本文選題：沒食子提取物　切入點：IgA腎病　出處：《中國藥理學與毒理學雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的研究沒食子提取物(TGE)對Ig A腎病(Ig AN)模型大鼠血清、尿液及腎組織Ig A表達的影響。方法 50只健康雄性Sprague-Dawley大鼠隨機分為正常對照組、Ig AN模型組、TGE 75,150和300 mg·kg~(-1)治療組,每組10只。除正常對照組外,其余各組采用牛血清白蛋白(BSA)+脂多糖(LPS)+四氯化碳(CCl_4)聯合造模,持續(xù)12周。從第13周開始,ig給予TGE,正常對照組及Ig AN模型組給予等量生理鹽水,持續(xù)4周。第12周末及第16周末用BCA法測定24 h尿蛋白;第16周末,酶聯免疫吸附法(ELISA)測定血清及尿液Ig A水平,全自動生化分析儀檢測血清肌酐(SCr)和尿素氮(BUN)水平,按牛津分型對腎組織病理進行評分,免疫熒光法測定腎組織Ig A免疫復合物沉積。結果與正常對照組相比,Ig AN造模大鼠在12周末24 h尿蛋白升高(P0.05);在16周末,Ig AN模型組大鼠24 h尿蛋白、血清和尿液Ig A水平、血清SCr和BUN水平、腎組織牛津病理評分和Ig A沉積均較正常對照組升高(P0.05);與Ig AN模型組相比,TGE各給藥組大鼠24 h尿蛋白、血清和尿液Ig A水平以及血清SCr水平降低(P0.05),TGE 150和300 mg·kg~(-1)組血清BUN水平和腎組織Ig A含量下降(P0.05),僅TGE 300 mg·kg~(-1)組牛津病理評分中有明顯減少。結論 TGE可減少Ig AN模型大鼠血清、尿液及腎組織Ig A水平,對Ig AN具有一定程度的治療作用。
[Abstract]:Objective to study the effect of TGE on the expression of IgA in serum, urine and renal tissue of rats with IgA nephropathy. Methods Fifty healthy male Sprague-Dawley rats were randomly divided into two groups: normal control group (Ig an model group) treated with TGE 75,150 mg 路kg ~ (-1) and control group (300 mg 路kg ~ (-1)). 10 rats in each group were treated with BSA (BSA) and CCl4 (CCl4) in each group for 12 weeks. TGEI was given orally at the 13th week, and the normal control group and the IgAN model group were given the same amount of normal saline, and the control group and the IgAN model group were given the same amount of normal saline, and the control group and the IgAN model group were given the same amount of normal saline. Serum and urine levels of IgA were determined by Elisa at the end of week 16, serum creatinine (creatinine) and bun (bun) were measured by automatic biochemical analyzer. According to the Oxford classification, the renal tissue pathology was graded, Results compared with the normal control group, the urinary protein of IgAN model rats increased P0.05A at the end of 12 weeks and the levels of urinary protein, serum and urine IgA at the end of 16 weeks in the IgAN model group. The levels of serum SCr and BUN, Oxford pathological score and IgA deposition in renal tissue were higher than those in normal control group (P 0.05), and 24 h urine protein of rats in Ig an model group were higher than those in control group. Serum and urine IgA levels and serum SCr levels decreased in P0.05TGE150 and 300mg 路kg-1) groups. The levels of serum BUN and renal tissue IgA decreased P0.05a, only TGE 300mg 路kg-1) group showed a significant decrease in Oxford pathological score. Conclusion TGE can reduce the serum level of IgAN model rats. The level of IgA in urine and renal tissue has some therapeutic effect on IgAN.
【作者單位】： 新疆醫(yī)科大學第一附屬醫(yī)院腎病科;山東省淄博市中心醫(yī)院泌尿內科;新疆醫(yī)科大學附屬中醫(yī)醫(yī)院腎病科;
【基金】：國家自然科學基金(81660127)~~
【分類號】：R29;R-332

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