本文選題：podoplanin　切入點(diǎn)：CLEC-2　出處：《蘇州大學(xué)》2012年碩士論文

【摘要】：目的：探討跨膜糖蛋白podoplanin在人早孕期母胎界面的表達(dá)及其對(duì)蛻膜基質(zhì)細(xì)胞增殖及侵襲等生物學(xué)功能的影響。 方法：收集正常早孕期蛻膜及絨毛組織，應(yīng)用本實(shí)驗(yàn)室建立的胰酶消化、密度梯度離心法分離和培養(yǎng)蛻膜基質(zhì)細(xì)胞(decidual stromal cells，DSCs)和滋養(yǎng)細(xì)胞（trophoblasts，，Tros）。采用免疫組織化學(xué)、免疫細(xì)胞化學(xué)以及流式細(xì)胞術(shù)分別檢測(cè)podoplanin和及其受體C型凝集素樣受體-2（C-type lectin-like receptor-2，CLEC-2）的表達(dá)水平和特點(diǎn)；并以siRNA干擾技術(shù)沉默蛻膜基質(zhì)細(xì)胞podoplanin的表達(dá)，然后采用MTT比色法檢測(cè)其活力、BrdU細(xì)胞增殖ELISA法檢測(cè)其增殖能力、以流式細(xì)胞術(shù)分析其凋亡情況以及Transwell法分析podoplanin沉默后細(xì)胞侵襲能力的改變。 結(jié)果：正常早孕蛻膜基質(zhì)細(xì)胞和滋養(yǎng)細(xì)胞均表達(dá)podoplanin和CLEC-2，podoplanin在DSCs的表達(dá)水平顯著高于Tros者，而CLEC-2在Tros和DSCs中表達(dá)水平均較低；采用siRNA干擾podoplanin在DSCs的表達(dá)后，在顯著降低細(xì)胞活力并抑制細(xì)胞增殖和侵襲能力的同時(shí)，促進(jìn)了該細(xì)胞的凋亡。 結(jié)論：人早孕期母-胎界面均表達(dá)podoplanin及其受體CLEC-2，且podoplanin在蛻膜基質(zhì)細(xì)胞的表達(dá)水平較高，提示podoplanin可能通過(guò)與內(nèi)源性受體CLEC-2的相互作用參與調(diào)節(jié)DSCs的生物學(xué)功能并維持正常妊娠。Podoplanin可能在促進(jìn)蛻膜基質(zhì)細(xì)胞的增殖和侵襲、抑制其凋亡的信號(hào)通道中發(fā)揮著中介作用，這種作用有利于維持細(xì)胞自身的正常生物學(xué)功能和調(diào)節(jié)滋養(yǎng)細(xì)胞侵襲過(guò)程，從而最終有利于胎盤(pán)的形成和正常妊娠的維持。
[Abstract]:Objective: To investigate the expression of transmembrane glycoprotein Podoplanin in human fetal maternal interface of decidual stromal cell proliferation and invasion of biological function influence.
Methods: collect decidua and villi of early pregnancy, using trypsin digestion established in our laboratory, isolation and culture of decidual stromal cells by density gradient centrifugation (decidual stromal, cells, DSCs) and trophoblast cells (trophoblasts, Tros). By immunohistochemistry, immunocytochemistry and flow cytometry were used to detect Podoplanin and its receptor C type lectin like receptor -2 (C-type lectin-like receptor-2, CLEC-2) expression levels and characteristics; and the siRNA interference technology to silence Podoplanin expression of decidual stromal cells, then MTT assay was used to test the activity, detect the proliferation ability of BrdU cell proliferation by ELISA method, analysis of Podoplanin silencing cells invasion cell apoptosis Transwell method and flow cytometry.
Results: Podoplanin and CLEC-2 were expressed in normal decidual stromal cells and trophoblast cells, Podoplanin expression level in DSCs was significantly higher than that of Tros, but the level of CLEC-2 was lower in Tros and DSCs expression by siRNA interference in Podoplanin; the expression of DSCs, a significant reduction in cell viability and inhibit cell proliferation and invasion ability at the same time the cell apoptosis, promote.
Conclusion: Podoplanin and its receptor CLEC-2 were expressed in human first trimester maternal fetal interface, and Podoplanin in decidual stromal cells expressed higher levels, suggesting that Podoplanin may interact with endogenous CLEC-2 receptor involved in the biological function of the regulation of DSCs and.Podoplanin in normal pregnancy may promote the proliferation and invasion of decidual stromal cells, plays a mediating role the signal channel inhibits cell apoptosis, this effect is conducive to the normal biological function of keeping cell and regulation of trophoblast invasion process, which is conducive to the maintenance of the placenta formation and normal pregnancy.

【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 潘衛(wèi),王應(yīng)雄,黎剛,翁亞光,劉孝云;小鼠胚泡黏附時(shí)子宮內(nèi)膜Galectin-1的變化[J];生殖與避孕;2004年03期

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