本文選題：抽動障礙　切入點：動物模型　出處：《中國兒童保健雜志》2016年12期 　論文類型：期刊論文

【摘要】：目的對亞氨基二丙腈(IDPN)和2,5-二甲氧-4-碘苯-2氨基丙烷(DOI)兩種抽動障礙(TD)模型的行為學特征進行觀察,并測定其血漿及紋狀體中的多巴胺(DA)與5-羥色胺(5-HT)等神經遞質水平,為更好地選擇TD模型提供理論依據并揭示TD的發(fā)生機制。方法通過SD大鼠腹腔注射IDPN和DOI分別建立IDPN和DOI抽動障礙大鼠模型,應用雙盲法觀察記錄兩模型(大鼠)的行為學變化,從運動行為、刻板行為和分類刻板行為三個方面進行評估和比較。采用酶聯免疫吸附試驗(ELISA)檢測兩種模型鼠大腦紋狀體和血漿中DA與5-HT等神經遞質的含量,探討IDPN與DOI動物模型的行為學特征及機制。結果 IDPN組大鼠和DOI組大鼠的運動行為評分和刻板行為評分均高于對照組,差異具有統(tǒng)計學意義(P0.05);IDPN組大鼠的旋轉、舞蹈樣運動高于對照組和DOI組,DOI組大鼠的口爪運動、自咬高于對照組和IDPN組,差異均具有統(tǒng)計學意義(P0.05)。IDPN組大鼠血漿及紋狀體中的DA(5.70±3.12,137.45±20.14)明顯高于對照組(0.32±0.12,68.13±12.34)和DOI組(1.01±0.74,88.56±21.30),差異具有統(tǒng)計學意義(F=13.43~8.6,P0.05)。DOI組大鼠紋狀體中5-HT(56.83±34.72)明顯低于對照組(109.14±14.05)和IDPN組(72.52±10.03),差異具有統(tǒng)計學意義(F=3.65,P0.05)。結論 IDPN模型主要表現為全身性抽動,DOI模型主要以局部抽動為主。IDPN模型可能通過影響DA系統(tǒng),而DOI模型則可能通過激活5-HT受體系統(tǒng),從而引起大鼠出現抽動癥狀。
[Abstract]:Objective to observe the behavioral characteristics of IDPN and 2-dimethoxy-4-iodobenzene-2-aminopropane (DOI) models, and to determine the levels of dopamine (DA) and serotonin (5-HT) in plasma and striatum. Methods SD rats were injected intraperitoneally with IDPN and DOI to establish IDPN and DOI dystic rat models, respectively, in order to provide theoretical basis for better selection of TD model and reveal the mechanism of TD. Two blind methods were used to observe and record the behavioral changes of the two models (rats). The levels of DA and 5-HT neurotransmitters in striatum and plasma of two kinds of model rats were measured by enzyme linked immunosorbent assay (Elisa). Results the scores of motor behavior and stereotypical behavior in IDPN group and DOI group were higher than those in control group, and the difference was statistically significant. Dance-like exercise was higher than that in control group and DOI group, and self-biting was higher than that in control group and IDPN group. The difference was statistically significant in plasma and striatum (DA(5.70 鹵3.1277.45 鹵20.14) of rats in P0.05 + IDPN group, 0.32 鹵0.128.13 鹵12.34 in control group and 1.01 鹵0.74 鹵88.56 鹵21.30 in DOI group. The difference was statistically significant in F13.438.6P0.05P0.05.DOI group (56.83 鹵34.72) compared with control group (109.14 鹵14.05) and IDPN group (72.52 鹵10.03g), and the difference was statistically significant compared with that in control group (109.14 鹵14.05) and IDPN group (72.52 鹵10.03). Conclusion the main manifestation of IDPN model is that the local twitch is the main model. IDPN model may influence DA system. The DOI model may activate 5-HT receptor system, which may induce tic symptoms in rats.
【作者單位】： 青島大學附屬醫(yī)院兒童保健科;煙臺毓璜頂醫(yī)院兒科;福建省立醫(yī)院兒科;青島大學附屬醫(yī)院遺傳室;
【基金】：國家自然科學基金資助項目(81101017;81371499)
【分類號】：R749.94;R-332

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