本文選題：家蠅　切入點：腸道共生細(xì)菌　出處：《中國病原生物學(xué)雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的了解進(jìn)食對家蠅成蟲腸道共生菌數(shù)量和種類的影響。方法剛羽化的家蠅隨機(jī)分為正常喂食組和未喂食組,每天分雌、雄取樣,解剖腸道后,分離其腸道內(nèi)細(xì)菌,直到全部死亡。家蠅腸道共生細(xì)菌采用傳統(tǒng)方法分離培養(yǎng)。挑取形態(tài)有差異的單菌落,于LB培養(yǎng)基中搖菌過夜,提取DNA,進(jìn)行16srDNA基因擴(kuò)增,擴(kuò)增產(chǎn)物測序,并在NCBI中進(jìn)行序列比對,鑒定到屬,計數(shù)家蠅不同蟲態(tài)腸道中分離到的細(xì)菌菌屬數(shù),分析其變化。結(jié)果在家蠅成蠅腸道內(nèi)共分離到共生菌17屬,其中正常進(jìn)食成蠅分離到12屬,未進(jìn)食成蠅腸離到10屬,兩組共有細(xì)菌6屬,分別為普羅威登斯菌屬、葡萄球菌屬、香味菌屬、克雷伯菌屬、假單胞菌屬和不動桿菌屬。正常喂食的家蠅腸道中特有的細(xì)菌有7屬,分別為蒼白桿菌屬、鞘氨醇桿菌屬、腸球菌屬、寡養(yǎng)單胞菌屬、土壤桿菌、代爾夫特菌屬和漫游球菌屬;未喂食家蠅腸道中特有的細(xì)菌有4屬,分別為白色桿菌屬、腸桿菌屬、短狀桿菌屬和微桿菌屬。正常喂食組不同日齡雌蟲和雄蟲腸道中分離到的共生細(xì)菌菌屬數(shù)量差異有統(tǒng)計學(xué)意義(F值分別為5.57和3.57,P0.05或P0.01);未進(jìn)食組不同日齡雌蟲和雄蟲腸道中分離到的共生細(xì)菌菌屬數(shù)量差異無統(tǒng)計學(xué)意義(F值分別為0.17和0.92,P0.05)。結(jié)論進(jìn)食不但可影響家蠅的壽命,還可影響家蠅成蟲體內(nèi)的腸道共生細(xì)菌的組成,推測其腸道共生細(xì)菌可能部分來自于食物和生活環(huán)境。
[Abstract]:Objective to investigate the effect of feeding on the number and species of intestinal symbiotic bacteria in adults of housefly. Methods the newly emerging houseflies were randomly divided into normal feeding group and non-feeding group. The intestinal symbiotic bacteria of Musca domestica were isolated and cultured by traditional methods. The single colony with different morphology was picked up. The bacteria were swayed overnight in LB medium, the DNA was extracted, the 16s rDNA gene was amplified, and the amplified products were sequenced. The results showed that 17 genera of symbiotic bacteria were isolated in the intestinal tract of housefly, and 12 genera were isolated from normal feeding adult fly, and the number of bacterial genera isolated from the intestinal tract of housefly was counted and analyzed by sequence alignment in NCBI, and the results showed that there were 17 genera of symbiotic bacteria in the intestinal tract of housefly adult flies, among which 12 genera were isolated from normal feeding adult flies. There were 6 genera of bacteria in the two groups, I. e., Providence, Staphylococcus, Flavor, Klebsiella. Pseudomonas and Acinetobacter. There are 7 genera of bacteria endemic to the intestinal tract of housefly, I. E. pallidus, sphingosine, Enterococcus, Oligomonas, Agrobacterium, Delft's and rover. There are 4 genera of bacteria that are endemic to the intestinal tract of housefly, namely, Bacillus albicans and Enterobacter. The numbers of symbiotic bacteria isolated from the intestines of females and males in normal feeding group were 5.57 and 3.57 respectively, and those of male and female were 5.57 and 3.57 respectively. There was no significant difference in the number of symbiotic bacteria isolated from the intestinal tract of insects. The F values were 0.17 and 0.92 P0.05respectively. Conclusion eating food can not only affect the lifespan of Musca domestica, but also affect the life span of Musca domestica. It can also affect the composition of intestinal symbiotic bacteria in adults of Musca domestica, suggesting that the symbiotic bacteria may partly come from food and living environment.
【作者單位】： 泰山醫(yī)學(xué)院新發(fā)傳染病溯源及防控協(xié)同創(chuàng)新中心;泰山醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金項目(No.81572028,81401693,81271874) 山東省科技發(fā)展計劃項目(No.2014GSF121007)
【分類號】：R384.2

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