本文選題：半乳糖凝集素-　切入點：子宮內(nèi)膜異位癥　出處：《現(xiàn)代婦產(chǎn)科進展》2017年05期

【摘要】：目的:研究半乳糖凝集素-1(gal-1)在大鼠子宮內(nèi)膜異位癥(EMs)模型異位及在位組織中的表達。方法:選取12只性成熟雌性未孕Wistar大鼠,取一側(cè)宮角子宮內(nèi)膜,采用自體移植法建立EMs大鼠模型。術(shù)后28天再次開腹,將建模成功的大鼠作為自身對照,取其異位病灶與在位子宮內(nèi)膜,蘇木精-伊紅(HE)染色鑒定組織學(xué)特征。免疫組織化學(xué)(IHC)法、實時熒光定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(qRT-PCR)及蛋白免疫印跡(Western blot)法檢測異位病灶與在位內(nèi)膜中g(shù)al-1表達。結(jié)果:肉眼觀異位病灶呈橢圓形囊泡狀,內(nèi)含清亮或咖啡色液體,表面及周圍有新生血管。組織學(xué)證實為異位內(nèi)膜。造模成功率為83.33%。IHC顯示,gal-1表達于異位及在位子宮內(nèi)膜基質(zhì);QRT-PCR定量分析表明,異位內(nèi)膜gal-1 mRNA表達量高于在位內(nèi)膜(P0.05);Western blot結(jié)果示,異位內(nèi)膜gal-1蛋白水平升高(P0.05)。結(jié)論:大鼠EMs異位內(nèi)膜中g(shù)al-1表達高于在位內(nèi)膜,為該靶點參與EMs的機制及治療的研究打下基礎(chǔ)。
[Abstract]:Objective: to study the expression of galactose agglutinin (galactose lectin) in ectopic and eutopic tissues of rats with endometriosis. The EMs rat model was established by autologous transplantation. After 28 days of operation, the successful model rats were used as self-control, and the ectopic focus and eutopic endometrium were taken out. Histopathological characteristics of hematoxylin-eosin (HEH) staining. The expression of gal-1 in ectopic lesions and eutopic endometrium was detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot. There were neovascularization on and around the surface. Histologically confirmed ectopic endometrium. The successful rate of modeling was 83.33%.IHC and QRT-PCR quantitative analysis showed that the expression of gal-1 mRNA in ectopic and eutopic endometrial stroma was higher than that in eutopic endometrium (P 0.05), and the results of Western blot showed that the expression of gal-1 in ectopic endometrium was higher than that in eutopic endometrium. Conclusion: the expression of gal-1 in ectopic endometrium of EMs is higher than that in eutopic endometrium, which provides a basis for the study of the mechanism and therapy of EMs.
【作者單位】： 南方醫(yī)科大學(xué)附屬深圳市婦幼保健院;
【基金】：深圳市科創(chuàng)委資助項目(No:JCYJ20150402090412995) 深圳市衛(wèi)計委項目(No:201501034)
【分類號】：R-332;R711.71
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本文編號：1678380