本文選題：骨髓間充質(zhì)干細(xì)胞　切入點(diǎn)：種子細(xì)胞　出處：《重慶醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：背景間充質(zhì)干細(xì)胞是具有多向分化潛能的非造血干細(xì)胞,已廣泛應(yīng)用于干細(xì)胞移植、組織工程和器官移植免疫治療等方面,特別是成為構(gòu)建組織工程血管中重要的種子細(xì)胞[1]，但其具體機(jī)制不完全清楚。 目的探討在氧濃度改變對大鼠骨髓間充質(zhì)干細(xì)胞粘附能力的影響。 方法采用SD大鼠20只，年齡6-8周、雌雄不限、體重100g-120g，獲取其股骨和脛骨骨髓細(xì)胞懸液，貼壁法分離骨髓間充質(zhì)干細(xì)胞。流式細(xì)胞鑒定實驗細(xì)胞CD54、 CD29、CD90、CD45表達(dá)，骨髓間充質(zhì)干細(xì)胞分別在分為低氧組(1%O2)和常氧組(20%O2)培養(yǎng)0-7天。根據(jù)細(xì)胞培養(yǎng)和沖涮時氧濃度，細(xì)胞粘附實驗分組為20%O2培養(yǎng)20%O2粘附?jīng)_涮組（A組）、1%O2培養(yǎng)1%O2粘附?jīng)_涮組（B組）、20%O2培養(yǎng)1%O2粘附?jīng)_涮組（C組）和1%O2培養(yǎng)20%O2粘附?jīng)_涮組（D組），比較不同氧濃度變化條件下細(xì)胞殘留率。研究不同氧濃度變化對間充質(zhì)干細(xì)胞表達(dá)HIF-1a，Integrin beta1，VCAM1和ICAM1的影響，實驗分為4組：1組:恒定20%O2培養(yǎng)；2組：恒定1%O2培養(yǎng)；3組：20%O2培養(yǎng)后于1%O2繼續(xù)培養(yǎng)2h；4組:1%O2培養(yǎng)后20%O2繼續(xù)培養(yǎng)2h。 結(jié)果流式細(xì)胞檢測99%以上實驗細(xì)胞表達(dá)CD54+、CD29+、CD90+，1%實驗細(xì)胞表達(dá)CD45+,證實細(xì)胞為骨髓間充質(zhì)干細(xì)胞。骨髓間充質(zhì)干細(xì)胞在1%O2環(huán)境下（A組和B組）粘附能力增強(qiáng)，，在20%O2環(huán)境下（C組和B組）粘附能力減弱（P0.05）；Integrin beta1，HIF-1a表達(dá)水平在1%O2環(huán)境下（2組和3組）增強(qiáng)（P0.05）。 結(jié)論本研究發(fā)現(xiàn)骨髓間充質(zhì)干細(xì)胞隨著粘附時間越長，細(xì)胞粘附能力也逐漸增強(qiáng)，經(jīng)過低氧處理細(xì)胞的粘附力提高時間快于常氧環(huán)境
[Abstract]:Background Mesenchymal stem cells are non-hematopoietic stem cells with multiple differentiation potential. They have been widely used in stem cell transplantation, tissue engineering and organ transplantation immunotherapy. Especially as an important seed cell in the construction of tissue engineering blood vessel, but its mechanism is not completely clear. Objective to investigate the effect of oxygen concentration on the adhesion of rat bone marrow mesenchymal stem cells (BMSCs). Methods Bone marrow mesenchymal stem cells (BMSCs) were obtained from 20 SD rats, aged 6-8 weeks, weighing 100g-120g, and bone marrow mesenchymal stem cells (BMSCs) were isolated by adherent method. CD54, CD29 CD90 and CD45 expression were detected by flow cytometry. Bone marrow mesenchymal stem cells were cultured for 0-7 days in hypoxia group and normoxic group respectively. The cell adhesion test was divided into two groups: 20O 2 culture and 20% O 2 adhesion scouring group A: O 2 group 1 O 2 culture, 2 O 2 adhesion flushing group B group (n = 1) and 1 O 2 culture group 1 O 2 adhesion rinse group C group (C group) and 1 O 2 culture 20 times O 2 adhesion rinse group D group, the cells were compared under different oxygen concentration change conditions. To study the effects of different oxygen concentrations on the expression of HIF-1aHIF-1 integrin beta1 VCAM1 and ICAM1 in mesenchymal stem cells. The experiment was divided into 4 groups: group 1: group 2: group 2: group 2: group 2: group 2: group 2: group 3: group 1: 2: 2 after 2 h culture, group 1: 4, group 1: 1, group 2: 2, group 2: 2, group 2: 1, group 2: 1, group 2: 1, group 1: 1, group 2: 2: 2. Results flow cytometry was used to detect the expression of CD45 in more than 99% experimental cells, which confirmed that the cells were bone marrow mesenchymal stem cells. The adhesiveness of bone marrow mesenchymal stem cells was enhanced in group A and B under 1O 2 environment. The adhesion ability of group C and group B was decreased under the condition of 20O 2. The expression of P0.05 integrin beta1a HIF-1a was enhanced in group 2 and group 3 (P 0.05). Conclusion in this study, the adhesion ability of bone marrow mesenchymal stem cells increased with the increase of adhesion time, and the adhesion of cells treated with hypoxia was faster than that of normoxic environment.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R329.2

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相關(guān)期刊論文 前4條

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