī)藥;2002年02期

7 王伯軍,丁勇,馬微波,季林根;胃腸道疾病與情緒障礙的相關(guān)因素調(diào)查分析[J];臨床醫(yī)學(xué);2005年06期

8 王勁松;王曉虎;徐福松;曾慶琪;;對中醫(yī)男科理論研究新說之探析[J];遼寧中醫(yī)雜志;2006年01期

9 常德貴,王崢屹,祝彼得,王久源,張蜀武,胡志萍;疏肝理氣活血法對肝郁氣滯大鼠陰莖海綿體PDE5活性影響的實(shí)驗(yàn)研究[J];中華男科學(xué);2004年01期

10 金光亮,南睿,郭霞珍;慢性應(yīng)激肝郁證大鼠模型的建立[J];北京中醫(yī)藥大學(xué)學(xué)報(bào);2003年02期

相關(guān)博士學(xué)位論文 前2條

1 王海軍;經(jīng)前期綜合征肝氣逆證辨證規(guī)范及療效評價(jià)量表研究[D];山東中醫(yī)藥大學(xué);2007年

2 鐘小蘭;肝郁證模型大鼠蛋白質(zhì)組差異表達(dá)研究[D];第一軍醫(yī)大學(xué);2006年

，

本文編號：1700439