本文選題：卵泡抑素　切入點(diǎn)：激活素　出處：《遼寧醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的 本實(shí)驗(yàn)觀察了Follistatin、Activin A與BMP-4在大鼠大腦皮質(zhì)、紋狀體、海馬和嗅球發(fā)育過程中的表達(dá)變化規(guī)律,籍以探討Follistatin、Activin A與BMP-4在神經(jīng)系統(tǒng)發(fā)育過程中的表達(dá)特點(diǎn)和調(diào)控機(jī)制。 方法 隨機(jī)取同期合籠后查有陰道栓的SD入鼠30只,隨機(jī)分成6組,每組5只。分別為E8.5天組、E13天組、E18天組、P3d(出生后3天)組、P7d組、P30d組。取E8.5組、E13組、E18組孕鼠(各5只)。每只孕鼠取5只胎鼠,取出胎腦固定。其他出生后P3d組、P7d組、P30d組每組隨機(jī)取5只大鼠灌注固定,切取包含皮質(zhì)、紋狀體、海馬、嗅球組織的腦片,每個(gè)部位切8張切片,用于免疫組化FS、Act、BMP-4染色。分別取E8.5、E13、E18、生后P3、1周(記作P3d、PIW)和生后1月(記作P1M)共6組,每組取大鼠5只,分別在冰臺上分離提取大腦皮層、紋狀體、海馬和嗅球總RNA。應(yīng)用免疫組織化學(xué)ABC法和RT-PCR法(半定量)分析Follistatin、Activin A與BMP-4在出生前后大鼠大腦皮質(zhì)、紋狀體、海馬、嗅球表達(dá)水平的變化。 結(jié)果 進(jìn)行免疫組織化學(xué)染色及光鏡觀察Follistatin與Activin A在大鼠腦內(nèi)廣泛分布,二者在胚胎期E8.5表達(dá)最高,并以胚胎期E13表達(dá)強(qiáng)度開始降低,在出生后P1M降至最低,同一發(fā)育階段各腦區(qū)表達(dá)無明顯差異。在大鼠不同發(fā)育階段的相應(yīng)腦區(qū)BMP-4亦廣泛表達(dá),但從胚胎期至新生期P1W持續(xù)低表達(dá),尤以海馬表達(dá)極弱,在生后P1M在不同腦區(qū)呈高表達(dá),各發(fā)育階段以大腦皮質(zhì)和紋狀體表達(dá)略強(qiáng)。RT-PCR結(jié)果顯示：Follistatin mRNA與Activin A mRNA二者在胚胎期E8.5表達(dá)最強(qiáng),胚胎期E13表達(dá)水平開始降低,在E18至生后P1W呈下降趨勢,P1M表達(dá)降至最低。并在大鼠同一發(fā)育年齡各腦區(qū)表達(dá)無明顯差異。BMP-4mRNA在胚胎期至新生期P3呈較弱表達(dá),在生后P1W表達(dá)開始升高,P1M達(dá)到峰值,在大鼠各發(fā)育階段以大腦皮質(zhì)和紋狀體表達(dá)略強(qiáng)。 結(jié)論 Follistatin、Activin A與BMP-4在大鼠不同發(fā)育年齡各腦區(qū)具有波動(dòng)性表達(dá),表達(dá)水平與發(fā)育年齡密切相關(guān)。Follistatin、Activin A、BMP-4與大腦皮質(zhì)、紋狀體、海馬、嗅球的發(fā)育密切相關(guān)。
[Abstract]:Purpose. The expression of Follistatin A and BMP-4 during the development of cerebral cortex, striatum, hippocampus and olfactory bulb in rats was observed in order to investigate the expression characteristics and regulation mechanism of FollistatinActivin A and BMP-4 during the development of nervous system. Method. Thirty SD rats with vaginal suppository were randomly divided into 6 groups. Five rats in each group were divided into E8.5-day group, E13-day group, E18-day group, P3-day group (3 days after birth), P7-day group, P30d group, E8.5 group, E13 group, E18 group (5 rats each). Other postnatal P3 d group, P7 d group, P30 d group, 5 rats in each group were randomly selected for perfusion fixation, and the brain slices containing cortex, striatum, hippocampus and olfactory bulb were cut into 8 sections. Six groups of E8.5E13E18 were used for immunohistochemical staining. The rats were divided into 6 groups: one week after birth (recorded as P3dPIW) and one month after birth (recorded as P1M). Five rats in each group were isolated and extracted from the cerebral cortex and striatum on the ice platform, respectively. The expression of Follistatin A and BMP-4 in cerebral cortex, striatum, hippocampus and olfactory bulb of rats before and after birth were analyzed by immunohistochemical ABC method and RT-PCR method (semi-quantitative). Results. Immunohistochemical staining and light microscopy were used to observe the wide distribution of Follistatin and Activin A in the brain of rats. The expression of E8. 5 was the highest in the embryonic phase, and the expression intensity of E13 began to decrease in the embryonic stage, and the P1m decreased to the lowest after birth. There was no significant difference in the expression of P1W in different brain regions of rats at the same developmental stage, but the expression of P1W continued to be low from embryonic stage to neonatal stage, especially in hippocampus, and the expression of P1M was high in different brain regions after birth. The results of RT-PCR showed that the expression of mRNA and Activin A mRNA were the strongest in embryonic E8.5, and the expression level of E13 in embryonic phase began to decrease. From E18 to postnatal, the expression of P1M decreased to the lowest. There was no significant difference in the expression of BMP-4 mRNA in different brain regions of the rats at the same developmental age. The expression of BMP-4 mRNA was weakly expressed from embryonic stage to newborn stage, and the expression of P1W began to increase after birth, and the expression of P1M reached its peak. The expression of cerebral cortex and striatum was slightly stronger in all developmental stages of rats. Conclusion. The expression level of FollistatinA and BMP-4 in different brain regions of rats at different developmental ages was closely related to the development of cerebral cortex, striatum, hippocampus and olfactory bulb, and the expression level was closely related to the development of the brain cortex, striatum, hippocampus and olfactory bulb, and the level of expression was closely related to the development of the cerebral cortex, striatum, hippocampus and olfactory bulb.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R338

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