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  本文選題：毒蕈堿受體　切入點：迷走神經(jīng)活性　出處：《廣東醫(yī)學》2017年S2期 　論文類型：期刊論文

【摘要】：目的探討中樞毒蕈堿受體(M受體)對大鼠迷走神經(jīng)傳出放電活性的影響。方法取健康成年雄性SD大鼠,隨機分為6組(每組6只):加蘭他敏組、中樞M受體拮抗組、新斯的明腹腔組、新斯的明側(cè)腦室組、M1受體激動組及M2受體拮抗組。將迷走神經(jīng)放電信號引導至BL-420F生理儀持續(xù)記錄,其中采樣速率1k Hz/s,濾波范圍100~1 000 Hz。穩(wěn)定記錄神經(jīng)放電信號10 min(作為基礎(chǔ)值)后,按照上述分組注射相應(yīng)藥物,并持續(xù)記錄迷走神經(jīng)傳出放電活性60 min。整個放電觀察周期內(nèi),每隔10 min為時間點,記錄放電頻率和峰值,并進行統(tǒng)計學處理。結(jié)果傳出放電頻率于加蘭他敏注射后17 min開始明顯增加,持續(xù)至觀察期結(jié)束。期間最高值為(516±40)Hz,最低值為(249±26)Hz,與給藥前相比差異均有統(tǒng)計學意義(P0.05)。腹腔注射加蘭他敏前給予硫酸阿托品干預處理后,各時間點放電頻率和峰值與給藥前比較無明顯改變(P0.05),放電圖內(nèi)僅觀察到散在爆發(fā)電位。腹腔注射新斯的明前10 min內(nèi)觀察,迷走神經(jīng)傳出放電頻率和峰值記錄無明顯變化(P0.05)。側(cè)腦室注射新斯的明前10 min內(nèi)觀察,放電頻率和峰值無明顯變化(P0.05)。放電頻率于新斯的明注射后13min開始明顯增加,持續(xù)至觀察期結(jié)束,與給藥前相比差異均有統(tǒng)計學意義(P0.05)。側(cè)腦室注射MCN-A-343前10 min內(nèi)觀察,放電頻率和峰值記錄無明顯變化。放電頻率于MCN-A-343注射后12 min開始明顯增加,持續(xù)至觀察期結(jié)束,與給藥前相比差異均有統(tǒng)計學意義(P0.05)。側(cè)腦室注射美索曲明前10 min內(nèi)觀察,迷走神經(jīng)傳出放電頻率和峰值無明顯變化,注射美索曲明后8 min左右,放電峰值顯著增加,持續(xù)48 min后回落至注射藥物前水平,期間各時間點,與給藥前相比差異均有統(tǒng)計學意義(P0.05)。放電頻率于美索曲明注射后17min開始明顯增加,持續(xù)至觀察期結(jié)束。與給藥前相比差異均有統(tǒng)計學意義(P0.05)。結(jié)論通過抑制中樞膽堿酯酶而提高乙酰膽堿含量,可增強迷走神經(jīng)的傳出活性;此過程可能是由中樞M1受體介導的;而中樞M2受體則可能抑制迷走神經(jīng)傳出活性。
[Abstract]:Objective to investigate the effect of muscarinic muscarinic receptor (muscarinic receptor) on the efferent discharge activity of vagus nerve in rats. Methods healthy adult male SD rats were randomly divided into 6 groups (6 rats in each group: Galantamine group, central M receptor antagonistic group). In neostigmine peritoneal group, neostigmine group, M 1 receptor stimulation group and M 2 receptor antagonism group, the vagus nerve discharge signals were directed to the BL-420F physiological apparatus for continuous recording. The sampling rate was 1k Hz / s and the filtering range was 100 Hz / s. The nerve discharge signals were recorded stably for 10 mins (as the base value), then the corresponding drugs were injected according to the above groups, and the efferent discharge activity of vagus nerve was recorded continuously for 60 mins. The discharge frequency and peak value were recorded every 10 min. Results the efferent discharge frequency increased significantly at 17 min after galantamine injection. The maximum value was 516 鹵40 Hz and the lowest value was 249 鹵26hz.There were significant differences between before and after administration of atropine sulfate before intraperitoneal injection of galantamine. There was no significant change in the frequency and peak value of discharge at each time point compared with that before administration, but only the scattered burst potential was observed in the discharges. 10 min before intraperitoneal injection of neostigmine, the discharges were observed within 10 min after intraperitoneal injection of neostigmine. There was no significant change in the frequency and peak value of vagus nerve efferent discharge (P 0.05). The discharge frequency and peak value of neostigmine were observed 10 min before injection of neostigmine. The frequency of discharge began to increase 13 minutes after neostigmine injection. There were significant differences between the two groups until the end of the observation period (P 0.05). The discharge frequency and peak value were not significantly changed within 10 min before intracerebroventricular injection of MCN-A-343. The frequency of discharge began to increase significantly at 12 min after MCN-A-343 injection. At the end of the observation period, the differences were statistically significant compared with those before administration. The frequency and peak value of vagal nerve efferent discharge did not change significantly within 10 min before injection of misotramine, and the frequency and peak value of vagal nerve efferent discharge did not change significantly, about 8 min after injection of misotremine. The peak value of discharge increased significantly, and decreased to the pre-injection level after 48 min. There was a significant difference between the two groups at different time points (P 0.05). The discharge frequency began to increase at 17 minutes after misotromine injection. Conclusion the efferent activity of vagus nerve can be enhanced by inhibiting central cholinesterase and increasing the efferent activity of vagus nerve, which may be mediated by central M1 receptor. Central M 2 receptor may inhibit the efferent activity of vagus nerve.
【作者單位】： 石河子大學醫(yī)學院基礎(chǔ)醫(yī)學系;石河子大學醫(yī)學院臨床醫(yī)學院;石河子大學醫(yī)學院第一附屬醫(yī)院神經(jīng)內(nèi)科;石河子大學醫(yī)學院第一附屬醫(yī)院心內(nèi)科;新疆醫(yī)科大學附屬腫瘤醫(yī)院影像中心CT室;
【分類號】：R338

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