本文選題：顱腦損傷　切入點(diǎn)：柴胡細(xì)辛湯　出處：《中醫(yī)雜志》2017年08期

【摘要】：目的觀察柴胡細(xì)辛湯加減對(duì)顱腦損傷模型大鼠認(rèn)知功能和神經(jīng)功能缺損的作用及其可能機(jī)制。方法將89只SD大鼠隨機(jī)分為假手術(shù)組16只,模型組、陽性對(duì)照組和中藥低劑量組各18只,中藥高劑量組19只,除假手術(shù)組外其余各組建立控制性皮層沖擊損傷模型。造模次日陽性對(duì)照組予安理申片0.45 g/(kg·d),中藥低、高劑量組分別給予柴胡細(xì)辛湯加減方6.5、13 g生藥/(kg·d),假手術(shù)組、模型組予等體積雙蒸水,各組灌胃4周。比較各組大鼠認(rèn)知功能(水迷宮測(cè)試)、神經(jīng)功能缺損評(píng)分(m NSS)、腦組織病理與海馬CA1區(qū)超微結(jié)構(gòu),并檢測(cè)CA1區(qū)腦源性神經(jīng)生長因子(BDNF)、突觸素1(SYN1)蛋白表達(dá)。結(jié)果各組在造模后0、3、7天時(shí)m NSS評(píng)分較假手術(shù)組均明顯升高(P0.01),中藥高劑量組在第7、28天時(shí)m NSS評(píng)分較模型組降低,中藥低劑量組在28天時(shí)亦低于模型組(P0.05或P0.01)。水迷宮測(cè)試中,各給藥組逃避潛伏期時(shí)間較模型組減少(P0.05或P0.01)。各給藥組損傷海馬區(qū)病理形態(tài)學(xué)顯示優(yōu)于模型組,中藥高、低劑量組能夠改善海馬CA1區(qū)受損的神經(jīng)元、線粒體和突觸的超微結(jié)構(gòu)。中藥高劑量組BDNF表達(dá)明顯高于模型組和陽性對(duì)照組,中藥高劑量組SYN1表達(dá)高于模型組(P0.01)。結(jié)論柴胡細(xì)辛湯加減能夠改善顱腦損傷模型大鼠認(rèn)知功能及其神經(jīng)功能缺損,其機(jī)制可能是提高海馬CA1區(qū)BDNF、SYN1的表達(dá)以促進(jìn)損傷的神經(jīng)元修復(fù)與突觸重塑,改善海馬CA1區(qū)超微結(jié)構(gòu)。
[Abstract]:Objective to observe the effect and possible mechanism of Radix Bupleurum Asarum decoction on cognitive and neurological deficits in rats with craniocerebral injury. Methods Eighty-nine SD rats were randomly divided into sham operation group (n = 16) and model group (n = 16). There were 18 rats in the positive control group and 18 in the low dose group and 19 in the high dose group. The controlled cortical impact injury model was established in the other groups except the sham-operation group. The high-dose group were given 6.5g of crude drug / kg dai, sham-operated group and model group were treated with the same volume of double-steamed water, respectively, in the subtractive prescription of Radix Bupleurum Asarum decoction (6.5g / kg dL). The cognitive function (water maze test, neurological deficit score), brain pathology and ultrastructure of hippocampal CA1 were compared in each group for 4 weeks. The expression of brain-derived nerve growth factor (BDNF) and synaptophysin 1 (SYN 1) protein in CA1 area was detected. Results the m NSS score in each group was significantly higher than that in sham operation group at 7 days after model making, and the m NSS score in high dose group was lower than that in model group at day 728. In the water maze test, the escape latency time of each administration group was lower than that of the model group (P 0.05 or P 0.01). The pathomorphology of the injured hippocampus in each administration group was better than that in the model group, and the traditional Chinese medicine was higher than that in the model group. The ultrastructure of neurons, mitochondria and synapses in the injured neurons, mitochondria and synapses in the CA1 area of hippocampus was improved in the low dose group. The expression of BDNF in the high dose group was significantly higher than that in the model group and the positive control group. Conclusion Radix Bupleurum Asarum decoction can improve the cognitive function and neural function defect in the model rats with craniocerebral injury, and the expression of SYN1 in the high dose group is higher than that in the model group. The mechanism may be to increase the expression of BDNF CA1 SYN1 in hippocampus to promote the repair of injured neurons and synaptic remodeling, and to improve the ultrastructure of hippocampal CA1.
【作者單位】： 上海中醫(yī)藥大學(xué)附屬曙光醫(yī)院;上海市閘北區(qū)中心醫(yī)院;
【基金】：教育部國家重點(diǎn)學(xué)科(100508) 上海市中醫(yī)藥領(lǐng)軍人才建設(shè)項(xiàng)目(滬衛(wèi)中醫(yī)[2012]63號(hào)) 上海領(lǐng)軍人才“地方隊(duì)”培養(yǎng)計(jì)劃(滬人社專領(lǐng)[2010]081號(hào)) 上海市進(jìn)一步加快中醫(yī)藥事業(yè)發(fā)展三年行動(dòng)計(jì)劃(2014年-2016)項(xiàng)目(ZY3-JSFC-1-1025,ZY3-CCCX-1-1003) 上海市“杏林新星”計(jì)劃(ZYSNXD011-RC-XLXX-20130050) 上海市衛(wèi)生局中醫(yī)藥科研基金項(xiàng)目(2012QL002A) 上海市閘北區(qū)衛(wèi)生科研課題(2012ZY01)
【分類號(hào)】：R285.5;R-332

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